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Endocrinology, Vol 130, 2052-2058, Copyright © 1992 by Endocrine Society

ARTICLES

Carbohydrate structures of beta-core fragment of human chorionic gonadotropin isolated from a pregnant individual [published erratum appears in Endocrinology 1992 Sep;131(3):1457]

T Endo, R Nishimura, S Saito, K Kanazawa, K Nomura, M Katsuno, K Shii, K Mukhopadhyay, S Baba and A Kobata
Department of Biochemistry, University of Tokyo, Japan.

Highly purified beta-core fragment was obtained from urine of a pregnant woman with use of an immunoaffinity column. The amino acid sequence of beta-core fragment indicated that it is composed of two polypeptides linked by a disulfide bond. The two polypeptides correspond to the 6-40 and 55-92 portions of hCG beta-subunit. Both Asn13 and Asn30 residues were glycosylated. The N-linked sugar chains of beta-core fragment were quantitatively released as radioactive oligosaccharides by hydrazinolysis, followed by N-acetylation and NaB3H4 reduction. The radioactive oligosaccharides were fractionated by serial lectin column chromatography and Bio-Gel P-4 column chromatography, and their structures were investigated by sequential exoglycosidase digestion and periodate oxidation. The results indicated that they were a mixture of the four oligosaccharides: Man alpha 1---- 6(+/- Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4(+/- Fuc alpha 1----6)GlcNAc. The structural characteristics of the sugar chains of beta-core fragment are quite different from those of the beta-subunit of hCG whose structures were typical biantennary sugar chains containing the Neu5Ac alpha 2----3Gal beta 1----4GlcNAc beta 1----2 group as their outer chains.


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E. S. Jacoby, A. T. Kicman, P. Laidler, and R. K. Iles
Determination of the Glycoforms of Human Chorionic Gonadotropin {beta}-Core Fragment by Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
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C. Liu and L. D. Bowers
Mass spectrometric characterization of nicked fragments of the {beta}-subunit of human chorionic gonadotropin
Clin. Chem., July 1, 1997; 43(7): 1172 - 1181.
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