help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park-Sarge, O. K.
Right arrow Articles by Mayo, K. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park-Sarge, O. K.
Right arrow Articles by Mayo, K. E.

Endocrinology, Vol 134, 709-718, Copyright © 1994 by Endocrine Society

ARTICLES

Regulation of the progesterone receptor gene by gonadotropins and cyclic adenosine 3',5'-monophosphate in rat granulosa cells

OK Park-Sarge and KE Mayo
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208.

We have examined the effects of the pituitary gonadotropins on expression of the progesterone receptor (PR) gene in rat ovary and in granulosa cell cultures. Both gonadotropins (LH/hCG and FSH) rapidly induce PR mRNA expression in the granulosa cells of preovulatory follicles in vivo. Gonadotropins also effectively induce PR mRNA expression in primary cultures of rat granulosa cells, and this action can be mimicked by agents that elevate intracellular cAMP (forskolin or 8-bromo-cAMP). Estrogen does not induce PR mRNA expression in these cells. The cAMP-induced PR mRNA expression in rat granulosa cells is blocked by an inhibitor of transcription, but not by an inhibitor of protein synthesis. Structural characterization of the rat PR gene 5'- flanking region indicates that the proximal promoter does not contain a consensus cAMP response element. However, when luciferase fusion genes containing a 1375-basepair rat PR gene promoter were transiently transfected into rat granulosa cells, luciferase activity could be stimulated several-fold by hCG or forskolin, but not by estrogen. These results indicate that gonadotropins, most likely acting through a pathway mediated by cAMP, are able to stimulate transcription of the PR gene in rat granulosa cells and suggest a mechanism for regulating the intraovarian actions of progesterone.


