Endocrinology, Vol 135, 1073-1076, Copyright © 1994 by Endocrine Society

ARTICLES

Growth hormone (GH)-releasing hormone secretion is stimulated by a new GH-releasing hexapeptide in sheep

V Guillaume, E Magnan, M Cataldi, A Dutour, N Sauze, M Renard, H Razafindraibe, B Conte-Devolx, R Deghenghi and V Lenaerts
Laboratoire de Neuroendocrinologie Experimentale, Institut Federatif Jean Roche, INSERM U-297, Faculte de Medecine Nord, Marseille, France.

The acute effect of a new GH-releasing peptide, hexarelin (1 mg, iv), on GH secretion and the mechanisms involved in its changes were investigated in conscious sheep. Peripheral GH levels and GH-releasing hormone (GHRH) and somatostatin concentrations in hypophysial portal blood were measured in six rams. An increase in jugular GH levels was observed 15 min after hexarelin injection (9.1 +/- 1.8 vs. 3.9 +/- 0.8 ng/ml; P < 0.05). This was associated with a stimulation of GHRH release into hypophysial portal blood (145.4 +/- 19.9 vs. 59.2 +/- 10.8 pg/ml; P < 0.01) without a change in somatostatin secretion. Our data indicate that GH-releasing peptide-induced GH stimulation in the sheep involves an activation of GHRH neurons in addition to the previously demonstrated direct effect on the pituitary cells.

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