help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ding, Y. Q.
Right arrow Articles by Bagchi, I. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ding, Y. Q.
Right arrow Articles by Bagchi, I. C.

Endocrinology, Vol 135, 2265-2274, Copyright © 1994 by Endocrine Society

ARTICLES

Progesterone stimulates calcitonin gene expression in the uterus during implantation

YQ Ding, LJ Zhu, MK Bagchi and IC Bagchi
Population Council, New York, New York 10021.

Implantation of the mammalian embryo into the wall of the uterus is regulated by a timely interplay of the ovarian hormones, estrogen and progesterone. These hormones orchestrate a set of modifications in the uterine endometrium that transforms it from a nonreceptive to a receptive phase allowing the implantation of the developing blastocyst. The molecular and cellular mechanisms underlying this complex process, however, remain largely unknown. To investigate the endocrine basis of uterine receptivity, we employed a gene expression screen technique to identify factors whose expressions are modulated in the rat uterus in response to estrogen and progesterone at the onset of implantation. Here we report that the expression of calcitonin, a peptide hormone involved in calcium homeostasis, is markedly enhanced in the uterus during pregnancy. By Northern blot analysis, we show that the synthesis of calcitonin messenger RNA is induced at the time of implantation. Immunocytochemistry with calcitonin antibody demonstrates further that the peptide is localized in the glandular epithelial cells of the uterus. The antiprogestin drug RU486, which is known to block implantation, abolishes calcitonin expression, suggesting a regulatory role for progesterone in this process. Consistent with this observation, progesterone significantly stimulates calcitonin messenger RNA and protein synthesis in the uteri of ovariectomized animals. Our study, therefore, identifies calcitonin as a stage- and cell-specific marker of progesterone action in the uterus during pregnancy. Estrogen exhibits no significant effect on calcitonin expression when administered alone to ovariectomized animals. However, a low dose of estrogen synergizes with progesterone, and a high dose antagonizes progesterone-mediated gene induction. Both estrogen and progesterone, therefore, modulate calcitonin gene expression in the uterus. The stage- specific regulation of calcitonin is apparently determined by the relative concentrations and the sequences of appearance of these two hormones and possibly other as yet unknown regulatory factors during pregnancy. We propose that calcitonin, a known regulator of calcium levels in the bone and kidney, may play an important regulatory role in the uterus of pregnant animals during the early events leading to implantation of the embryo.