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
M. P. Flynn, E. T. Maizels, A. B. Karlsson, T. McAvoy, J.-H. Ahn, A. C. Nairn, and M. Hunzicker-Dunn
Luteinizing Hormone Receptor Activation in Ovarian Granulosa Cells Promotes Protein Kinase A-Dependent Dephosphorylation of Microtubule-Associated Protein 2D
Mol. Endocrinol., July 1, 2008; 22(7): 1695 - 1710.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Glaser, S. DeMorrow, H. Francis, Y. Ueno, E. Gaudio, S. Vaculin, J. Venter, A. Franchitto, P. Onori, B. Vaculin, et al.
Progesterone stimulates the proliferation of female and male cholangiocytes via autocrine/paracrine mechanisms
Am J Physiol Gastrointest Liver Physiol, July 1, 2008; 295(1): G124 - G136.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. J. Peluso, J. Romak, and X. Liu
Progesterone Receptor Membrane Component-1 (PGRMC1) Is the Mediator of Progesterone's Antiapoptotic Action in Spontaneously Immortalized Granulosa Cells As Revealed by PGRMC1 Small Interfering Ribonucleic Acid Treatment and Functional Analysis of PGRMC1 Mutations
Endocrinology, February 1, 2008; 149(2): 534 - 543.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. L. Kipp, S. M. Kilen, T. K. Woodruff, and K. E. Mayo
Activin Regulates Estrogen Receptor Gene Expression in the Mouse Ovary
J. Biol. Chem., December 14, 2007; 282(50): 36755 - 36765.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Lindeberg, K. Carlstrom, O. Ritvos, and O. Hovatta
Gonadotrophin stimulation of non-luteinized granulosa cells increases steroid production and the expression of enzymes involved in estrogen and progesterone synthesis
Hum. Reprod., February 1, 2007; 22(2): 401 - 406.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
L. Engmann, R. Losel, M. Wehling, and J. J. Peluso
Progesterone Regulation of Human Granulosa/Luteal Cell Viability by an RU486-Independent Mechanism
J. Clin. Endocrinol. Metab., December 1, 2006; 91(12): 4962 - 4968.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
G. S. Palanisamy, Y.-P. Cheon, J. Kim, A. Kannan, Q. Li, M. Sato, S. R. Mantena, R. L. Sitruk-Ware, M. K. Bagchi, and I. C. Bagchi
A Novel Pathway Involving Progesterone Receptor, Endothelin-2, and Endothelin Receptor B Controls Ovulation in Mice
Mol. Endocrinol., November 1, 2006; 20(11): 2784 - 2795.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. D. Burkart, A. Mukherjee, and K. E. Mayo
Mechanism of Repression of the Inhibin {alpha}-Subunit Gene by Inducible 3',5'-Cyclic Adenosine Monophosphate Early Repressor
Mol. Endocrinol., March 1, 2006; 20(3): 584 - 597.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. M. A. Tullet, V. Pocock, J. H. Steel, R. White, S. Milligan, and M. G. Parker
Multiple Signaling Defects in the Absence of RIP140 Impair Both Cumulus Expansion and Follicle Rupture
Endocrinology, September 1, 2005; 146(9): 4127 - 4137.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. F. Couse, M. M. Yates, B. J. Deroo, and K. S. Korach
Estrogen Receptor-{beta} Is Critical to Granulosa Cell Differentiation and the Ovulatory Response to Gonadotropins
Endocrinology, August 1, 2005; 146(8): 3247 - 3262.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. Hasegawa, A. Yanaihara, S. Iwasaki, Y. Otsuka, M. Negishi, T. Akahane, and T. Okai
Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality
Hum. Reprod., August 1, 2005; 20(8): 2194 - 2200.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
E. M. Foecking, M. Szabo, N. B. Schwartz, and J. E. Levine
Neuroendocrine Consequences of Prenatal Androgen Exposure in the Female Rat: Absence of Luteinizing Hormone Surges, Suppression of Progesterone Receptor Gene Expression, and Acceleration of the Gonadotropin-Releasing Hormone Pulse Generator
Biol Reprod, June 1, 2005; 72(6): 1475 - 1483.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. D. Burkart, A. Mukherjee, E. Sterneck, P. F. Johnson, and K. E. Mayo
Repression of the Inhibin {alpha}-Subunit Gene by the Transcription Factor CCAAT/Enhancer-Binding Protein-{beta}
Endocrinology, April 1, 2005; 146(4): 1909 - 1921.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. M. Quirk, R. G. Cowan, and R. M. Harman
Progesterone Receptor and the Cell Cycle Modulate Apoptosis in Granulosa Cells
Endocrinology, November 1, 2004; 145(11): 5033 - 5043.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Shimada, M. Nishibori, Y. Yamashita, J. Ito, T. Mori, and J. S. Richards
Down-Regulated Expression of A Disintegrin and Metalloproteinase with Thrombospondin-Like Repeats-1 by Progesterone Receptor Antagonist Is Associated with Impaired Expansion of Porcine Cumulus-Oocyte Complexes
Endocrinology, October 1, 2004; 145(10): 4603 - 4614.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
B. Mulac-Jericevic and O. M Conneely
Reproductive tissue selective actions of progesterone receptors
Reproduction, August 1, 2004; 128(2): 139 - 146.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Gava, C. L. Clarke, K. Byth, R. L. Arnett-Mansfield, and A. deFazio
Expression of Progesterone Receptors A and B in the Mouse Ovary during the Estrous Cycle
Endocrinology, July 1, 2004; 145(7): 3487 - 3494.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D.-S. Son, K. F. Roby, and P. F. Terranova
Tumor Necrosis Factor-{alpha} Induces Serum Amyloid A3 in Mouse Granulosa Cells
Endocrinology, May 1, 2004; 145(5): 2245 - 2252.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J.-Y. Park, F. Richard, S.-Y. Chun, J.-H. Park, E. Law, K. Horner, S-L C. Jin, and M. Conti
Phosphodiesterase Regulation Is Critical for the Differentiation and Pattern of Gene Expression in Granulosa Cells of the Ovarian Follicle
Mol. Endocrinol., June 1, 2003; 17(6): 1117 - 1130.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. Shao, E. Markstrom, P. A. Friberg, M. Johansson, and H. Billig
Expression of Progesterone Receptor (PR) A and B Isoforms in Mouse Granulosa Cells: Stage-Dependent PR-Mediated Regulation of Apoptosis and Cell Proliferation
Biol Reprod, March 1, 2003; 68(3): 914 - 921.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. Hrabovszky and S. L. Petersen
Increased Concentrations of Radioisotopically-labeled Complementary Ribonucleic Acid Probe, Dextran Sulfate, and Dithiothreitol in the Hybridization Buffer Can Improve Results of In Situ Hybridization Histochemistry
J. Histochem. Cytochem., October 1, 2002; 50(10): 1389 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
N. Danilovich, D. Javeshghani, W. Xing, and M. R. Sairam
Endocrine Alterations and Signaling Changes Associated with Declining Ovarian Function and Advanced Biological Aging in Follicle-Stimulating Hormone Receptor Haploinsufficient Mice
Biol Reprod, August 1, 2002; 67(2): 370 - 378.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J.J. Peluso, G. Fernandez, A. Pappalardo, and B.A. White
Membrane-Initiated Events Account for Progesterone's Ability to Regulate Intracellular Free Calcium Levels and Inhibit Rat Granulosa Cell Mitosis
Biol Reprod, August 1, 2002; 67(2): 379 - 385.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
M. Shimada and T. Terada
FSH and LH induce progesterone production and progesterone receptor synthesis in cumulus cells: a requirement for meiotic resumption in porcine oocytes
Mol. Hum. Reprod., July 1, 2002; 8(7): 612 - 618.
[Abstract] [Full Text] [PDF]

