Endocrinology, Vol 135, 387-394, Copyright © 1994 by Endocrine Society

ARTICLES

Cloning of a complementary deoxyribonucleic acid encoding the murine homolog of the very low density lipoprotein/apolipoprotein-E receptor: expression pattern and assignment of the gene to mouse chromosome 19 [published erratum appears in Endocrinology 1995 Feb;136(2):795]

ME Gafvels, LG Paavola, CO Boyd, PM Nolan, F Wittmaack, A Chawla, MA Lazar, M Bucan, BO Angelin and JF Strauss 3rd
Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia 19104.

We report the cloning of a complementary DNA for the mouse homolog of the very low density lipoprotein (VLDL)/apolipoprotein-E receptor (VLDLR), the deduced amino acid sequence of the protein, and the mapping of the gene encoding the receptor to mouse chromosome 19. Northern hybridization revealed that the VLDLR messenger RNA (mRNA) is most abundant in skeletal muscle, heart, kidney, and brain. It was also detected in lung and in low levels in liver, but it was not found in spleen or testes. Levels of VLDLR mRNA in mouse placenta increased from days 8-18 of gestation. The VLDLR mRNA was induced in 3T3-L1 cells undergoing differentiation into adipocytes. The increase in VLDLR mRNA paralleled the rise in lipoprotein lipase and hormone-sensitive lipase mRNAs. However, VLDLR and low density lipoprotein receptor-related protein were increased in the presence of retinoic acid, whereas the induction of lipoprotein lipase and hormone-sensitive lipase mRNAs was inhibited. Our observations demonstrate regulated expression of the VLDLR gene in placenta and adipocytes, where the receptor protein may play roles in the uptake of triglyceride-rich particles for storage of lipid (adipocytes) or for lipid transport to the fetus (placenta). The availability of a murine complementary DNA probe and the knowledge of the map position of the VLDLR gene in the mouse genome will facilitate studies on the function and regulation of this protein.

This article has been cited by other articles:

				J. A. McConihay, P. S. Horn, and L. A. Woollett Effect of maternal hypercholesterolemia on fetal sterol metabolism in the Golden Syrian hamster J. Lipid Res., July 1, 2001; 42(7): 1111 - 1119. [Abstract] [Full Text] [PDF]

				L. Umans, L. Overbergh, L. Serneels, I. Tesseur, and F. Van Leuven Analysis of Expression of Genes Involved in Apolipoprotein E-Based Lipoprotein Metabolism in Pregnant Mice Deficient in the Receptor-Associated Protein, the Low Density Lipoprotein Receptor, or Apolipoprotein E Biol Reprod, November 1, 1999; 61(5): 1216 - 1225. [Abstract] [Full Text]

				R. Savonen, L. M. Obermoeller, J. S. Trausch-Azar, A. L. Schwartz, and G. Bu The Carboxyl-terminal Domain of Receptor-associated Protein Facilitates Proper Folding and Trafficking of the Very Low Density Lipoprotein Receptor by Interaction with the Three Amino-terminal Ligand-binding Repeats of the Receptor J. Biol. Chem., September 3, 1999; 274(36): 25877 - 25882. [Abstract] [Full Text] [PDF]

				R. Kreuter, A. K. Soutar, and D. P. Wade Transcription factors CCAAT/enhancer-binding protein ß and nuclear factor-Y bind to discrete regulatory elements in the very low density lipoprotein receptor promoter J. Lipid Res., March 1, 1999; 40(3): 376 - 386. [Abstract] [Full Text]

				J. Magrané, M. Reina, R. Pagan, A. Luna, R. P. Casaroli-Marano, B. Angelin, M. Gåfvels, and S. Vilaró Bovine aortic endothelial cells express a variant of the very low density lipoprotein receptor that lacks the O-linked sugar domain J. Lipid Res., November 1, 1998; 39(11): 2172 - 2181. [Abstract] [Full Text]

				A. K. Hatzopoulos, A. Rigotti, R. D. Rosenberg, and M. Krieger Temporal and spatial pattern of expression of the HDL receptor SR-BI during murine embryogenesis J. Lipid Res., March 1, 1998; 39(3): 495 - 508. [Abstract] [Full Text]

