| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Endocrinology, Vol 130, 585-591, Copyright © 1992 by Endocrine Society
ARTICLES |
BS Vishwanath, FJ Frey, M Bradbury, MF Dallman and BM Frey
Medizinische Poliklinik, University of Bern, Switzerland.
Clinical and experimental observations revealed that glucocorticoid- deficient states are associated with an enhanced inflammatory response. The antiinflammatory response of pharmacological doses of glucocorticoids has been tentatively attributed to the induction of lipocortin-I. To determine whether glucocorticoid deficiency causes lipocortin-I down-regulation, the expression of lipocortin-I mRNA and protein was quantified in rats with and without adrenalectomy (ADX). The mRNA of lipocortin-I was quantified by polymerase chain reaction, using a constant amount of modified lipocortin-I cDNA transcript as an internal standard. The lipocortin-I mRNA was decreased by 56 +/- 14% in lung tissue of ADX rats. This down-regulation of lipocortin-I mRNA was not due to a nonspecific effect of ADX, since the mRNA levels of other proteins (c-fos, c-myc, c-erbA beta, and metallothionein-II) remained unchanged. The decrease in lipocortin-I mRNA in ADX rats was reflected by a corresponding decrease in tissue (lung, spleen, liver, and kidney) lipocortin-I protein content, as assessed by quantitative Western blot analysis. Thus, ADX causes a decline in lipocortin-I message and protein, an observation compatible with the increased susceptibility to inflammatory reactions in glucocorticoid deficiency.
This article has been cited by other articles:
 |
|
 |
|
  A. Mulla, C. LeRoux, E. Solito, and J. C. Buckingham Correlation between the Antiinflammatory Protein Annexin 1 (Lipocortin 1) and Serum Cortisol in Subjects with Normal and Dysregulated Adrenal Function J. Clin. Endocrinol. Metab., January 1, 2005; 90(1): 557 - 562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Solito, I. A. Romero, S. Marullo, F. Russo-Marie, and B. B. Weksler Annexin 1 Binds to U937 Monocytic Cells and Inhibits Their Adhesion to Microvascular Endothelium: Involvement of the {alpha}4{beta}1 Integrin J. Immunol., August 1, 2000; 165(3): 1573 - 1581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Sapolsky, L. M. Romero, and A. U. Munck How Do Glucocorticoids Influence Stress Responses? Integrating Permissive, Suppressive, Stimulatory, and Preparative Actions Endocr. Rev., February 1, 2000; 21(1): 55 - 89. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. M. FREY, B. F. X. REBER, B. S. VISHWANATH, G. ESCHER, and F. J. FREY Annexin I modulates cell functions by controlling intracellular calcium release FASEB J, December 1, 1999; 13(15): 2235 - 2245. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. C. Christian, A. D. Taylor, R. J. Flower, J. F. Morris, and J. C. Buckingham Characterization and Localization of Lipocortin 1-Binding Sites on Rat Anterior Pituitary Cells by Fluorescence-Activated Cell Analysis/Sorting and Electron Microscopy Endocrinology, December 1, 1997; 138(12): 5341 - 5351. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
