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*
Pediatric Endocrinology Unit, Karolinska Hospital S-10401 Stockholm, Sweden
the Department of Anatomy, University of Turku SF-20520 Turku, Finland
Address all correspondence and requests for reprints to: Martti Parvinen, Department of Anatomy, University of Turku, Kiinamyllynkatu 10, SF-20520 Turku, Finland.
Abstract
Levels of rat testicular interleukin-1-like factor (tIL-1) have been shown to correlate with DNA synthetic activity during the cycle of the rat seminiferous epithelium, suggesting its role as a spermatogonial or meiotic growth factor. To explore this further, a new in vitro model system was developed. Rat seminiferous tubule segments from stages I, V, Vila, and VIII IX of the cycle were isolated by transillumination-assisted microdissection, cultured in chemically defined serum-free medium supplemented with human recombinant IL-1
, and labeled with [3H]thymidine.
During incubation, spontaneous progression of spermatogenesis was noted. Inactive stage Vila tubule segments differentiateddifferentiated to stage VIII and initiated DNA synthesis, and concomitantly started to secrete IL-1-like factor. DNA synthesis of stages VIII-IX ceased through differentiation of spermatocytes to leptotene- zygotene (stages XII-XIII of the cycle). IL-1 stimulated DNA synthesis significantly in spermatogonia of stage I. Meiotic DNA synthesis at stage Vila was stimulated (48 h/34 C) and maintained at stages VIII-IX (48 h/34 C).
IL-1 seems to act as a regulator of spermatogenic DNA synthesis in both mitotic and meiotic phases. It has mainly stimulating and maintaining effects, but it may also be inhibitory under certain conditions. (Endocrinology 129: 1614–1620,1991)
Footnotes
* This work was supported by grants from the Academy of Finland, Karolinska Institute, Swedish Medical Research Council, the King Gustav V Jubilee Foundation, and the Childrens Cancer Foundation.
Received February 21, 1991.
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