help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vanderhyden, B. C.
Right arrow Articles by Morley, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vanderhyden, B. C.
Right arrow Articles by Morley, P.

Endocrinology, Vol 133, 423-426, Copyright © 1993 by Endocrine Society

ARTICLES

Mouse oocytes regulate granulosa cell steroidogenesis

BC Vanderhyden, JN Cohen and P Morley
Department of Medicine, University of Ottawa, Ontario, Canada.

Recent studies have demonstrated a critical role for the oocyte in proliferation and differentiation of granulosa cells and expansion of the cumulus oophorus in vitro. The purpose of this study was to determine if steroid production by cumulus granulosa cells was also modulated by oocytes. Mouse oocyte-cumulus cell complexes (intact) and complexes from which the oocytes were removed microsurgically (oocytectomized; OOX) were cultured for 24 h in the presence or absence of follicle-stimulating hormone (FSH; 150 ng/ml), testosterone (T; 5 x 10(-7) M) or both. Oocytectomy had no effect on the ability of cumulus cells to produce progesterone or estradiol in control cultures or in response to T. However, OOX complexes produced 17- and 36-fold more progesterone than intact complexes when cultured in the presence of FSH or FSH+T, respectively. Oocyte-conditioned medium (maximum 1 oocyte/2 microliters) had no effect on progesterone production by intact cumulus complexes, but reduced the progesterone production by OOX complexes by 75%. This inhibition was directly proportional to the number of oocytes used to condition the medium. Oocytectomy caused a slight decrease (29%) in estradiol production by complexes in the presence of FSH and T; however, OOX complexes in oocyte-conditioned medium produced almost twice as much estradiol as complexes in unconditioned medium. These results indicate that mouse oocytes secrete a factor(s) that inhibits progesterone and stimulates estradiol production by cumulus granulosa cells.


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
R. B. Gilchrist, M. Lane, and J. G. Thompson
Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality
Hum. Reprod. Update, March 1, 2008; 14(2): 159 - 177.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Miyoshi, F. Otsuka, K. Inagaki, H. Otani, M. Takeda, J. Suzuki, J. Goto, T. Ogura, and H. Makino
Differential Regulation of Steroidogenesis by Bone Morphogenetic Proteins in Granulosa Cells: Involvement of Extracellularly Regulated Kinase Signaling and Oocyte Actions in Follicle-Stimulating Hormone-Induced Estrogen Production
Endocrinology, January 1, 2007; 148(1): 337 - 345.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y.-Q. Su, M. Nyegaard, M. T. Overgaard, J. Qiao, and L. C. Giudice
Participation of Mitogen-Activated Protein Kinase in Luteinizing Hormone-Induced Differential Regulation of Steroidogenesis and Steroidogenic Gene Expression in Mural and Cumulus Granulosa Cells of Mouse Preovulatory Follicles
Biol Reprod, December 1, 2006; 75(6): 859 - 867.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. B. Gilchrist, L. J. Ritter, S. Myllymaa, N. Kaivo-Oja, R. A. Dragovic, T. E. Hickey, O. Ritvos, and D. G. Mottershead
Molecular basis of oocyte-paracrine signalling that promotes granulosa cell proliferation
J. Cell Sci., September 15, 2006; 119(18): 3811 - 3821.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. A. Pangas, X. Li, E. J. Robertson, and M. M. Matzuk
Premature Luteinization and Cumulus Cell Defects in Ovarian-Specific Smad4 Knockout Mice
Mol. Endocrinol., June 1, 2006; 20(6): 1406 - 1422.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. J. Guigon and S. Magre
Contribution of Germ Cells to the Differentiation and Maturation of the Ovary: Insights from Models of Germ Cell Depletion
Biol Reprod, March 1, 2006; 74(3): 450 - 458.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
T.E. Hickey, D.L. Marrocco, F. Amato, L.J. Ritter, R.J. Norman, R.B. Gilchrist, and D.T. Armstrong
Androgens Augment the Mitogenic Effects of Oocyte-Secreted Factors and Growth Differentiation Factor 9 on Porcine Granulosa Cells
Biol Reprod, October 1, 2005; 73(4): 825 - 832.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
D Nogueira, R Cortvrindt, B Everaerdt, and J Smitz
Effects of long-term in vitro exposure to phosphodiesterase type-3 inhibitors on follicle and oocyte development
Reproduction, August 1, 2005; 130(2): 177 - 186.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
F. Otsuka, R. K. Moore, X. Wang, S. Sharma, T. Miyoshi, and S. Shimasaki
Essential Role of the Oocyte in Estrogen Amplification of Follicle-Stimulating Hormone Signaling in Granulosa Cells
Endocrinology, August 1, 2005; 146(8): 3362 - 3367.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. A. Dragovic, L. J. Ritter, S. J. Schulz, F. Amato, D. T. Armstrong, and R. B. Gilchrist
Role of Oocyte-Secreted Growth Differentiation Factor 9 in the Regulation of Mouse Cumulus Expansion
Endocrinology, June 1, 2005; 146(6): 2798 - 2806.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. Prochazka, L. Nemcova, E. Nagyova, and J. Kanka
Expression of Growth Differentiation Factor 9 Messenger RNA in Porcine Growing and Preovulatory Ovarian Follicles
Biol Reprod, October 1, 2004; 71(4): 1290 - 1295.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R.B. Gilchrist, L.J. Ritter, M. Cranfield, L.A. Jeffery, F. Amato, S.J. Scott, S. Myllymaa, N. Kaivo-Oja, H. Lankinen, D.G. Mottershead, et al.
Immunoneutralization of Growth Differentiation Factor 9 Reveals It Partially Accounts for Mouse Oocyte Mitogenic Activity
Biol Reprod, September 1, 2004; 71(3): 732 - 739.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y.-L. Kang, H. Li, W.-H. Chen, Y.-S. Tzeng, Y.-L. Lai, and H. M. Hsieh-Li
A Novel PEPP Homeobox Gene, TOX, Is Highly Glutamic Acid Rich and Specifically Expressed in Murine Testis and Ovary
Biol Reprod, March 1, 2004; 70(3): 828 - 836.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. C. SAEZ, V. M. BERTHOUD, M. C. BRANES, A. D. MARTINEZ, and E. C. BEYER
Plasma Membrane Channels Formed by Connexins: Their Regulation and Functions
Physiol Rev, October 1, 2003; 83(4): 1359 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Glister, N. P. Groome, and P. G. Knight
Oocyte-Mediated Suppression of Follicle-Stimulating Hormone- and Insulin-Like Growth Factor-Induced Secretion of Steroids and Inhibin-Related Proteins by Bovine Granulosa Cells In Vitro: Possible Role of Transforming Growth Factor {alpha}
Biol Reprod, March 1, 2003; 68(3): 758 - 765.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. Prochazka, P. Kalab, and E. Nagyova
Epidermal Growth Factor-Receptor Tyrosine Kinase Activity Regulates Expansion of Porcine Oocyte-Cumulus Cell Complexes In Vitro
Biol Reprod, March 1, 2003; 68(3): 797 - 803.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
N. Yamamoto, L. K. Christenson, J. M. MCAllister, and J. F. Strauss III
Growth Differentiation Factor-9 Inhibits 3'5'-Adenosine Monophosphate-Stimulated Steroidogenesis in Human Granulosa and Theca Cells
J. Clin. Endocrinol. Metab., June 1, 2002; 87(6): 2849 - 2856.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
I. Granot, E. Bechor, A. Barash, and N. Dekel
Connexin43 in Rat Oocytes: Developmental Modulation of Its Phosphorylation
Biol Reprod, March 1, 2002; 66(3): 568 - 573.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. H.C. Yao and J. M. Bahr
Germinal Disc-Derived Epidermal Growth Factor: A Paracrine Factor to Stimulate Proliferation of Granulosa Cells
Biol Reprod, January 1, 2001; 64(1): 390 - 395.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
R. Li, R. J. Norman, D. T. Armstrong, and R. B. Gilchrist
Oocyte-Secreted Factor(s) Determine Functional Differences Between Bovine Mural Granulosa Cells and Cumulus Cells
Biol Reprod, September 1, 2000; 63(3): 839 - 845.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
J. V. Planas, J. Athos, F. W. Goetz, and P. Swanson
Regulation of Ovarian Steroidogenesis In Vitro by Follicle-Stimulating Hormone and Luteinizing Hormone During Sexual Maturation in Salmonid Fish
Biol Reprod, May 1, 2000; 62(5): 1262 - 1269.
[Abstract] [Full Text]

