help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maruo, N.
Right arrow Articles by Murota, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maruo, N.
Right arrow Articles by Murota, S.

Endocrinology, Vol 131, 710-714, Copyright © 1992 by Endocrine Society

ARTICLES

IL-6 increases endothelial permeability in vitro

N Maruo, I Morita, M Shirao and S Murota
Section of Physiological Chemistry, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.

The effect of interleukin 6 (IL-6) on endothelial permeability was examined by measuring fluorescein isothiocyanate-labeled albumin flux across an endothelial cell monolayer. Bovine vascular endothelial cells (BVEC) were cultured up to confluency on collagen-coated polycarbonate micropore filters and then the filters were mounted on modified Boyden chambers. Treatment of the BVEC with IL-6 at 100 ng/ml for 21 h caused a remarkable increase in the permeability of fluorescein isothiocyanate- labeled albumin across the endothelial monolayer. This effect of IL-6 was concentration dependent, in the range from 10-200 ng/ml of IL-6. The effect of IL-6 was also time dependent, the maximal level being reached at 21 h from the beginning of the treatment. This stimulatory effect of IL-6 on albumin clearance was completely abolished by the addition of anti-IL-6 antibody. Light microscopic observation of a cross-section of a monolayer showed that the IL-6-induced increase in the permeability was correlated with changes in cell shape and rearrangement of intracellular actin fibers. IL-6 did not show any cytotoxicity toward or growth inhibition of endothelial cells, even at more than 200 ng/ml. The enhancing effect of IL-6 on the increase in the permeability was reversible; when IL-6 was removed by a medium change and the cells were incubated for a further 24 h without IL-6, the permeability was restored to the control level. These results suggest that IL-6 can induce an increase in endothelial permeability in vitro by rearranging actin filaments and by changing the shape of endothelial cells.


This article has been cited by other articles:


Home page
 J. Exp. Med.Home page
K. Luhn, C. P. Simmons, E. Moran, N. T. P. Dung, T. N. B. Chau, N. T. H. Quyen, L. T. T. Thao, T. Van Ngoc, N. M. Dung, B. Wills, et al.
Increased frequencies of CD4+CD25high regulatory T cells in acute dengue infection
J. Exp. Med., May 14, 2007; 204(5): 979 - 985.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Surg. Oncol.Home page
K. Uchide, M. Sakon, H. Ariyoshi, S. Nakamori, M. Tokunaga, and M. Monden
Cancer Cells Cause Vascular Endothelial Cell (vEC) Retraction via 12(S)HETE Secretion; The Possible Role of Cancer Cell Derived Microparticle
Ann. Surg. Oncol., February 1, 2007; 14(2): 862 - 868.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. Villasante, A. Pacheco, A. Ruiz, A. Pellicer, and J. A. Garcia-Velasco
Vascular Endothelial Cadherin Regulates Vascular Permeability: Implications for Ovarian Hyperstimulation Syndrome
J. Clin. Endocrinol. Metab., January 1, 2007; 92(1): 314 - 321.
[Abstract] [Full Text] [PDF]

Home page
 Canadian J. AnesthesiaHome page
P. Farand, M. Hamel, F. Lauzier, G. E. Plante, and O. Lesur
Review article: Organ perfusion/permeability-related effects of norepinephrine and vasopressin in sepsis: [Expose de synthese : Les effets relies a la perfusion et a la permeabilite organique de la norepinephrine et de la vasopressine durant le "sepsis"].
Can J Anesth, September 1, 2006; 53(9): 934 - 946.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
P.-C. Lee, I-C. Ho, and T.-C. Lee
Oxidative Stress Mediates Sodium Arsenite-Induced Expression of Heme Oxygenase-1, Monocyte Chemoattractant Protein-1, and Interleukin-6 in Vascular Smooth Muscle Cells
Toxicol. Sci., May 1, 2005; 85(1): 541 - 550.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Sasamoto, M. Nagino, S. Kobayashi, K. Naruse, Y. Nimura, and M. Sokabe
Mechanotransduction by integrin is essential for IL-6 secretion from endothelial cells in response to uniaxial continuous stretch
Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1012 - C1022.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
T Yanagawa, T Funasaka, S Tsutsumi, H Watanabe, and A Raz
Novel roles of the autocrine motility factor/phosphoglucose isomerase in tumor malignancy
Endocr. Relat. Cancer, December 1, 2004; 11(4): 749 - 759.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C.-W. Ni, H.-J. Hsieh, Y.-J. Chao, and D. L. Wang
Interleukin-6-induced JAK2/STAT3 signaling pathway in endothelial cells is suppressed by hemodynamic flow
Am J Physiol Cell Physiol, September 1, 2004; 287(3): C771 - C780.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. M. Dodd-o, L. E. Welsh, J. D. Salazar, P. L. Walinsky, E. A. Peck, J. G. Shake, D. J. Caparrelli, R. C. Ziegelstein, J. L. Zweier, W. A. Baumgartner, et al.
Effect of NADPH oxidase inhibition on cardiopulmonary bypass-induced lung injury
Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H927 - H936.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. M. Dodd-o, L. E. Welsh, J. D. Salazar, P. L. Walinsky, E. A. Peck, J. G. Shake, D. J. Caparrelli, B. T. Bethea, S. M. Cattaneo, W. A. Baumgartner, et al.
Effect of bronchial artery blood flow on cardiopulmonary bypass-induced lung injury
Am J Physiol Heart Circ Physiol, February 1, 2004; 286(2): H693 - H700.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. U. Gurkan, C. O'Donnell, R. Brower, E. Ruckdeschel, and P. M. Becker
Differential effects of mechanical ventilatory strategy on lung injury and systemic organ inflammation in mice
Am J Physiol Lung Cell Mol Physiol, September 1, 2003; 285(3): L710 - L718.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
C. Lubeseder-Martellato, E. Guenzi, A. Jorg, K. Topolt, E. Naschberger, E. Kremmer, C. Zietz, E. Tschachler, P. Hutzler, M. Schwemmle, et al.
Guanylate-Binding Protein-1 Expression Is Selectively Induced by Inflammatory Cytokines and Is an Activation Marker of Endothelial Cells during Inflammatory Diseases
Am. J. Pathol., November 1, 2002; 161(5): 1749 - 1759.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Y.-W. Lin, K.-J. Wang, H.-Y. Lei, Y.-S. Lin, T.-M. Yeh, H.-S. Liu, C.-C. Liu, and S.-H. Chen
Virus Replication and Cytokine Production in Dengue Virus-Infected Human B Lymphocytes
J. Virol., October 25, 2002; 76(23): 12242 - 12249.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Geimonen, S. Neff, T. Raymond, S. S. Kocer, I. N. Gavrilovskaya, and E. R. Mackow
Pathogenic and nonpathogenic hantaviruses differentially regulate endothelial cell responses
PNAS, October 15, 2002; 99(21): 13837 - 13842.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
C. Albert, N. Garrido, A. Mercader, C.V. Rao, J. Remohi, C. Simon, and A. Pellicer
The role of endothelial cells in the pathogenesis of ovarian hyperstimulation syndrome
Mol. Hum. Reprod., May 1, 2002; 8(5): 409 - 418.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
P. Tassani, H. Schad, C. Winkler, A. Bernhard, U. Ettner, S. L. Braun, G. P. Eising, E. Kochs, R. Lange, and J. A. Richter
Capillary leak syndrome after cardiopulmonary bypass in elective, uncomplicated coronary artery bypass grafting operations: Does it exist?
J. Thorac. Cardiovasc. Surg., April 1, 2002; 123(4): 735 - 741.
[Abstract] [Full Text]

