help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rao, Y. P.
Right arrow Articles by Buckley, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rao, Y. P.
Right arrow Articles by Buckley, A. R.

Endocrinology, Vol 133, 3062-3065, Copyright © 1993 by Endocrine Society

ARTICLES

Nuclear co-localization of prolactin and the prolactin receptor in rat Nb2 node lymphoma cells

YP Rao, MD Olson, DJ Buckley and AR Buckley
Department of Pharmacology and Toxicology, University of North Dakota School of Medicine, Grand Forks 58202.

Previous studies have indicated that prolactin (PRL) interacts with specific, high affinity, immunoreactive binding sites within isolated rat hepatocyte nuclei. Moreover, endogenous PRL appears to be bound to this site. However, it remained important to demonstrate nuclear PRL receptors and hormonal translocation in an intact cell system. Therefore, we sought nuclear translocation of PRL and its receptor in the nucleus of PRL-dependent Nb2 node lymphoma cells. Utilizing immunofluorescence (IF) microscopy, growth-arrested cells were found to constitutively express the PRL receptor in the nucleus and in the membrane/cytosol compartments. Addition of PRL stimulated rapid hormone internalization followed by translocation to the nucleus within 6-12 hrs. The translocation of PRL was found to be reversible and dependent upon ATP. These results indicate that an early event coupled to the mitogenic action of PRL in Nb2 cells is hormone transport to the nucleus during the G1 and S phases of cell cycle. Once in the nucleus, PRL bound to its receptor may directly influence gene transcription.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J.-C. Lu, T. M. Piazza, and L. A. Schuler
Proteasomes Mediate Prolactin-induced Receptor Down-regulation and Fragment Generation in Breast Cancer Cells
J. Biol. Chem., October 7, 2005; 280(40): 33909 - 33916.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Ito, M. Nakasato, T. Suzuki, S. Sakai, M. Nagata, and F. Aoki
Localization of Janus Kinase 2 to the Nuclei of Mature Oocytes and Early Cleavage Stage Mouse Embryos
Biol Reprod, July 1, 2004; 71(1): 89 - 96.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. C. Mertani, M. Raccurt, A. Abbate, J. Kindblom, J. Tornell, N. Billestrup, Y. Usson, G. Morel, and P. E. Lobie
Nuclear Translocation and Retention of Growth Hormone
Endocrinology, July 1, 2003; 144(7): 3182 - 3195.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. E. Freeman, B. Kanyicska, A. Lerant, and G. Nagy
Prolactin: Structure, Function, and Regulation of Secretion
Physiol Rev, October 1, 2000; 80(4): 1523 - 1631.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M Perrot-Applanat, O Gualillo, H Buteau, M Edery, and P. Kelly
Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation
J. Cell Sci., January 5, 1997; 110(9): 1123 - 1132.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1993 by The Endocrine Society