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Effect of Prostaglandin F₂ on Cytosolic Free Calcium Ion Concentrations in Rat Luteal Cells^*

Departments of Obstetrics and Gynecology, University of British Columbia Vancouver, British Columbia, Canada
Physiology, University of British Columbia Vancouver, British Columbia, Canada

Address all correspondence and requests for reprints to: Dr. Peter C. K. Leung, Department of Obstetrics and Gynecology, University of British Columbia, Room 2H30, Grace Hospital, 4490 Oak Street, Vancouver, British Columbia, Canada V6H 3V5.

Abstract

Changes in cytosolic free calcium concentrations ([Ca²⁺]i) in response to prostaglandin F₂ (PGF₂) were measured in single rat luteal cells, using the calcium-sensitive fluorescent dye fura-2. A total of 112 cells were studied in 20 experiments. The average resting [Ca²⁺]i was 113 ± 6.4 nM. In 59 cells (53%), there was a 4.6 ± 0.2-fold increase in [Ca²⁺]i within 29.3 ± 1.0 sec of PGF₂ administration, and the cells recovered by 78.0 ± 4.5 sec. The magnitude of the increase in [Ca²⁺]i in response to PGF₂ was not changed by an increase in the concentration of PGF₂. Perifusion with low calcium buffer reduced, then eliminated, the [Ca²⁺]i response to PGF₂. Perifusion of cells with PGF₂ resulted in a single transient [Ca²⁺]i response, similar to that after short term exposure to PGF₂. Many (67%) of the cells that responded to PGF₂ also responded to GnRH. No additive increase in [Ca²⁺]i was seen when PGF₂ and GnRH were administered together. The source of the calcium appears to be intracellular stores that are shared by GnRH and PGF₂.

Footnotes

^* This work was supported by grants from the Medical Research Council of Canada (to K.G.B. and P.C.K.L)

Received February 2, 1991.

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