This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by LOTSHAW, D. P. Articles by MULROW, P. J. Search for Related Content PubMed PubMed Citation Articles by LOTSHAW, D. P. Articles by MULROW, P. J.

Atrial Natriuretic Peptide Inhibition of Calcium Ionophore A23187-Stimulated Aldosterone Secretion in Rat Adrenal Glomerulosa Cells^*

DAVID P. LOTSHAW, ROBERTO FRANCO-SAENZ and PATRICK J. MULROW

Department of Medicine, Medical College of Ohio Toledo, Ohio 43699

Address all correspondence and requests for reprints to: Dr. Patrick J. Mulrow, Department of Medicine, Medical College of Ohio, CS 10008, Toledo, Ohio 43699.

Abstract

The effect of atrial natriuretic peptide (ANP) on calcium ionophore A23187-stimulated aldosterone secretion was investigated using collagenase-dispersed rat adrenal glomerulosa cell suspensions. A23187 treatment induced a dose-dependent stimulation of aldosterone secretion, exhibiting an EC₅₀ of approximately 75 nM. In agreement with the presumed action of A23187 as a Ca²⁺ ionophore, stimulation was dependent on the extracellular Ca²⁺ concentration, being completely inhibited in nominally Ca²⁺-free medium. In such Ca²⁺-free medium, stimulation of aldosterone secretion by bath applied 25-hydroxycholesterol was not inhibited, indicating that cells and biosynthetic pathway enzymes were not inhibited by low extracellular Ca²⁺ levels. A23187-induced aldosterone secretion was also inhibited by more than 90% when cells were simultaneously treated with ANP. Maximal ANP inhibition of A23187-stimulated aldosterone secretion was not overcome by concentrations of A23187 up to 10 µM or by increasing the extracellular Ca²⁺ concentration from 1.25 to 5 mM in the presence of A23187 and ANP. Addition of A23187 to ACTH-, angiotensin II-, or K⁺-stimulated glomerulosa cells did not overcome ANP-induced inhibition of aldosterone secretion stimulated by these secretagogues. In contrast to ANP inhibition of Ca²⁺-dependent A23187 stimulation of aldosterone secretion, ANP inhibition of dBcAMP-stimulated aldosterone secretion was readily overcome by increasing the dBcAMP concentration. These results indicated that ANP selectively and noncompetetively inhibited an intracellular step necessary for Ca²⁺-dependent stimulation of the early pathway of aldosterone biosynthesis in rat adrenal glomerulosa cells. (Endocrinology 129: 2305–2310, 1991)

Footnotes

^* An abstract of these data was published previously in the Program of the 72nd Annual Meeting of The Endocrine Society, Atlanta, GA, 1990, p. 399. This work was supported in part by a grant from the American Heart Association, Ohio Affiliate, Inc. (Columbus, OH).

T. H. Morgan School of Biological Sciences, University of Kentucky, Lexington, Kentucky 40506–0225.

Received May 6, 1991.

This article has been cited by other articles:

				M. D. Payet, T. L. Goodfriend, L. Bilodeau, C. Mackendale, L. Chouinard, and N. Gallo-Payet An oxidized metabolite of linoleic acid increases intracellular calcium in rat adrenal glomerulosa cells Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1160 - E1167. [Abstract] [Full Text] [PDF]

				S. Takida, B. J. Elmquist, and G. J. Trachte C-Type Natriuretic Peptide Attenuates Evoked Dopamine Efflux by Influencing Go{alpha} Hypertension, January 1, 1999; 33(1): 124 - 129. [Abstract] [Full Text] [PDF]

				S. Kanwal, B. J. Elmquist, and G. J. Trachte Atrial Natriuretic Peptide Inhibits Evoked Catecholamine Release by Altering Sensitivity to Calcium J. Pharmacol. Exp. Ther., November 1, 1997; 283(2): 426 - 433. [Abstract] [Full Text]

				D. P. Lotshaw Effects of K+ Channel Blockers on K+ Channels, Membrane Potential, and Aldosterone Secretion in Rat Adrenal Zona Glomerulosa Cells Endocrinology, October 1, 1997; 138(10): 4167 - 4175. [Abstract] [Full Text] [PDF]