Home page
 JNCI J Natl Cancer InstHome page
R. D'Anna, H. Le Buanec, G. Alessandri, A. Caruso, A. Burny, R. Gallo, J.-F. Zagury, D. Zagury, and P. D'Alessio
Selective Activation of Cervical Microvascular Endothelial Cells by Human Papillomavirus 16-E7 Oncoprotein
J Natl Cancer Inst, December 19, 2001; 93(24): 1843 - 1851.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. A. King, J. S. Marshall, H. Alshurafa, and R. Anderson
Release of Vasoactive Cytokines by Antibody-Enhanced Dengue Virus Infection of a Human Mast Cell/Basophil Line
J. Virol., August 1, 2000; 74(15): 7146 - 7150.
[Abstract] [Full Text]

Home page
 FASEB J.Home page
A. GORNIKIEWICZ, T. SAUTNER, C. BROSTJAN, B. SCHMIERER, R. FÜGGER, E. ROTH, F. MÜHLBACHER, and M. BERGMANN
Catecholamines up-regulate lipopolysaccharide-induced IL-6 production in human microvascular endothelial cells
FASEB J, June 1, 2000; 14(9): 1093 - 1100.
[Abstract] [Full Text]

Home page
 IOVSHome page
P. Gonzalez, D. L. Epstein, and T. Borrás
Genes Upregulated in the Human Trabecular Meshwork in Response to Elevated Intraocular Pressure
Invest. Ophthalmol. Vis. Sci., February 1, 2000; 41(2): 352 - 361.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. H. Ali, S. A. Schlidt, N. S. Chandel, K. L. Hynes, P. T. Schumacker, and B. L. Gewertz
Endothelial permeability and IL-6 production during hypoxia: role of ROS in signal transduction
Am J Physiol Lung Cell Mol Physiol, November 1, 1999; 277(5): L1057 - L1065.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Maret, S. Clamens, I. Delrieu, R. Elhage, J.-F. Arnal, and F. Bayard
Expression of the Interleukin-6 Gene Is Constitutive and Not Regulated by Estrogen in Rat Vascular Smooth Muscle Cells in Culture
Endocrinology, June 1, 1999; 140(6): 2876 - 2882.
[Abstract] [Full Text]

Home page
 J. Clin. Endocrinol. Metab.Home page
O. Serri, P. St-Jacques, M. Sartippour, and G. Renier
Alterations of Monocyte Function in Patients with Growth Hormone (GH) Deficiency: Effect of Substitutive GH Therapy
J. Clin. Endocrinol. Metab., January 1, 1999; 84(1): 58 - 63.
[Abstract] [Full Text]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
W. J. McKinney, R. H. Jaskot, J. H. Richards, D. L. Costa, and K. L. Dreher
Cytokine Mediation of Ozone-induced Pulmonary Adaptation
Am. J. Respir. Cell Mol. Biol., May 1, 1998; 18(5): 696 - 705.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
G.-R. Kleger, P. Bartsch, P. Vock, B. Heilig, L. J. Roberts II, and P. E. Ballmer
Evidence against an increase in capillary permeability in subjects exposed to high altitude
J Appl Physiol, November 1, 1996; 81(5): 1917 - 1923.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
F.-J. Neumann, I. Ott, M. Gawaz, G. Richardt, H. Holzapfel, M. Jochum, and A. Schomig
Cardiac Release of Cytokines and Inflammatory Responses in Acute Myocardial Infarction
Circulation, August 15, 1995; 92(4): 748 - 755.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1992 by The Endocrine Society