Endocrinology, Vol 136, 5236-5239, Copyright © 1995 by Endocrine Society

ARTICLES

A member of the CTF/NF-1 transcription factor family regulates murine growth hormone receptor gene promoter activity

L Zou and RK Menon
Department of Pediatrics, Children's Hosp. of Pittsburgh, University of Pittsburgh School of Medicine, PA 15213, USA.

Deletional analysis by transient transfection of COS-7 cells with the murine growth hormone (GH) receptor gene promoter for the L1 transcript identified a 16-bp enhancer element located approximately 3.0 kb upstream of the major transcription start sites. The sequence of this enhancer element suggested a binding motif for the CTF/NF-1 family of transcription factors. In electromobility shift assays (EMSA) this DNA- element formed a sequence-specific complex with nuclear proteins from COS-7 cells. Competition experiments with oligonucleotide containing a consensus binding motif for CTF/NF-1 protein(s) and supershift EMSA with an anti-CTF/NF-1 antibody established that a CTF/NF-1 like protein binds to this enhancer element.

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