				K. L. Wyne and L. A. Woollett Transport of maternal LDL and HDL to the fetal membranes and placenta of the Golden Syrian hamster is mediated by receptor-dependent and receptor-independent processes J. Lipid Res., March 1, 1998; 39(3): 518 - 530. [Abstract] [Full Text]

				H. Bujo, M. Hermann, K. A. Lindstedt, J. Nimpf, and W. J. Schneider Low Density Lipoprotein Receptor Gene Family Members Mediate Yolk Deposition J. Nutr., May 1, 1997; 127 (5): 801S - 801S. [Abstract] [Full Text] [PDF]

				S. Novak, T. Hiesberger, W. J. Schneider, and J. Nimpf A New Low Density Lipoprotein Receptor Homologue with 8 Ligand Binding Repeats in Brain of Chicken and Mouse J. Biol. Chem., May 17, 1996; 271(20): 11732 - 11736. [Abstract] [Full Text] [PDF]

				D.-H. Kim, H. Iijima, K. Goto, J. Sakai, H. Ishii, H.-J. Kim, H. Suzuki, H. Kondo, S. Saeki, and T. Yamamoto Human Apolipoprotein E Receptor 2 J. Biol. Chem., April 5, 1996; 271(14): 8373 - 8380. [Abstract] [Full Text] [PDF]

				K. Kobayashi, K. Oka, T. Forte, B. Ishida, B. Teng, K. Ishimura-Oka, M. Nakamuta, and L. Chan Reversal of Hypercholesterolemia in Low Density Lipoprotein Receptor Knockout Mice by Adenovirus-mediated Gene Transfer of the Very Low Density Lipoprotein Receptor J. Biol. Chem., March 22, 1996; 271(12): 6852 - 6860. [Abstract] [Full Text] [PDF]

				H. Bujo, K. A. Lindstedt, M. Hermann, L. M. Dalmau, J. Nimpf, and W. J. Schneider Chicken Oocytes and Somatic Cells Express Different Splice Variants of a Multifunctional Receptor J. Biol. Chem., October 6, 1995; 270(40): 23546 - 23551. [Abstract] [Full Text] [PDF]

				L. Umans, L. Serneels, L. Overbergh, K. Lorent, F. Van Leuven, and H. Van den Berghe Targeted Inactivation of the Mouse alpha(2)-Macroglobulin Gene J. Biol. Chem., August 25, 1995; 270(34): 19778 - 19785. [Abstract] [Full Text] [PDF]

				T. Hiesberger, M. Hermann, L. Jacobsen, S. Novak, R. A. Hodits, H. Bujo, M. Meilinger, M. Httinger, W. J. Schneider, and J. Nimpf The Chicken Oocyte Receptor for Yolk Precursors as a Model for Studying the Action of Receptor-associated Protein and Lactoferrin J. Biol. Chem., August 4, 1995; 270(31): 18219 - 18226. [Abstract] [Full Text] [PDF]

				A. A. Troussard, J. Khallou, C. J. Mann, P. André, D. K. Strickland, B. E. Bihain, and F. T. Yen Inhibitory Effect on the Lipolysis-stimulated Receptor of the 39-kDa Receptor-associated Protein J. Biol. Chem., July 21, 1995; 270(29): 17068 - 17071. [Abstract] [Full Text] [PDF]

				L. Jacobsen, M. Hermann, Päi. M. Vieira, W. J. Schneider, and J. Nimpf The Chicken Oocyte Receptor for Lipoprotein Deposition Recognizes alpha(2)-Macroglobulin J. Biol. Chem., March 24, 1995; 270(12): 6468 - 6475. [Abstract] [Full Text] [PDF]

				R. Sakthivel, J.-C. Zhang, D. K. Strickland, M. Gafvels, and K. R. McCrae Regulation of the Ligand Binding Activity of the Human Very Low Density Lipoprotein Receptor by Protein Kinase C-dependent Phosphorylation J. Biol. Chem., January 5, 2001; 276(1): 555 - 562. [Abstract] [Full Text] [PDF]

				D. K. Swertfeger, G. Bu, and D. Y. Hui Low Density Lipoprotein Receptor-related Protein Mediates Apolipoprotein E Inhibition of Smooth Muscle Cell Migration J. Biol. Chem., February 1, 2002; 277(6): 4141 - 4146. [Abstract] [Full Text] [PDF]