help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, Y. A.
Right arrow Articles by Greenwood, F. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, Y. A.
Right arrow Articles by Greenwood, F. C.

Endocrinology, Vol 130, 1165-1172, Copyright © 1992 by Endocrine Society

ARTICLES

The complementary deoxyribonucleic acid sequence of guinea pig endometrial prorelaxin

YA Lee, GD Bryant-Greenwood, M Mandel and FC Greenwood
Department of Anatomy and Reproductive Biology, University of Hawaii, Honolulu 96822.

The nucleotide sequence of the relaxin gene transcript in the endometrium of the late pregnant guinea pig has been determined. The strategy used was a combination of polymerase chain reaction (PCR) with primers designed from the mRNA sequence of porcine preprorelaxin, rapid amplification of cDNA ends-PCR, and blunt end cloning in M13 mp18. With heterologous primers, a 226-basepair (bp) segment of the guinea pig relaxin gene sequence was obtained and was used to design a guinea pig- specific primer for use with the rapid amplification of cDNA ends-PCR method. The latter allowed completion of the sequence of 336 bp, with a 96-bp overlap. The sequence obtained shows greater homology at both the nucleotide and amino acid levels with porcine and human relaxins H1 and H2 than with rat relaxin, supporting the thesis that the guinea pig is not a rodent. The transcription of the guinea pig endometrial relaxin gene during pregnancy was confirmed by Northern analysis of guinea pig endometrial tissues with a species-specific cDNA probe. The endometrial relaxin gene is transcribed during pregnancy, but not in lactation, consistent with the observed immunostaining for relaxin.


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
T. Klonisch, S. Hombach-Klonisch, C. Froehlich, J. Kauffold, K. Steger, B.G. Steinetz, and B. Fischer
Canine Preprorelaxin: Nucleic Acid Sequence and Localization within the Canine Placenta
Biol Reprod, March 1, 1999; 60(3): 551 - 557.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
T. Klonisch, S. Hombach-Klonisch, C. Froehlich, J. Kauffold, K. Steger, B. Huppertz, and B. Fischer
Nucleic Acid Sequence of Feline Preprorelaxin and Its Localization within the Feline Placenta
Biol Reprod, February 1, 1999; 60(2): 305 - 311.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1992 by The Endocrine Society