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Analysis of the Steroid Binding Domain of Rat Androgen-Binding Protein^*

Departments of Obstetrics and Gynecology, Biochemistry, and The Center for Reproductive Biology Research Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2633
Departments of Pharmacology Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2633
Departments of Pharmacology, and The Center for Reproductive Biology Research Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2633

Address correspondence and requests for reprints to: Benjamin J. Danzo, Ph.D., Department of Obstetrics and Gynecology, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232-2633.

Abstract

The site-directed photoaffinity ligand [3H]17β-hydroxy-4,6-androstadien-3-one (⁶-testosterone) was used to label the steroid binding domain of rat androgen-binding protein (rABP). After digestion with trypsin, the major radiolabeled peptide was isolated by reverse phase chromatography. The peptide was found to have the following amino acid sequence: He Ala Leu Gly Gly Leu Leu Leu Pro Thr Ser. Gaps in the sequence that one would anticipate if ⁶-testosterone formed an adduct with a single amino acid were not encountered. Several different amino acids appear to have been labeled as expected given the free radical nature of photoactivated ⁶-testosterone.The sequence obtained corresponded to a tryptic peptide (amino acids 171–181) of the rABP precursor. The only other protein having this amino acid sequence was human sex hormone binding globulin. The binding domain lies in a hydrophobic pocket that contains a predicted β-sheet and turn secondary structure, as would be anticipated given the hydrophobic nature of the steroid molecule. A hydropathy and secondary structure analysis of rABP was performed as a basis for discussing the results of the current study in relation to previous studies on the steroid binding domain on human sex hormone binding globulin.

Footnotes

^* This work was supported by NIH Grants HD-13813 (to B.J.D.) and HD-20583 (to M.K.S.) and by a grant from the Andrew W. Mellon Foundation (to B.J.D.). The Center for Reproductive Biology Research is supported by NIH Grant HD-05797.

Received February 14, 1991.

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