Endocrinology, Vol 133, 1715-1723, Copyright © 1993 by Endocrine Society

ARTICLES

Pretranslational regulation of type I 5'-deiodinase by thyroid hormones and in fasted and diabetic rats

BA O'Mara, W Dittrich, TJ Lauterio and DL St Germain
Department of Medicine, Dartmouth Medical School Lebanon, New Hampshire 03756.

In both humans and experimental animals, nutritional deprivation and systemic illness are associated with decreases in circulating T3 levels, T3 production, and type I 5'-deiodinase (5'DI) activity. In order to assess the regulation of 5'DI messenger RNA (mRNA) in these conditions, a solution hybridization assay was developed which utilized a complementary (cDNA) encompassing 92% of the 5'DI coding region. The administration of T3 to hypothyroid rats induced a 50-fold increase in 5'DI mRNA levels that preceded by 12 h a similar increase in 5'DI activity. Fasting for 48-72 h, but not 24 h, was associated with an approximate 50% decrease in 5'DI mRNA levels in liver, which preceded a decrease of similar magnitude in 5'DI activity. Fasting in hyperthyroid rats did not lower hepatic 5'DI mRNA levels or activity, nor did fasting attenuate the stimulatory effects of T3 administration on these parameters. In diabetic animals 5'DI mRNA levels and activity were markedly reduced. These results demonstrate that regulation of 5'DI by thyroid hormones and in fasting and diabetes mellitus is exerted principally at the pretranslational level. The relatively late occurrence of decreases in 5'DI mRNA levels and activity observed in nutritional deprivation suggests that these changes are secondary to, rather than causative of, the decreased serum and tissue T3 levels noted in these conditions.

This article has been cited by other articles:

				Y. Debaveye, B. Ellger, L. Mebis, T. J. Visser, V. M. Darras, and G. Van den Berghe Effects of Substitution and High-Dose Thyroid Hormone Therapy on Deiodination, Sulfoconjugation, and Tissue Thyroid Hormone Levels in Prolonged Critically Ill Rabbits Endocrinology, August 1, 2008; 149(8): 4218 - 4228. [Abstract] [Full Text] [PDF]

				R. L. Araujo, B. M. de Andrade, A. S. P. de Figueiredo, M. L. da Silva, M. P. Marassi, V. dos Santos Pereira, E. Bouskela, and D. P Carvalho Low replacement doses of thyroxine during food restriction restores type 1 deiodinase activity in rats and promotes body protein loss J. Endocrinol., July 1, 2008; 198(1): 119 - 125. [Abstract] [Full Text] [PDF]

				M. H. A. Kester, G. G. J. M. Kuiper, R. Versteeg, and T. J. Visser Regulation of Type III Iodothyronine Deiodinase Expression in Human Cell Lines Endocrinology, December 1, 2006; 147(12): 5845 - 5854. [Abstract] [Full Text] [PDF]

				Y. Debaveye, B. Ellger, L. Mebis, E. Van Herck, W. Coopmans, V. Darras, and G. Van den Berghe Tissue Deiodinase Activity during Prolonged Critical Illness: Effects of Exogenous Thyrotropin-Releasing Hormone and Its Combination with Growth Hormone-Releasing Peptide-2 Endocrinology, December 1, 2005; 146(12): 5604 - 5611. [Abstract] [Full Text] [PDF]

				A. Boelen, J. Kwakkel, A. Alkemade, R. Renckens, E. Kaptein, G. Kuiper, W. M. Wiersinga, and T. J. Visser Induction of Type 3 Deiodinase Activity in Inflammatory Cells of Mice with Chronic Local Inflammation Endocrinology, December 1, 2005; 146(12): 5128 - 5134. [Abstract] [Full Text] [PDF]

				R. Martinez-deMena and M.-J. Obregon Insulin increases the adrenergic stimulation of 5' deiodinase activity and mRNA expression in rat brown adipocytes; role of MAPK and PI3K J. Mol. Endocrinol., February 1, 2005; 34(1): 139 - 151. [Abstract] [Full Text] [PDF]