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
S. M Krzysik-Walker, O. M Ocon-Grove, S. B Maddineni, G. L Hendricks III, and R. Ramachandran
Identification of Calcitonin Expression in the Chicken Ovary: Influence of Follicular Maturation and Ovarian Steroids
Biol Reprod, October 1, 2007; 77(4): 626 - 635.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
H. Achache and A. Revel
Endometrial receptivity markers, the journey to successful embryo implantation
Hum. Reprod. Update, November 1, 2006; 12(6): 731 - 746.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Q. Li, M. K. Bagchi, and I. C. Bagchi
Identification of a Signaling Pathway Involving Progesterone Receptor, Calcitonin, and Tissue Tranglutaminase in Ishikawa Endometrial Cells
Endocrinology, May 1, 2006; 147(5): 2147 - 2154.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
P. Lalitkumar, J Sengupta, and D Ghosh
Endometrial tumor necrosis factor {alpha} (TNF{alpha}) is a likely mediator of early luteal phase mifepristone-mediated negative effector action on the preimplantation embryo
Reproduction, March 1, 2005; 129(3): 323 - 335.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
S Sukjumlong, A-M Dalin, L Sahlin, and E Persson
Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy
Reproduction, March 1, 2005; 129(3): 349 - 359.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
K. Y Lee and F. J DeMayo
Animal models of implantation
Reproduction, December 1, 2004; 128(6): 679 - 695.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. Chen, X. Xu, Y.-P. Cheon, M. K. Bagchi, and I. C. Bagchi
Estrogen Induces Expression of Secretory Leukocyte Protease Inhibitor in Rat Uterus
Biol Reprod, August 1, 2004; 71(2): 508 - 514.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. C. M. Simmen, R. R. Eason, J. R. McQuown, A. L. Linz, T.-J. Kang, L. Chatman Jr., S. R. Till, Y. Fujii-Kuriyama, F. A. Simmen, and S. P. Oh
Subfertility, Uterine Hypoplasia, and Partial Progesterone Resistance in Mice Lacking the Kruppel-like Factor 9/Basic Transcription Element-binding Protein-1 (Bteb1) Gene
J. Biol. Chem., July 9, 2004; 279(28): 29286 - 29294.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. Kumar, A. Brudney, Y.-P. Cheon, A. T. Fazleabas, and I. C. Bagchi
Progesterone Induces Calcitonin Expression in the Baboon Endometrium Within the Window of Uterine Receptivity
Biol Reprod, April 1, 2003; 68(4): 1318 - 1323.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
Y.-P. Cheon, Q. Li, X. Xu, F. J. DeMayo, I. C. Bagchi, and M. K. Bagchi
A Genomic Approach to Identify Novel Progesterone Receptor Regulated Pathways in the Uterus during Implantation
Mol. Endocrinol., December 1, 2002; 16(12): 2853 - 2871.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Li, J. Wang, D. R. Armant, M. K. Bagchi, and I. C. Bagchi
Calcitonin Down-regulates E-cadherin Expression in Rodent Uterine Epithelium during Implantation
J. Biol. Chem., November 22, 2002; 277(48): 46447 - 46455.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y.-P. Sun, T. J. Lee, and G. V. Shah
Calcitonin Expression in Rat Anterior Pituitary Gland Is Regulated by Ovarian Steroid Hormones
Endocrinology, October 1, 2002; 143(10): 4056 - 4064.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. A. Tverberg, M. F. Gustafson, T. L. Scott, I. V. Arzumanova, E. R. Provost, A. W. Yan, and S. A. Rawie
Induction of Calcitonin and Calcitonin Receptor Expression in Rat Mammary Tissue during Pregnancy
Endocrinology, October 1, 2000; 141(10): 3696 - 3702.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D. Ghosh, P.G.L. Lalitkumar, V. J. Wong, A.G. Hendrickx, and J. Sengupta
Preimplantation embryo morphology following early luteal phase anti-nidatory treatment with mifepristone (RU486) in the rhesus monkey
Hum. Reprod., January 1, 2000; 15(1): 180 - 188.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J Wang, L Mayernik, J. Schultz, and D. Armant
Acceleration of trophoblast differentiation by heparin-binding EGF-like growth factor is dependent on the stage-specific activation of calcium influx by ErbB receptors in developing mouse blastocysts
Development, January 1, 2000; 127(1): 33 - 44.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. W. Curtis, J. Clark, P. Myers, and K. S. Korach
Disruption of estrogen signaling does not prevent progesterone action in the estrogen receptor alpha  knockout mouse uterus
PNAS, March 30, 1999; 96(7): 3646 - 3651.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
W. Balkan, E. L. Oates, G. A. Howard, and B. A. Roos
Testes Exhibit Elevated Expression of Calcitonin Gene-Related Peptide Receptor Component Protein
Endocrinology, March 1, 1999; 140(3): 1459 - 1469.
[Abstract] [Full Text]

Home page
 Mol Hum ReprodHome page
K. Tatsumi, T. Higuchi, H. Fujiwara, T. Nakayama, K. Itoh, T. Mori, S. Fujii, and J. Fujita
Expression of calcium binding protein D-9k messenger RNA in the mouse uterine endometrium during implantation
Mol. Hum. Reprod., February 1, 1999; 5(2): 153 - 161.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
S. Kumar, L.-J. Zhu, M. Polihronis, S. T. Cameron, D. T. Baird, F. Schatz, A. Dua, Y.-K. Ying, M. K. Bagchi, and I. C. Bagchi
Progesterone Induces Calcitonin Gene Expression in Human Endometrium within the Putative Window of Implantation
J. Clin. Endocrinol. Metab., December 1, 1998; 83(12): 4443 - 4450.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
L.-J. Zhu, K. Cullinan-Bove, M. Polihronis, M. K. Bagchi, and I. C. Bagchi
Calcitonin Is a Progesterone-Regulated Marker That Forecasts the Receptive State of Endometrium during Implantation
Endocrinology, September 1, 1998; 139(9): 3923 - 3934.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J Wang, U. Rout, I. Bagchi, and D. Armant
Expression of calcitonin receptors in mouse preimplantation embryos and their function in the regulation of blastocyst differentiation by calcitonin
Development, January 11, 1998; 125(21): 4293 - 4302.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
L.-J. Zhu, M. K. Bagchi, and I. C. Bagchi
Attenuation of Calcitonin Gene Expression in Pregnant Rat Uterus Leads to a Block in Embryonic Implantation
Endocrinology, January 1, 1998; 139(1): 330 - 339.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. D. Graham and C. L. Clarke
Physiological Action of Progesterone in Target Tissues
Endocr. Rev., August 1, 1997; 18(4): 502 - 519.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1994 by The Endocrine Society