Home page
 Recent Prog Horm ResHome page
O. M. Conneely, B. Mulac-Jericevic, F. DeMayo, J. P. Lydon, and B. W. O'Malley
Reproductive Functions of Progesterone Receptors
Recent Prog. Horm. Res., January 1, 2002; 57(1): 339 - 355.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. M. Salvador, Y. Park, J. Cottom, E. T. Maizels, J. C. R. Jones, R. V. Schillace, D. W. Carr, P. Cheung, C. D. Allis, J. L. Jameson, et al.
Follicle-stimulating Hormone Stimulates Protein Kinase A-mediated Histone H3 Phosphorylation and Acetylation Leading to Select Gene Activation in Ovarian Granulosa Cells
J. Biol. Chem., October 19, 2001; 276(43): 40146 - 40155.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Sakamoto, K. Ukena, and K. Tsutsui
Effects of Progesterone Synthesized De Novo in the Developing Purkinje Cell on Its Dendritic Growth and Synaptogenesis
J. Neurosci., August 15, 2001; 21(16): 6221 - 6232.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J.J. Peluso, G. Fernandez, A. Pappalardo, and B.A. White
Characterization of a Putative Membrane Receptor for Progesterone in Rat Granulosa Cells
Biol Reprod, July 1, 2001; 65(1): 94 - 101.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Guo, L. Savage, K. D. Sarge, and O.-K. Park-Sarge
Gonadotropins Decrease Estrogen Receptor-{beta} Messenger Ribonucleic Acid Stability in Rat Granulosa Cells
Endocrinology, June 1, 2001; 142(6): 2230 - 2237.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
E. T. Maizels, A. Mukherjee, G. Sithanandam, C. A. Peters, J. Cottom, K. E. Mayo, and M. Hunzicker-Dunn
Developmental Regulation of Mitogen-Activated Protein Kinase-Activated Kinases-2 and -3 (MAPKAPK-2/-3) in Vivo during Corpus Luteum Formation in the Rat
Mol. Endocrinol., May 1, 2001; 15(5): 716 - 733.
[Abstract] [Full Text]

Home page
 Mol Hum ReprodHome page
J.-I. Park, W.-J. Kim, L. Wang, H.-J. Park, J. Lee, J.-H. Park, H.-B. Kwon, A. Tsafriri, and S.-Y. Chun
Involvement of progesterone in gonadotrophin-induced pituitary adenylate cyclase-activating polypeptide gene expression in pre-ovulatory follicles of rat ovary
Mol. Hum. Reprod., March 1, 2000; 6(3): 238 - 245.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. El-Hefnawy, P. R. Manna, M. Luconi, E. Baldi, J. P. Slotte, and I. Huhtaniemi
Progesterone Action in a Murine Leydig Tumor Cell Line (mLTC-1), Possibly through a Nonclassical Receptor Type
Endocrinology, January 1, 2000; 141(1): 247 - 255.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Szabo, S. M. Kilen, S. J. Nho, and N. B. Schwartz
Progesterone Receptor A and B Messenger Ribonucleic Acid Levels in the Anterior Pituitary of Rats Are Regulated by Estrogen
Biol Reprod, January 1, 2000; 62(1): 95 - 102.
[Abstract] [Full Text]