				F. W. J. S. Wassen, W. Klootwijk, E. Kaptein, D. J. Duncker, T. J. Visser, and G. G. J. M. Kuiper Characteristics and Thyroid State-Dependent Regulation of Iodothyronine Deiodinases in Pigs Endocrinology, September 1, 2004; 145(9): 4251 - 4263. [Abstract] [Full Text] [PDF]

				J. Slone Wilcoxon and E. E. Redei Prenatal programming of adult thyroid function by alcohol and thyroid hormones Am J Physiol Endocrinol Metab, August 1, 2004; 287(2): E318 - E326. [Abstract] [Full Text] [PDF]

				S. Friedrichsen, S. Christ, H. Heuer, M. K. H. Schafer, A. Mansouri, K. Bauer, and T. J. Visser Regulation of Iodothyronine Deiodinases in the Pax8-/- Mouse Model of Congenital Hypothyroidism Endocrinology, March 1, 2003; 144(3): 777 - 784. [Abstract] [Full Text] [PDF]

				R. P. Peeters, E. C. H. Friesema, R. Docter, G. Hennemann, and T. J. Visser Effects of thyroid state on the expression of hepatic thyroid hormone transporters in rats Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1232 - E1238. [Abstract] [Full Text] [PDF]

				A. C. Bianco, D. Salvatore, B. Gereben, M. J. Berry, and P. R. Larsen Biochemistry, Cellular and Molecular Biology, and Physiological Roles of the Iodothyronine Selenodeiodinases Endocr. Rev., February 1, 2002; 23(1): 38 - 89. [Abstract] [Full Text] [PDF]

				S. Pallud, M. Ramaugé, J.-M. Gavaret, A.-M. Lennon, N. Munsch, D. L. St. Germain, M. Pierre, and F. Courtin Regulation of Type 3 Iodothyronine Deiodinase Expression in Cultured Rat Astrocytes: Role of the Erk Cascade Endocrinology, June 1, 1999; 140(6): 2917 - 2923. [Abstract] [Full Text]

				S. Van der Geyten, J. P. Sanders, E. Kaptein, V. M. Darras, E. R. Kuhn, J. L. Leonard, and T. J. Visser Expression of Chicken Hepatic Type I and Type III Iodothyronine Deiodinases during Embryonic Development Endocrinology, December 1, 1997; 138(12): 5144 - 5152. [Abstract] [Full Text] [PDF]

				H. F. Escobar-Morreale, M. J. Obregon, A. Hernandez, F. Escobar del Rey, and G. Morreale de Escobar Regulation of Iodothyronine Deiodinase Activity as Studied in Thyroidectomized Rats Infused with Thyroxine or Triiodothyronine Endocrinology, June 1, 1997; 138(6): 2559 - 2568. [Abstract] [Full Text] [PDF]

				R. Calvo, G. Morreale de Escobar, F. Escobar del Rey, and M.-J. Obregon Maternal Nonthyroidal Illness and Fetal Thyroid Hormone Status, as Studied in the Streptozotocin-Induced Diabetes Mellitus Rat Model Endocrinology, March 1, 1997; 138(3): 1159 - 1169. [Abstract] [Full Text] [PDF]

				C. Valverde-R, W. Croteau, G. J. LaFleur Jr., A. Orozco, and D. L. St. Germain Cloning and Expression of a 5'-Iodothyronine Deiodinase from the Liver of Fundulus heteroclitus Endocrinology, February 1, 1997; 138(2): 642 - 648. [Abstract] [Full Text] [PDF]

				SuzanneB. Brown, M. Maloney, and WilliamB. Kinlaw "Spot 14" Protein Functions at the Pretranslational Level in the Regulation of Hepatic Metabolism by Thyroid Hormone and Glucose J. Biol. Chem., January 24, 1997; 272(4): 2163 - 2166. [Abstract] [Full Text] [PDF]

				W. Croteau, S. L. Whittemore, M. J. Schneider, and D. L. St. Germain Cloning and Expression of a cDNA for a Mammalian Type III Iodothyronine Deiodinase J. Biol. Chem., July 14, 1995; 270(28): 16569 - 16575. [Abstract] [Full Text] [PDF]