Endocrinology, Vol 127, 3234-3236, Copyright © 1990 by Endocrine Society

ARTICLES

Localization of insulin receptor mRNA in rat brain by in situ hybridization

JL Marks, D Porte Jr, WL Stahl and DG Baskin
Division of Endocrinology/Metabolism, Medical Research Service, Veterans Affairs Medical Center, Seattle, Washington 98108.

Insulin receptor mRNA was demonstrated in rat brain slices by in situ hybridization with three 35S-oligonucleotide probes and contact film autoradiography. Specificity was confirmed by showing that (a) excess unlabeled probe abolished the signal, (b) an oligonucleotide probe for rat neuropeptide Y mRNA showed a different distribution of hybridization signal, and (c) the distribution of insulin receptor binding was consistent with the distribution of insulin receptor mRNA. Insulin receptor mRNA was most abundant in the granule cell layers of the olfactory bulb, cerebellum and dentate gyrus, in the pyramidal cell body layers of the pyriform cortex and hippocampus, in the choroid plexus and in the arcuate nucleus of the hypothalamus.

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