Endocrinology, Vol 133, 1669-1677, Copyright © 1993 by Endocrine Society

ARTICLES

Bovine adrenal glomerulosa and fasciculata cells exhibit 28.5- kilodalton proteins sensitive to angiotensin, other agonists, and atrial natriuretic peptide

ME Elliott, TL Goodfriend and CR Jefcoate
William S. Middleton Memorial Veterans Hospital, Madison Wisconsin 53705.

Protein synthesis by bovine adrenal glomerulosa and fasciculata cells in response to various modulators of steroid synthesis was examined using [35S]methionine labeling and two-dimensional gel electrophoresis. Both cell types responded to steroidogenic stimuli with rapid changes in a family of 28- to 30-kilodalton (kDa) proteins similar to those described in rat fasciculata by Epstein and Orme-Johnson. In glomerulosa, angiotensin-II (AII), potassium, and (Bu)2cAMP stimulated the appearance of two 28.5-kDa proteins (no. 3 and 4) with pI values of 6.44 and 6.33 and decreased labeling of two other 28.5-kDa proteins (no. 1 and 2) with pI values of 6.9 and 6.59. The rank order of potency on aldosterone synthesis and that on proteins 1-4 were the same: (Bu)2cAMP > AII > potassium. Atrial natriuretic peptide blocked the effects of AII on all four proteins and on aldosterone synthesis. Adrenal secretagogues also affected labeling of four slightly larger (30 kDa) proteins (no. 5-8). Corresponding proteins in each quartet are separated by the same difference in isoelectric points. These eight proteins may represent a core protein systematically modified in a number of ways. Aldosterone synthesis in glomerulosa, like glucocorticoid synthesis in fasciculata, requires ongoing protein synthesis. The 28- to 30-kDa proteins increased by steroidogenic stimuli in both cells and decreased by atrial natriuretic peptide in glomerulosa may be the proteins whose synthesis is crucial to acute control of steroidogenesis. Our results indicate that these proteins are made in response to calcium- or calcium/phosphoinositide-dependent mechanisms as well as by cAMP.

This article has been cited by other articles:

				A. SPAT and L. HUNYADY Control of Aldosterone Secretion: A Model for Convergence in Cellular Signaling Pathways Physiol Rev, April 1, 2004; 84(2): 489 - 539. [Abstract] [Full Text] [PDF]

				J. Li, R. E. Feltzer, K. L. Dawson, E. A. Hudson, and B. J. Clark Janus Kinase 2 and Calcium Are Required for Angiotensin II-dependent Activation of Steroidogenic Acute Regulatory Protein Transcription in H295R Human Adrenocortical Cells J. Biol. Chem., December 26, 2003; 278(52): 52355 - 52362. [Abstract] [Full Text] [PDF]

				B. D. Gaylinn, V. I. DeAlmeida, C. E. Lyons Jr., K. C. Wu, K. E. Mayo, and M. O. Thorner The Mutant Growth Hormone-Releasing Hormone (GHRH) Receptor of the Little Mouse Does Not Bind GHRH Endocrinology, November 1, 1999; 140(11): 5066 - 5074. [Abstract] [Full Text]

				B. J. Clark and R. Combs Angiotensin II and Cyclic Adenosine 3',5'-Monophosphate Induce Human Steroidogenic Acute Regulatory Protein Transcription through a Common Steroidogenic Factor-1 Element Endocrinology, October 1, 1999; 140(10): 4390 - 4398. [Abstract] [Full Text]

				B. Peters, S. Clausmeyer, N. Obermüller, A. Woyth, B. Kränzlin, N. Gretz, and J. Peters Specific Regulation of StAR Expression in the Rat Adrenal Zona Glomerulosa: an In Situ Hybridization Study J. Histochem. Cytochem., November 1, 1998; 46(11): 1215 - 1222. [Abstract] [Full Text]

				N. Cherradi, Y. Brandenburger, M. F. Rossier, M. B. Vallotton, D. M. Stocco, and A. M. Capponi Atrial Natriuretic Peptide Inhibits Calcium-Induced Steroidogenic Acute Regulatory Protein Gene Transcription in Adrenal Glomerulosa Cells Mol. Endocrinol., July 1, 1998; 12(7): 962 - 972. [Abstract] [Full Text]

				N. Ariyoshi, Y.-C. Kim, I. Artemenko, K. K. Bhattacharyya, and C. R. Jefcoate Characterization of the Rat Star Gene That Encodes the Predominant 3.5-Kilobase Pair mRNA. ACTH STIMULATION OF ADRENAL STEROIDS IN VIVO PRECEDES ELEVATION OF Star mRNA AND PROTEIN J. Biol. Chem., March 27, 1998; 273(13): 7610 - 7619. [Abstract] [Full Text] [PDF]

				H. Amri, K. Drieu, and V. Papadopoulos Ex Vivo Regulation of Adrenal Cortical Cell Steroid and Protein Synthesis, in Response to Adrenocorticotropic Hormone Stimulation, by the Ginkgo biloba Extract EGb 761 and Isolated Ginkgolide B Endocrinology, December 1, 1997; 138(12): 5415 - 5426. [Abstract] [Full Text] [PDF]

				A. Amsterdam and N. Selvaraj Control of Differentiation, Transformation, and Apoptosis in Granulosa Cells by Oncogenes, Oncoviruses, and Tumor Suppressor Genes Endocr. Rev., August 1, 1997; 18(4): 435 - 461. [Abstract] [Full Text] [PDF]

				M. E. Elliott, T. L. Goodfriend, D. L. Ball, and C. R. Jefcoate Angiotensin-Responsive Adrenal Glomerulosa Cell Proteins: Characterization by Protease Mapping, Species Comparison, and Specific Angiotensin Receptor Antagonists Endocrinology, June 1, 1997; 138(6): 2530 - 2536. [Abstract] [Full Text] [PDF]

				N. Cherradi, M. F. Rossier, M. B. Vallotton, R. Timberg, I. Friedberg, J. Orly, X. J. Wang, D. M. Stocco, and A. M. Capponi Submitochondrial Distribution of Three Key Steroidogenic Proteins (Steroidogenic Acute Regulatory Protein and Cytochrome P450scc and 3beta -Hydroxysteroid Dehydrogenase Isomerase Enzymes) upon Stimulation by Intracellular Calcium in Adrenal Glomerulosa Cells J. Biol. Chem., March 21, 1997; 272(12): 7899 - 7907. [Abstract] [Full Text] [PDF]

				N. Pilon, I. Daneau, C. Brisson, J.-F. Ethier, J. G. Lussier, and D. W. Silversides Porcine and Bovine Steroidogenic Acute Regulatory Protein (StAR) Gene Expression during Gestation Endocrinology, March 1, 1997; 138(3): 1085 - 1091. [Abstract] [Full Text] [PDF]

				N. Cherradi, M. F. Rossier, M. B. Vallotton, and A. M. Capponi Calcium Stimulates Intramitochondrial Cholesterol Transfer in Bovine Adrenal Glomerulosa Cells J. Biol. Chem., October 18, 1996; 271(42): 25971 - 25975. [Abstract] [Full Text] [PDF]