Endocrinology, Vol 133, 29-32, Copyright © 1993 by Endocrine Society

ARTICLES

A role for neuropeptide-Y, dynorphin, and noradrenaline in the central control of food intake after food deprivation

PD Lambert, JP Wilding, AA al-Dokhayel, C Bohuon, E Comoy, SG Gilbey and SR Bloom
Division of Endocrinology and Metabolism, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.

A marked increase in food intake is observed in the rat after central injection of neuropeptide-Y (NPY), dynorphin, or noradrenaline (NA). Levels of both NPY and dynorphin are increased in the hypothalamus of food-deprived rats. The aim of this study was to explore the role of NPY, dynorphin, and NA in the central control of feeding after a period of food deprivation. We have investigated the effect of intracerebroventricular injection of a monoclonal antibody to NPY (NPYAb), a potent and selective kappa-opioid receptor antagonist norbinaltorphimine (norBNI), and the alpha-adrenergic antagonist phentolamine on fast-induced food intake. In animals provided with food after a 24-h fast, NPYAb given 10 min before presentation of food reduced food intake by 30% (P < 0.01) compared to that of animals pretreated with an antibody to chloroquine. A similar (34%; P < 0.05) reduction in fast-induced feeding occurred after pretreatment with norBNI. If norBNI was given together with NPYAb, then a reduction of 51% (P < 0.05) was observed. Pretreatment with phentolamine reduced fast-induced food intake by 39% (P < 0.05), with no evidence of an additive effect when phentolamine was given together with NPYAb. These data would support a role for endogenous NPY, dynorphin, and NA in the mediation of fast-induced feeding. NPY would seem to act independently of dynorphin, but through the same mechanism as NA.

This article has been cited by other articles:

				A. Sainsbury, S. Lin, K. McNamara, K. Slack, R. Enriquez, N. J. Lee, D. Boey, G. A. Smythe, C. Schwarzer, P. Baldock, et al. Dynorphin Knockout Reduces Fat Mass and Increases Weight Loss during Fasting in Mice Mol. Endocrinol., July 1, 2007; 21(7): 1722 - 1735. [Abstract] [Full Text] [PDF]

				A. Inui Transgenic Approach to the Study of Body Weight Regulation Pharmacol. Rev., March 1, 2000; 52(1): 35 - 62. [Abstract] [Full Text] [PDF]

				D. Gayle, S. E. Ilyin, and C. R. Plata-Salaman Feeding status and bacterial LPS-induced cytokine and neuropeptide gene expression in hypothalamus Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1999; 277(4): R1188 - R1195. [Abstract] [Full Text] [PDF]

				S. P. Kalra, M. G. Dube, S. Pu, B. Xu, T. L. Horvath, and P. S. Kalra Interacting Appetite-Regulating Pathways in the Hypothalamic Regulation of Body Weight Endocr. Rev., February 1, 1999; 20(1): 68 - 100. [Abstract] [Full Text]

				K. Meeran, D. O’Shea, C. M. B. Edwards, M. D. Turton, M. M. Heath, I. Gunn, S. Abusnana, M. Rossi, C. J. Small, A. P. Goldstone, et al. Repeated Intracerebroventricular Administration of Glucagon-Like Peptide-1-(7-36) Amide or Exendin-(9-39) Alters Body Weight in the Rat Endocrinology, January 1, 1999; 140(1): 244 - 250. [Abstract] [Full Text]

				C. M. Kotz, J. E. Briggs, M. K. Grace, A. S. Levine, and C. J. Billington Divergence of the feeding and thermogenic pathways influenced by NPY in the hypothalamic PVN of the rat Am J Physiol Regulatory Integrative Comp Physiol, August 1, 1998; 275(2): R471 - R477. [Abstract] [Full Text] [PDF]

				A. V. Turnbull and C. L. Rivier Intracerebroventricular Passive Immunization. II. Intracerebroventricular Infusion of Neuropeptide Antisera Can Inhibit Neuropeptide Signaling in Peripheral Tissues Endocrinology, January 1, 1998; 139(1): 128 - 136. [Abstract] [Full Text] [PDF]

				D. O'Shea, D. G. A. Morgan, K. Meeran, C. M. B. Edwards, M. D. Turton, S. J. Choi, M. M. Heath, I. Gunn, G. M. Taylor, J. K. Howard, et al. Neuropeptide Y Induced Feeding in the Rat Is Mediated by a Novel Receptor Endocrinology, January 1, 1997; 138(1): 196 - 202. [Abstract] [Full Text] [PDF]