help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nakatani, A.
Right arrow Articles by Ling, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakatani, A.
Right arrow Articles by Ling, N.

Endocrinology, Vol 129, 1521-1529, Copyright © 1991 by Endocrine Society

ARTICLES

Tissue-specific expression of four insulin-like growth factor-binding proteins (1, 2, 3, and 4) in the rat ovary [published erratum appears in Endocrinology 1992 Nov;131(5):2350]

A Nakatani, S Shimasaki, GF Erickson and N Ling
Department of Molecular Endocrinology, Whittier Institute for Diabetes and Endocrinology, La Jolla, California 92093.

We investigated the possible presence of the mRNAs encoding four insulin-like growth factor-binding proteins (IGFBP-1, -2, -3, and -4) in the rat ovary. It has been demonstrated previously by Northern analysis that adult rat ovaries contain mRNA transcripts for IGFBP-2 and -3. Here we show by Northern analysis that adult rat ovaries contain mRNA for IGFBP-4, but the mRNA for IGFBP-1 was below the limit of detection. Using in situ hybridization, IGFBP-1 mRNA was not detected in any of the ovaries tested. The IGFBP-2 mRNA was localized specifically in thecal interstitial cells (TIC) and secondary interstitial cells of all ovaries. The IGFBP-2 signal was very strong in TIC of all Graafian follicles (healthy and atretic) and very strong in all secondary interstitial cells located in different regions of the ovary, but weak in TIC of preantral follicles. The IGFBP-3 hybridization signal was localized specifically to some corpora lutea. Here the hybridization single for IGFBP-3 was strong and distributed throughout the corpus luteum, strong but localized to only some luteal cells, or not detectable. The IGFBP-4 mRNA was localized almost exclusively to granulosa cells of atretic follicles. The intensity of the IGFBP-4 signals appeared to increase as the level of atresia increased. In some cases, IGFBP-4 signals were detected in the TIC, but they were weak and variable. These results show that the mRNAs for IGFBP-2, -3, and -4 are localized to the interstitial cells, corpora lutea, and atretic granulosa cells, respectively. The tissue-specific synthesis of IGFBP subtypes in specialized ovarian cells provides an excellent system to study the manner in which IGFBP synthesis is controlled and their potential role as an autocrine and paracrine factors.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1991 by The Endocrine Society