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Expression and Regulation of Growth Hormone (GH) Receptor Messenger Ribonucleic Acid (mRNA) in Rat Adipose Tissue, Adipocytes, and Adipocyte Precursor Cells: GH Regulation of GH Receptor mRNA^*

Department of Physiology, University of Göteborg S-400 33 Göteborg, Sweden

Address all correspondence and requests for reprints to: Dr. Staffan Edén, Department of Physiology, University of Göteborg, P.O. Box 33031, S-400 33 Göteborg, Sweden.

Abstract

The effects of hypophysectomy and hormonal replacement therapy on GH receptor (GH-R) gene expression was studied in rat adipose tissue with a cRNA probe corresponding to the amino-terminal of the hepatic GH-R. Male Sprague-Dawley rats, 50–65 days of age, were used. In all fat depots tested (epididymal, retroperitoneal, and sc), two transcripts with an estimated size of 4.0 and 1.2 kilobases (kb), respectively, were detected. An intermediate-size transcript (2.6 kb) was sometimes observed. Also, isolated adipocytes and adipocyte precursor cells from the epididymal fat pad expressed these GH-R transcripts. The pituitary dependence of GH-R gene expression was analyzed in epididymal fat. Hypophysectomies were performed at 50 days of age, and the rats were then given replacement therapy with L-T₄ (10 µg/kg·day) and hydrocortisone (400 µg/kg·day). Hypophysectomy decreased the abundance of both the 4.0 and the 1.2-kb transcripts, an effect that in part was restored by GH treatment.

A solution hybridization RNase protection assay was then used to further characterize the effect of GH treatment of hypophysectomized rats on GH-R gene expression. A single injection of human GH (100 µg/rat) increased GH-R mRNA levels within 1 h, and maximal levels were reached between 3–12 h after the injection. The increase in GH-R mRNA levels was dose dependent and was observed also after prolonged treatment (1 or 5 mg/kg·day for 6 days) with bovine GH.

These results confirm that GH-R mRNAs are present in rat adipose tissue from different fat depots. GH-R transcripts of the same estimated size were detected in isolated adipocytes and adipocyte precursor cells. Furthermore, the results show that there is a rapid and GH-dependent regulation of GH-R mRNA levels in adipose tissue. (Endocrinology 129: 1155–1161, 1991)

Footnotes

^* This research was supported by the Swedish Medical Research Council (Grant 8269), Nordisk Insulinfond, the Göteborg Medical Society, the Faculty of Medicine, University of Göteborg, and the Swedish Society of Medical Research.

Received December 26, 1990.

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