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Prolonged Fasting Reduces Rat Hepatic β1 Thyroid Hormone Receptor Protein without Changing the Level of Its Messenger Ribonucleic Acid^*

JAMES T. LANE, MADAN GODBOLE, KEVIN A. STRAIT, HAROLD L. SCHWARTZ and JACK H. OPPENHEIMER

Section of Endocrinology and Metabolism, Department of Medicine, University of Minnesota Minneapolis, Minnesota 55455

Address all correspondence and requests for reprints to: Dr. Jack H. Oppenheimer, Division of Endocrinology and Metabolism, Department of Medicine, University of Minnesota, Box 91 Mayo, 515 Delaware Street SE, Minneapolis, Minnesota 55455.

Abstract

The level of hepatic nuclear T₃-binding capacity falls in rats subjected to fasting. To define the mechanism underlying these changes, we have assayed in liver the concentration of the mRNA coding for the β1-receptor (β1-TR) isoform, the total nuclear T₃-binding capacity, and the fraction of the total binding capacity that can be specifically immunoprecipitated with an anti-β1-TR immunoglobulin G preparation. Although no changes in β1-TR mRNA concentration were noted, we observed a 60% fall in total binding capacity. β1-TR mRNA levels were preserved despite a 50% fall in total poly(A)⁺ RNA. The fall in β1-TR protein, however, was consistent with a generalized decrease in total hepatic protein content. This study provides yet another instance in which measurement of receptor mRNA is not consonant with the behavior of the nuclear T₃ receptor protein. (Endocrinology 129: 2881β2885, 1991)

Footnotes

^* This work was supported by NIH Grant DK-19812 (to J.H.O.), National Research Scientist Award DK-08066 (to K.A.S.), and National Research Scientist Award DK-08427 (to J.T.L.).

Supported by a Boyscast Fellowship (SP/BY/024/87) from the Department of Science and Technology, Government of India.

Received June 20, 1991.

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