help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kaji, H.
Right arrow Articles by Chihara, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaji, H.
Right arrow Articles by Chihara, K.

Endocrinology, Vol 135, 484-487, Copyright © 1994 by Endocrine Society

ARTICLES

Calcitonin inhibits osteopontin mRNA expression in isolated rabbit osteoclasts

H Kaji, T Sugimoto, A Miyauchi, M Fukase, K Tezuka, Y Hakeda, M Kumegawa and K Chihara
Department of Medicine, Kobe University School of Medicine, Japan.

Recent evidence indicates that osteopontin (Opn), one of the bone matrix proteins, plays an important role in the attachment of osteoclasts to bone matrix. Besides being elaborated by osteoblasts, this protein is also produced by osteoclasts. The present study was performed to examine the effect of calcitonin (CT) on Opn mRNA expression of isolated rabbit osteoclasts and to clarify the second messenger signaling of this effect. Eel CT inhibited Opn mRNA expression as well as bone-resorbing activity of isolated rabbit osteoclasts. Eel CT caused a transient increase in intracellular calcium followed by a sustained increase as well as an increase in cAMP production in these cells. Dibutyryl-cAMP (10(-4) M) and Sp-cAMPS (10(- 4) M), an activator of cAMP-dependent protein kinase (PKA), as well as A23187 (10(-7) M), a calcium ionophore, and phorbol myristate acetate (10(-7) M), an activator of protein kinase C (PKC), caused a significant inhibition of Opn mRNA expression, and suppressed bone- resorbing activity of isolated osteoclasts. The present study is the first to demonstrate that CT inhibits Opn mRNA expression in isolated rabbit osteoclasts, presumably through the activation of PKA and calcium/PKC pathways, by which the bone-resorbing activity might be attenuated subsequently.


This article has been cited by other articles:


Home page
 EndocrinologyHome page
K. Fuller, B. Kirstein, and T. J. Chambers
Murine Osteoclast Formation and Function: Differential Regulation by Humoral Agents
Endocrinology, April 1, 2006; 147(4): 1979 - 1985.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. Boissy, F. Saltel, C. Bouniol, P. Jurdic, and I. Machuca-Gayet
Transcriptional Activity of Nuclei in Multinucleated Osteoclasts and Its Modulation by Calcitonin
Endocrinology, May 1, 2002; 143(5): 1913 - 1921.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y. Asou, S. R. Rittling, H. Yoshitake, K. Tsuji, K. Shinomiya, A. Nifuji, D. T. Denhardt, and M. Noda
Osteopontin Facilitates Angiogenesis, Accumulation of Osteoclasts, and Resorption in Ectopic Bone
Endocrinology, March 1, 2001; 142(3): 1325 - 1332.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
J. Sodek, B. Ganss, and M.D. McKee
Osteopontin
Critical Reviews in Oral Biology & Medicine, January 1, 2000; 11(3): 279 - 303.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T Sato, M del Carmen Ovejero, P Hou, A. Heegaard, M Kumegawa, N. Foged, and J. Delaisse
Identification of the membrane-type matrix metalloproteinase MT1-MMP in osteoclasts
J. Cell Sci., January 3, 1997; 110(5): 589 - 596.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1994 by The Endocrine Society