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Messenger Ribonucleic Acid Expression in Primary Cultures of Leydig Cells*Medical and Research Services, W. J. B. Dorn Veterans Hospital, and the Departments of Medicine, Microbiology, and Immunology, University of South Carolina School of Medicine Columbia, South Carolina 29208
Address requests for reprints to: Tu Lin, M.D., Medical Service, W. J. B. Dorn Veterans Hospital, Columbia, South Carolina 29201.
Abstract
Previously, we have reported that interleukin-1 (IL-1) can modulate Leydig cell steroidogenesis. Recently, IL-1-like material has been shown to be present in the testis; however, the cellular source of this material remains unclear. In the present study we found that human recombinant IL-1β (1–100 ml/ng) caused dose-dependent increases in IL-1
mRNA expression in Leydig cells. Similar to that reported in other tissues, IL-1 mRNA from Leydig cells is mainly 2.2 kilobases. IL-1 mRNA expression in Leydig cells was detectable as early as 2 h after the addition of IL-1β (10 ng/ml) and persisted for up to 24 h. Lipopolysaccharide also stimulated IL-1 mRNA expression in these cells, but phorbol ester had no effect. Our results indicate that Leydig cells are a potential source of IL-1, which has both autocrine and paracrine effects. (Endocrinology 129: 2862–2866, 1991)
Footnotes
* This work was supported by NIH Grant 1RO1-HD-25641 and the Department of Veterans Affairs Medical Research Fund (to T.L.).
Received July 9, 1991.
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