help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology, doi:10.1210/endo-129-6-3313
Endocrinology Vol. 129, No. 6 3313-3320
Copyright © 1991 by the Endocrine Society.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by OURSLER, M. J.
Right arrow Articles by SPELSBERG, T. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by OURSLER, M. J.
Right arrow Articles by SPELSBERG, T. C.

Modulation of Transforming Growth Factor-β Production in Normal Human Osteoblast-Like Cells by 17β-Estradiol and Parathyroid Hormone

MERRY JO OURSLER, CASE CORTESE, PHILIP KEETING, MARLYS A. ANDERSON, SUSAN K. BONDE, B. LAWRENCE RIGGS and THOMAS C. SPELSBERG

Department of Biochemistry and Molecular Biology and the Endocrine Research Unit, Mayo Clinic and Mayo Foundation Rochester, Minnesota 55905

Address all correspondence and requests for reprints to: Dr. Thomas 0. Spelsberg, Mayo Clinic, 200 First Street SW, Rochester, Minnesota 55905.

Abstract

Although our laboratory has reported that normal human osteoblast-like (hOB) cells contain estrogen receptors, we have failed to find major effects of 17β-estradiol (E2) on modulation of proliferation of bone matrix protein production by hOB cells. Because the major effect of E2 in vivo is to decrease bone resorption and because transforming growth factor-β (TGF-β) has been reported to decrease osteoclast-mediated bone resorption, we have tested the hypothesis that the effect of E2 on osteoclast activity is, at least in part, indirectly mediated by enhancing production of TGF-β by osteoblasts. We therefore have extended our studies to examine possible TGF-β gene expression including the modulation of the release of TGF-β by E2 in near homogenous populations of hOB cells. TGF-β protein production was measured using growth inhibition of CCL-64 cells and verified by blocking effects with anti-TGF-β antibodies. TGF-β messenger RNA (mRNA) steady state levels were assessed by northern blot analysis and quantitated by densitometric measurement using 18S ribosomal RNA as a reference. There was an E2 dose-dependent increase in TGF-β protein production within 24 h of challenge with E2. Northern blots from these cells demonstrated a dose-dependent increase steady state mRNA levels of TGF-β within 6 h of treatment. PTH was also a potent stimulator of TGF-β protein and message levels in a dose-dependent manner. Interestingly, coincubation of equimolar concentrations of E2 and PTH (10–8 M) abrogated the stimulation of TGF-β mRNA and protein. Decreasing the relative concentration of PTH in this coincubation with increased TGF-β mRNA and protein levels. These data support the fact that E2 modulates TGF-β production in osteoblasts. this manner TGF-β may mediate E2 inhibition of osteoclast activity. (Endocrinology 129: 3313–3320, 1991)

Received June 22, 1991.




This article has been cited by other articles:


