Endocrinology, Vol 131, 166-172, Copyright © 1992 by Endocrine Society

ARTICLES

Identification of somatostatin and gastrin receptors on enterochromaffin-like cells from Mastomys gastric tumors

JC Reubi, B Waser, U Horisberger, F Halter, CJ Soroka, RR Kumar, JR Goldenring and IM Modlin
Sandoz Research Institute, Berne, Switzerland.

Histamine-secreting enterochromaffin-like (ECL) cells of the gastric fundus of the Mastomys can develop into solid ECL cell tumors, either spontaneously or after induction by acid inhibition. We used this tumor tissue to perform in vitro receptor autoradiography for somatostatin (SS), gastrin, and substance-P, using, respectively, [125I]Tyr3- octreotide, [125I]gastrin-17, and [125I]Bolton-Hunter-labeled substance- P as radioligands. A high density of SS receptors was found in the nontumor fundic mucosa, where gastrin receptors were only barely detectable. However, in the group of spontaneously developing ECL cell tumors, a high density of SS and gastrin receptors was observed, homogeneously distributed in the tumor tissue. In addition, the loxtidine-induced ECL cell tumors expressed a high density of SS and gastrin receptors. The receptors were specific for the respective peptide and of high affinity, with a dissociation constant (Kd) of 0.90 nM for SS receptor and 0.87 nM for gastrin receptors. No substance-P receptors were detected on the ECL cell tumors, although they were present in the muscle layers of the Mastomys gastric fundus. These results demonstrate that ECL-derived tumors express receptors for both SS and gastrin. This observation is consistent with the proposal that there is substantial regulation of the histamine-producing ECL cell by SS and gastrin. The presence of gastrin receptors is compatible with a role for gastrin as a trophic factor in ECL cell hyperplasia and neoplasia. The expression of SS receptors may be of diagnostic and therapeutic relevance in the regulation of ECL function and neoplastic transformation.

This article has been cited by other articles:

				J. Nafz, A. Kohler, M. Ohnesorge, I. Nindl, E. Stockfleth, and F. Rosl Persistence of Mastomys natalensis papillomavirus in multiple organs identifies novel targets for infection J. Gen. Virol., October 1, 2007; 88(10): 2670 - 2678. [Abstract] [Full Text] [PDF]

				P. Tomassetti, M. Migliori, G. C. Caletti, P. Fusaroli, R. Corinaldesi, and L. Gullo Treatment of Type II Gastric Carcinoid Tumors with Somatostatin Analogues N. Engl. J. Med., August 24, 2000; 343(8): 551 - 554. [Full Text] [PDF]

				K. Aurang, J. Wang, and K. C. K. Lloyd Somatostatin Inhibition of Acid and Histamine Release by Activation of Somatostatin Receptor Subtype 2 Receptors in Rats J. Pharmacol. Exp. Ther., April 1, 1997; 281(1): 245 - 252. [Abstract] [Full Text]

				H. A. Visser-Wisselaar, C. J. C. van Uffelen, P. M. van Koetsveld, E. G. R. Lichtenauer-Kaligis, A. M. Waaijers, P. Uitterlinden, D. M. Mooy, S. W. J. Lamberts, and L. J. Hofland 17-{beta}-Estradiol-Dependent Regulation of Somatostatin Receptor Subtype Expression in the 7315b Prolactin Secreting Rat Pituitary Tumor in Vitro and in Vivo Endocrinology, March 1, 1997; 138(3): 1180 - 1189. [Abstract] [Full Text] [PDF]