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Endocrinology, Vol 133, 166-170, Copyright © 1993 by Endocrine Society

ARTICLES

Cross-talk between Ca2+ influx and phosphoinositide hydrolysis by extracellular adenosine triphosphate in rat PC12 pheochromocytoma cells

O Kozawa, M Miwa and H Tokuda
Department of Biochemistry, Institute for Developmental Research, Aichi Prefectural Colony (O.K.), Japan.

In rat PC12 pheochromocytoma cells, extracellular ATP stimulated 45Ca2+ influx even in the presence of nifedipine, a Ca2+ antagonist that inhibits the voltage-dependent L-type Ca2+ channel, and stimulated the formation of inositol trisphosphate (IP3). The effect of ATP on 45Ca2+ influx was more potent than that on the formation of IP3 at a dose lower than 0.1 mM. 12-O-Tetradecanoylphorbol-13-acetate, a protein kinase-C (PKC)-activating phorbol ester, which by itself had little effect on 45Ca2+ influx, significantly reduced the ATP-induced 45Ca2+ influx in a dose-dependent manner in the range between 1 nM and 0.1 microM. However, 4 alpha-phorbol 12, 13-didecanoate, a PKC- nonactivating phorbol ester, had little effect on the 45Ca2+ influx induced by ATP. Staurosporine, an inhibitor for PKC, significantly enhanced the ATP-induced 45Ca2+ influx. Pertussis toxin inhibited the ATP-induced formation of IP3 in a dose-dependent manner in the range between 0.1 ng/ml and 1 microgram/ml. On the other hand, pertussis toxin significantly enhanced the ATP-induced 45Ca2+ influx. These results strongly suggest that extracellular ATP-induced Ca2+ influx is autoregulated due to the activation of PKC resulting from pertussis toxin-sensitive GTP-binding protein-coupled phosphoinositide hydrolysis in PC12 pheochromocytoma cells.


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