Home page
 Hum ReprodHome page
A. Dhawan, M.-C. Leveille, and B.C. Vanderhyden
Inhibition by human embryos of mouse granulosa cell progesterone production: development of a sensitive bioassay
Hum. Reprod., April 1, 2000; 15(4): 917 - 924.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. J. Eppig and K. Wigglesworth
Development of Mouse and Rat Oocytes in Chimeric Reaggregated Ovaries after Interspecific Exchange of Somatic and Germ Cell Components
Biol Reprod, April 1, 2000; 63(4): 1014 - 1023.
[Abstract] [Full Text]

Home page
 Endocr. Rev.Home page
J. F. Couse and K. S. Korach
Estrogen Receptor Null Mice: What Have We Learned and Where Will They Lead Us?
Endocr. Rev., June 1, 1999; 20(3): 358 - 417.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
J. A. Elvin, A. T. Clark, P. Wang, N. M. Wolfman, and M. M. Matzuk
Paracrine Actions Of Growth Differentiation Factor-9 in the Mammalian Ovary
Mol. Endocrinol., June 1, 1999; 13(6): 1035 - 1048.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
B. C. Vanderhyden and E. A. Macdonald
Mouse Oocytes Regulate Granulosa Cell Steroidogenesis Throughout Follicular Development
Biol Reprod, December 1, 1998; 59(6): 1296 - 1301.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
H. Hung-Chang Yao, K. K. Volentine, and J. M. Bahr
Destruction of the Germinal Disc Region of an Immature Preovulatory Chicken Follicle Induces Atresia and Apoptosis
Biol Reprod, July 1, 1998; 59(3): 516 - 521.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
K. K. Volentine, H. H.-C. Yao, and J. M. Bahr
Epidermal Growth Factor in the Germinal Disc and Its Potential Role in Follicular Development in the Chicken
Biol Reprod, July 1, 1998; 59(3): 522 - 526.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
M. Li, E. G. Karakji, R. Xing, J. N. Fryer, J. A. Carnegie, S. A. Rabbani, and B. K. Tsang
Expression of Urokinase-Type Plasminogen Activator and Its Receptor during Ovarian Follicular Development
Endocrinology, July 1, 1997; 138(7): 2790 - 2799.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. A. Elvin, C. Yan, and M. M. Matzuk
Growth differentiation factor-9 stimulates progesterone synthesis in granulosa cells via a prostaglandin E2/EP2 receptor pathway
PNAS, August 29, 2000; 97(18): 10288 - 10293.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1993 by The Endocrine Society