Home page
 Reproductive SciencesHome page
O. M. Conneely, J. P. Lydon, F. De Mayo, and B. W. O'Malley
Reproductive Functions of the Progesterone Receptor
Reproductive Sciences, January 1, 2000; 7(1_suppl): S25 - S32.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
J. F. Couse, D. O. Bunch, J. Lindzey, D. W. Schomberg, and K. S. Korach
Prevention of the Polycystic Ovarian Phenotype and Characterization of Ovulatory Capacity in the Estrogen Receptor-{alpha} Knockout Mouse
Endocrinology, December 1, 1999; 140(12): 5855 - 5865.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
C. Ko, Y.-H. In, and O.-K. Park-Sarge
Role of Progesterone Receptor Activation in Pituitary Adenylate Cyclase Activating Polypeptide Gene Expression in Rat Ovary
Endocrinology, November 1, 1999; 140(11): 5185 - 5194.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
M. L. O’Brien, K. Park, Y. In, and O.-K. Park-Sarge
Characterization of Estrogen Receptor-{beta} (ER{beta}) Messenger Ribonucleic Acid and Protein Expression in Rat Granulosa Cells
Endocrinology, October 1, 1999; 140(10): 4530 - 4541.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
C. L. Chaffin, R. L. Stouffer, and D. M. Duffy
Gonadotropin and Steroid Regulation of Steroid Receptor and Aryl Hydrocarbon Receptor Messenger Ribonucleic Acid in Macaque Granulosa Cells during the Periovulatory Interval
Endocrinology, October 1, 1999; 140(10): 4753 - 4760.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
D. W. Schomberg, J. F. Couse, A. Mukherjee, D. B. Lubahn, M. Sar, K. E. Mayo, and K. S. Korach
Targeted Disruption of the Estrogen Receptor-{alpha} Gene in Female Mice: Characterization of Ovarian Responses and Phenotype in the Adult
Endocrinology, June 1, 1999; 140(6): 2733 - 2744.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
J.J. Peluso and A. Pappalardo
Progesterone Maintains Large Rat Granulosa Cell Viability Indirectly by Stimulating Small Granulosa Cells to Synthesize Basic Fibroblast Growth Factor
Biol Reprod, February 1, 1999; 60(2): 290 - 296.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
D. M. Duffy, D. R. Stewart, and R. L. Stouffer
Titrating Luteinizing Hormone Replacement to Sustain the Structure and Function of the Corpus Luteum after Gonadotropin-Releasing Hormone Antagonist Treatment in Rhesus Monkeys
J. Clin. Endocrinol. Metab., January 1, 1999; 84(1): 342 - 349.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
A. Mukherjee, J. Urban, P. Sassone-Corsi, and K. E. Mayo
Gonadotropins Regulate Inducible Cyclic Adenosine 3',5'-Monophosphate Early Repressor in the Rat Ovary: Implications for Inhibin {alpha} Subunit Gene Expression
Mol. Endocrinol., June 1, 1998; 12(6): 785 - 800.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
M. Byers, G. G. J. M. Kuiper, J.-A. Gustafsson, and O.-K. Park-Sarge
Estrogen Receptor-{beta} mRNA Expression in Rat Ovary: Down-Regulation by Gonadotropins
Mol. Endocrinol., February 1, 1997; 11(2): 172 - 182.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
J P Lydon, F J DeMayo, C R Funk, S K Mani, A R Hughes, C A Montgomery, G Shyamala, O M Conneely, and B W O'Malley
Mice lacking progesterone receptor exhibit pleiotropic reproductive abnormalities.
Genes & Dev., September 15, 1995; 9(18): 2266 - 2278.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1994 by The Endocrine Society