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Estradiol Negatively Regulates Secretogranin II and Chromogranin A Messenger Ribonucleic Acid Levels in the Female Rat Pituitary but Not in the Adrenal

Laboratoire d’Endocrinohgie Moleculaire (Y.A., T.B., M.M., J.D.), Centre National de la Recherche Scientifique, Unite de Recherche Associee 256, Campus de Beaulieu 35042 Rennes, France
Laboratoire des Hormones Polypeptidiques, Centre National de la Recherche Scientifique (R.C) 91198 Gif-sur-Yuette, France

Address all correspondence and requests for reprints to: Youssef Anouar, Universite de Rennes I, Laboratoire d’Endocrinologie Moleculaire, Campus de Beaulieu, 35042 Rennes Cedex, France.

Abstract

Estradiol (E₂) effects on the pituitary and adrenal secretogranin II (Sgll) and chromogranin A (CgA) proteins and mRNA levels were analyzed in the adult female rat. Animals were ovariectomized or sham-operated for 2 weeks and then daily injected with various doses of 17β-E₂ (from 5–100 µg) for the following week. Sgll and CgA levels were determined by Western blot analysis using two specific antisera. Messenger RNA (mRNA) levels were measured by RNA slot blot analysis using specific cDNA probes. Simultaneously, pituitary LH content and gonadotropin subunit (LHβ, FSHβ, ) mRNAs were quantified. Ovariectomy promoted a significant increase in pituitary Sgll and CgA proteins (2-fold Vs. sham-operated animals, P < 0.01) and a concomitant rise in their mRNA levels (2.5-fold and 4.5-fold for Sgll mRNA and CgA mRNA, respectively, P < 0.01). In the same animals LHβ, FSHβ, and -subunit mRNA levels increased by 20-, 12-, and 6-fold, respectively. Estrogen replacement resulted in a parallel decrease of CgA and LHβ mRNA to the control values, starting from the lowest steroid dose (5 µg). The Sgll mRNA decrease was initiated only with a higher concentration of E₂ (10 µg), as was that of -subunit mRNA; yet, the Sgll mRNA level remained significantly higher than in the control pituitary, even with the highest steroid dose (P < 0.05) at variance with the -mRNA level. Concerning protein concentration, the postcastration increase in CgA was fully reverted with 10 µg E₂ while that of Sgll remained unaffected, as was the pituitary LH content. In the adrenal gland, neither the ovariectomy nor the E₂ therapy altered significantly the Sgll or CgA protein and mRNA concentrations. We conclude that, in rats, 1) ovarian factors regulate the pituitary Sgll and CgA protein and mRNA steady-state levels while such factors are inefficient in the adrenal gland, 2) CgA and LHβ mRNAs exhibit the same sensitivity to E₂ while Sgll and -subunit mRNAs appear less sensitive, and 3) Sgll and LH pituitary contents present a similar pattern of variations when the estrogenic status of the animal is modified. (Endocrinology 129: 2393–2399,1991)

Received April 25, 1991.

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