Home page
 J R Soc InterfaceHome page
L. M. McNamara
Perspective on post-menopausal osteoporosis: establishing an interdisciplinary understanding of the sequence of events from the molecular level to whole bone fractures
J R Soc Interface, October 21, 2009; (2009) rsif.2009.0282v1.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
R. H. Straub
The Complex Role of Estrogens in Inflammation
Endocr. Rev., August 1, 2007; 28(5): 521 - 574.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
U.H. Lerner
Bone Remodeling in Post-menopausal Osteoporosis
Journal of Dental Research, July 1, 2006; 85(7): 584 - 595.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
K. Janssens, P. ten Dijke, S. Janssens, and W. Van Hul
Transforming Growth Factor-{beta}1 to the Bone
Endocr. Rev., October 1, 2005; 26(6): 743 - 774.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Gharaee-Kermani, K. Hatano, Y. Nozaki, and S. H. Phan
Gender-Based Differences in Bleomycin-Induced Pulmonary Fibrosis
Am. J. Pathol., June 1, 2005; 166(6): 1593 - 1606.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. E. Koblinski, J. Dosescu, M. Sameni, K. Moin, K. Clark, and B. F. Sloane
Interaction of Human Breast Fibroblasts with Collagen I Increases Secretion of Procathepsin B
J. Biol. Chem., August 23, 2002; 277(35): 32220 - 32227.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Yamamoto, F. Saatcioglu, and T. Matsuda
Cross-Talk between Bone Morphogenic Proteins and Estrogen Receptor Signaling
Endocrinology, July 1, 2002; 143(7): 2635 - 2642.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
B. L. Riggs, S. Khosla, and L. J. Melton III
Sex Steroids and the Construction and Conservation of the Adult Skeleton
Endocr. Rev., June 1, 2002; 23(3): 279 - 302.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. Pfeilschifter, R. Koditz, M. Pfohl, and H. Schatz
Changes in Proinflammatory Cytokine Activity after Menopause
Endocr. Rev., February 1, 2002; 23(1): 90 - 119.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B.-Y. Liu, P.-W. Wu, F. R. Bringhurst, and J.-T. Wang
Estrogen Inhibition of PTH-Stimulated Osteoclast Formation and Attachment in Vitro: Involvement of Both PKA and PKC
Endocrinology, February 1, 2002; 143(2): 627 - 635.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Saika, D. Inoue, S. Kido, and T. Matsumoto
17{beta}-Estradiol Stimulates Expression of Osteoprotegerin by a Mouse Stromal Cell Line, ST-2, via Estrogen Receptor-{{alpha}}
Endocrinology, June 1, 2001; 142(6): 2205 - 2212.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. E. Compston
Sex Steroids and Bone
Physiol Rev, January 1, 2001; 81(1): 419 - 447.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
K. L. Blackwell, Z. A. Haroon, S. Shan, W. Saito, G. Broadwater, C. S. Greenberg, and M. W. Dewhirst
Tamoxifen Inhibits Angiogenesis in Estrogen Receptor-negative Animal Models
Clin. Cancer Res., November 1, 2000; 6(11): 4359 - 4364.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
J. Pfeilschifter and I. J. Diel
Osteoporosis Due to Cancer Treatment: Pathogenesis and Management
J. Clin. Oncol., April 7, 2000; 18(7): 1570 - 1593.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
G. Li, Y.-F. Chen, G. L. Greene, S. Oparil, and J. A. Thompson
Estrogen Inhibits Vascular Smooth Muscle Cell-Dependent Adventitial Fibroblast Migration In Vitro
Circulation, October 12, 1999; 100(15): 1639 - 1645.
[Abstract] [Full Text] [PDF]

Home page
 LupusHome page
R T Turner
Mechanical signaling in the development of postmenopausal osteoporosis
Lupus, June 1, 1999; 8(5): 388 - 392.
[Abstract] [PDF]

Home page
 DevelopmentHome page
E Filvaroff, A Erlebacher, J Ye, S. Gitelman, J Lotz, M Heillman, and R Derynck
Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass
Development, January 10, 1999; 126(19): 4267 - 4279.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
P. V. N. Bodine, R. A. Henderson, J. Green, M. Aronow, T. Owen, G. S. Stein, J. B. Lian, and B. S. Komm
Estrogen Receptor-{alpha} Is Developmentally Regulated during Osteoblast Differentiation and Contributes to Selective Responsiveness of Gene Expression
Endocrinology, April 1, 1998; 139(4): 2048 - 2057.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. R. Tau, T. E. Hefferan, K. M. Waters, J. A. Robinson, M. Subramaniam, B. L. Riggs, and T. C. Spelsberg
Estrogen Regulation of a Transforming Growth Factor-{beta} Inducible Early Gene That Inhibits Deoxyribonucleic Acid Synthesis in Human Osteoblasts
Endocrinology, March 1, 1998; 139(3): 1346 - 1353.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. K. Gill, R. T. Turner, T. J. Wronski, and N. H. Bell
Orchiectomy Markedly Reduces the Concentration of the Three Isoforms of Transforming Growth Factor {beta} in Rat Bone, and Reduction Is Prevented by Testosterone
Endocrinology, February 1, 1998; 139(2): 546 - 550.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. h. Sun, M. Mitnick, C. Eielson, G. Q. Yao, I. Paliwal, and K. Insogna
Parathyroid Hormone Increases Circulating Levels of Fibronectin in Vivo: Modulating Effect of Ovariectomy
Endocrinology, September 1, 1997; 138(9): 3918 - 3924.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Luo, C. Labrie, A. Belanger, and F. Labrie
Effect of Dehydroepiandrosterone on Bone Mass, Serum Lipids, and Dimethylbenz(a)anthracene-Induced Mammary Carcinoma in the Rat
Endocrinology, August 1, 1997; 138(8): 3387 - 3394.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. A. Robinson, S. A. Harris, B. L. Riggs, and T. C. Spelsberg
Estrogen Regulation of Human Osteoblastic Cell Proliferation and Differentiation
Endocrinology, July 1, 1997; 138(7): 2919 - 2927.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1991 by The Endocrine Society