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L-Arginine Stimulates Cyclic Guanosine 3',5'- Monophosphate Formation in Rat Islets of Langerhans and RINm5F Insulinoma Cells: Evidence for LArgininerNitric Oxide Synthase^*

Department of Pharmacology and Therapeutics, State University of New York School of Medicine and Biomedical Sciences Buffalo, New York 14214

Address all correspondence and requests for reprints to: Dr. Laychock, 102 Farber Hall, Department of Pharmacology and Therapeutics, State University of New York School of Medicine, Buffalo, New York 14214.

Abstract

L-Arginine (L-Arg) is metabolized by nitric oxide synthase to the reactive intermediate nitric oxide. Since nitric oxide stimulates guanylyl cyclase and cGMP synthesis, LArg effects on cGMP accumulation in isolated pancreatic islets of the rat and RINm5F insulinoma cells were determined. Both L-Arg and glucose stimulation increased islet cGMP levels, and glucose potentiated the response to L-Arg alone. A competitive inhibitor of L-Arg metabolism to nitric oxide,N^G-monomethyl- L-arginine, reduced glucose- and L-Arg-stimulated insulin release and glucose-induced increases in cGMP; however, basal insulin release was slightly increased. D-Arg and L-ornithine did not affect islet cGMP levels, although insulin release was stimulated. RINm5F cell cGMP levels and insulin release increased in response to L-Arg in a concentration- and time-related manner, whereas glucose and L-histidine were without effect. 8- Bromo-cGMP also slightly increased RINm5F cell insulin release. Sodium nitroprusside as a source of nitric oxide increased RINm5F cell cGMP production. Methylene blue and LY83583, inhibitors of soluble guanylyl cyclase activation, reduced RINm5F cell cGMP levels in the presence and absence of L-Arg; LY83583 also reduced glucose-stimulated cGMP levels in islets. Insulin release by glucose and L-Arg was also inhibited by methylene blue and LY83583 in islets. We conclude that glucose and L-Arg stimulate guanylyl cyclase activity and cGMP formation in β-cells at least in part through metabolism to the reactive intermediate nitric oxide. However, neither nitric oxide nor cGMP synthesis is obligatory for insulin secretion. (Endocrinology 129: 3043–3052, 1991)

Footnotes

^* This work was supported by NIH Grant AM-25705.

Received May 13, 1991.

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