Endocrinology, Vol 132, 382-392, Copyright © 1993 by Endocrine Society

ARTICLES

Localization of estrogen receptor messenger ribonucleic acid in rhesus monkey uterus by nonradioactive in situ hybridization with digoxigenin- labeled oligodeoxynucleotides

T Koji and RM Brenner
Department of Anatomy, Nagasaki University School of Medicine, Japan.

Previous immunocytochemical studies indicate that receptor regulation varies in different uterine cell types. In primates, progesterone (P) suppresses estrogen receptor (ER) in glandular epithelial cells in the functionalis, but fails to suppress ER in the glandular epithelial (GE) cells of the basalis. P also fails to suppress ER in the perivascular stromal and smooth muscle cells of the spiral arteries in the functionalis. We used nonradioactive in situ hybridization to determine whether similar cell type differences occur at the ER mRNA level. We used digoxigenin-labeled oligodeoxynucleotides (oligo-DNAs; 45-mer) as probes and detected the hybrids immunocytochemically with horseradish peroxidase-labeled antidigoxigenin antibody. This technique can discriminate between positive and negative cells in closely packed histological associations. In spayed monkeys, most of the GE cells as well as endometrial stromal cells were positive for ER mRNA, while all vascular smooth muscle, endothelium, and perivascular stromal cells were negative. Estradiol treatment for 14 days markedly increased ER mRNA staining in the GE cells, most stromal cells, and the vascular smooth muscle and perivascular stromal cells of spiral arteries in the functionalis. However, in the basalis, these components of the spiral arteries were negative as were the small basal arteries of the basalis. In most positive cells, ER mRNA was not homogeneously distributed in the cytoplasm, but, rather, was concentrated in their perinuclear regions. The GE cells in the basalis had especially intense concentrations of perinuclear signal at their apical poles. After sequential estradiol plus P treatment, the signal was greatly reduced in the GE cells of the functionalis, but not in the GE cells of the basalis or in the vascular smooth muscle or perivascular stromal cells of the spiral arteries of the functionalis. In myometrium, ER mRNA was localized to the perinuclear region of smooth muscle cells, but the staining intensity was not dramatically affected by hormonal manipulation. Unexpectedly, we observed clusters of stromal cells characterized by extremely high positive signals for ER mRNA ("hot cells") at the endometrial/myometrial border and deeper in the connective tissue of the myometrium, although such cells did not express high levels of ER protein. In general, however, the cellular distribution of ER mRNA and its hormonal regulation paralleled those of ER protein.

This article has been cited by other articles:

				S. Ulziibat, K. Ejima, Y. Shibata, Y. Hishikawa, M. Kitajima, A. Fujishita, T. Ishimaru, and T. Koji Identification of estrogen receptor beta-positive intraepithelial lymphocytes and their possible roles in normal and tubal pregnancy oviducts Hum. Reprod., September 1, 2006; 21(9): 2281 - 2289. [Abstract] [Full Text] [PDF]

				K. Shirota, K. Tateishi, T. Koji, Y. Hishikawa, T. Hachisuga, M. Kuroki, and T. Kawarabayashi Early Human Preantral Follicles Have Relaxin and Relaxin Receptor (LGR7), and Relaxin Promotes Their Development J. Clin. Endocrinol. Metab., January 1, 2005; 90(1): 516 - 521. [Abstract] [Full Text] [PDF]

				X. W. Cheng, M. Kuzuya, T. Sasaki, K. Arakawa, S. Kanda, D. Sumi, T. Koike, K. Maeda, N. Tamaya-Mori, G.-P. Shi, et al. Increased Expression of Elastolytic Cysteine Proteases, Cathepsins S and K, in the Neointima of Balloon-Injured Rat Carotid Arteries Am. J. Pathol., January 1, 2004; 164(1): 243 - 251. [Abstract] [Full Text] [PDF]

				H. Wang, H. Eriksson, and L. Sahlin Estrogen Receptors {alpha} and {beta} in the Female Reproductive Tract of the Rat During the Estrous Cycle Biol Reprod, November 1, 2000; 63(5): 1331 - 1340. [Abstract] [Full Text]

				S. Matsuzaki, T. Fukaya, T. Suzuki, T. Murakami, H. Sasano, and A. Yajima Oestrogen receptor {alpha} and ß mRNA expression in human endometrium throughout the menstrual cycle Mol. Hum. Reprod., June 1, 1999; 5(6): 559 - 564. [Abstract] [Full Text] [PDF]

				Y. Nakamura, T. Usui, H. Mizuta, H. Murabe, S. Muro, M. Suda, K. Tanaka, I. Tanaka, A. Shimatsu, and K. Nakao Characterization of Prophet of Pit-1 Gene Expression in Normal Pituitary and Pituitary Adenomas in Humans J. Clin. Endocrinol. Metab., April 1, 1999; 84(4): 1414 - 1419. [Abstract] [Full Text]

				K. Ukena, C. Kohchi, and K. Tsutsui Expression and Activity of 3{beta}-Hydroxysteroid Dehydrogenase/{Delta}5-{Delta}4-Isomerase in the Rat Purkinje Neuron during Neonatal Life Endocrinology, February 1, 1999; 140(2): 805 - 813. [Abstract] [Full Text]

				A. Orimo, S. Inoue, K. Ikeda, S. Noji, and M. Muramatsu Molecular Cloning, Structure, and Expression of Mouse Estrogen-responsive Finger Protein Efp J. Biol. Chem., October 13, 1995; 270(41): 24406 - 24413. [Abstract] [Full Text] [PDF]

				S. M. Hyder, Z. Nawaz, C. Chiappetta, K. Yokoyama, and G. M. Stancel The Protooncogene c- jun Contains an Unusual Estrogen-inducible Enhancer within the Coding Sequence J. Biol. Chem., April 14, 1995; 270(15): 8506 - 8513. [Abstract] [Full Text] [PDF]

				B Risek, F. Klier, A Phillips, D. Hahn, and N. Gilula Gap junction regulation in the uterus and ovaries of immature rats by estrogen and progesterone J. Cell Sci., January 3, 1995; 108(3): 1017 - 1032. [Abstract] [PDF]