Endocrinology, Vol 130, 3337-3344, Copyright © 1992 by Endocrine Society

ARTICLES

Testosterone suppresses 8-bromo-adenosine 3',5'-monophosphate and gonadotropin-releasing hormone-stimulated luteinizing hormone subunit synthesis

M Muyan and DM Baldwin
Department of Reproduction, University of California School of Veterinary Medicine, Davis 95616.

The major objective of this study was to determine the effects of testosterone (T) on 8-bromo-cAMP (8-br-cAMP)- and GnRH-stimulated LH subunit polypeptide synthesis and glycosylation in cultured male anterior pituitary cells. The anterior pituitaries from 1-week castrate male rats were enzymatically dispersed and incubated for 48 h in steroid-free medium, followed by a 48-h incubation with or without 10 nM T. The cells were then incubated for 12 h in medium containing [35S]methionine ([35S]Met) and [3H]glucosamine ([3H]Gln) with or without 1 mM 8-br-cAMP or 1 nM GnRH, with or without 10 nM T. Incorporation of radiolabeled precursors into LH subunits was determined by specific immunoprecipitation of the LH dimer with subsequent analysis of the individual LH alpha- and beta-subunits by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. LH content was quantified by RIA (iLH). Both 8-br-cAMP and GnRH stimulated iLH release. T suppressed basal and GnRH-induced iLH secretion, whereas it enhanced iLH release stimulated by 8-br-cAMP. Both 8-br-cAMP and GnRH stimulated total (cell plus medium) [35S]Met and [3H]Gln incorporation into LH alpha and LH beta, and these responses were suppressed by T. Basal [35S]Met incorporation into the LH subunits was inhibited by T, whereas T had no effect on basal levels of [3H]Gln incorporation. Neither T nor GnRH altered [3H]Gln cell uptake or incorporation into total proteins, whereas 8-br-cAMP increased these responses. There were no treatment effects on [35S]Met cell uptake or incorporation into total proteins. These results suggest that 8-br-cAMP, similar to GnRH, stimulates both polypeptide synthesis and glycosylation of the LH alpha- and beta-subunits and that T suppresses these responses to 8-br-cAMP and GnRH in a similar fashion. These data indicate that cAMP is involved in mediating the actions of GnRH on LH subunit biosynthesis and that the inhibition of LH subunit polypeptide synthesis and glycosylation by T involves postreceptor events that are regulated by cAMP-dependent mechanisms.

This article has been cited by other articles:

				H. G. Sutton-Walsh, S. Whelly, and G. A. Cornwall Differential Effects of GnRH and Androgens on Cres mRNA and Protein in Male Mouse Anterior Pituitary Gonadotropes J Androl, November 1, 2006; 27(6): 802 - 815. [Abstract] [Full Text] [PDF]

				D. M. Keenan, P. Y. Takahashi, P. Y. Liu, P. D. Roebuck, A. X. Nehra, A. Iranmanesh, and J. D. Veldhuis An Ensemble Model of the Male Gonadal Axis: Illustrative Application in Aging Men Endocrinology, June 1, 2006; 147(6): 2817 - 2828. [Abstract] [Full Text] [PDF]

				H. Kanasaki, T. Yonehara, Y. Yamada, K. Takahashi, K. Hata, R. Fujiwaki, H. Yamamoto, Y. Takeuchi, K. Fukunaga, E. Miyamoto, et al. Regulation of Gonadotropin {alpha} Subunit Gene Expression by Dopamine D2 Receptor Agonist in Clonal Mouse Gonadotroph {alpha}T3-1 Cells Biol Reprod, October 1, 2002; 67(4): 1218 - 1224. [Abstract] [Full Text] [PDF]

				J. L. Turgeon and D. W. Waring Androgen Modulation of Luteinizing Hormone Secretion by Female Rat Gonadotropes Endocrinology, April 1, 1999; 140(4): 1767 - 1774. [Abstract] [Full Text]

				V. A. Tobin and B. J. Canny The Regulation of Gonadotropin-Releasing Hormone-Induced Calcium Signals in Male Rat Gonadotrophs by Testosterone Is Mediated by Dihydrotestosterone Endocrinology, March 1, 1998; 139(3): 1038 - 1045. [Abstract] [Full Text] [PDF]

				C. Foresta, P. Bordon, M. Rossato, R. Mioni, and J. D. Veldhuis Specific Linkages Among Luteinizing Hormone, Follicle-Stimulating Hormone, and Testosterone Release in the Peripheral Blood and Human Spermatic Vein: Evidence for Both Positive (Feed-Forward) and Negative (Feedback) Within-Axis Regulation J. Clin. Endocrinol. Metab., September 1, 1997; 82(9): 3040 - 3046. [Abstract] [Full Text] [PDF]

				G. Garrel, C. A. McArdle, B. A. Hemmings, and R. Counis Gonadotropin-Releasing Hormone and Pituitary Adenylate Cyclase-Activating Polypeptide Affect Levels of Cyclic Adenosine 3',5'-Monophosphate-Dependent Protein Kinase A (PKA) Subunits in the Clonal Gonadotrope {alpha}T3-1 Cells: Evidence for Cross-Talk between PKA and Protein Kinase C Pathways Endocrinology, June 1, 1997; 138(6): 2259 - 2266. [Abstract] [Full Text] [PDF]