| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Endocrinology, Vol 136, 2928-2936, Copyright © 1995 by Endocrine Society
ARTICLES |
C Breton, C Pechoux, G Morel and HH Zingg
Laboratory of Molecular Endocrinology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
The hypothalamic neuropeptide oxytocin (OT) stimulates the release of several pituitary hormones, including ACTH, LH, and PRL. Although specific OT receptors have been identified in anterior pituitary membranes, the structure and cellular localization of these binding sites have not been elucidated. We previously cloned a rat OT receptor (OTR) gene and showed that its expression in rat uterus results in several transcripts ranging in size from 2.9-6.7 kilobases. In this study we show, by using Northern blot analysis, reverse transcriptase- polymerase chain reaction, and ultrastructural in situ hybridization that the same OTR gene is also expressed in the pituitary, where it gives rise to a 6.7- and a 4.8-kilobase messenger RNA. Ultrastructural in situ hybridization combined with immunogold labeling indicated that pituitary OTR gene expression is highly cell-specific and restricted to lactotrophs. In accordance with this finding, only the lactotroph- derived cell line MMQ expressed the OTR gene among several pituitary cell lines tested. Northern blot analysis, reverse transcriptase- polymerase chain reaction, and in situ hybridization analysis indicated a dramatic increase in pituitary OTR gene expression at the end of gestation and after estrogen treatment. Our results suggest that the OT effect on lactotrophs is direct, whereas OT actions on gonadotrophs and corticotrophs are either indirect or mediated via different receptors. Moreover, our findings imply that OT exerts its full potential as a physiological PRL-releasing factor only towards the end of gestation, and that therefore the role of OT as a hypothalamic PRL-releasing factor may so far have been underestimated.
This article has been cited by other articles:
 |
|
 |
|
  J. E. Kennett, M. O. Poletini, C. A. Fitch, and M. E. Freeman Antagonism of Oxytocin Prevents Suckling- and Estradiol-Induced, But Not Progesterone-Induced, Secretion of Prolactin Endocrinology, May 1, 2009; 150(5): 2292 - 2299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Breton, M.-A. Lukaszewski, P.-Y. Risold, M. Enache, J. Guillemot, G. Riviere, F. Delahaye, J. Lesage, I. Dutriez-Casteloot, C. Laborie, et al. Maternal prenatal undernutrition alters the response of POMC neurons to energy status variation in adult male rat offspring Am J Physiol Endocrinol Metab, March 1, 2009; 296(3): E462 - E472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakamura, Y. Fujiwara, R. Mizutani, A. Sanbe, N. Miyauchi, M. Hiroyama, J. Yamauchi, T. Yamashita, S. Nakamura, T. Mori, et al. Effects of Vasopressin V1b Receptor Deficiency on Adrenocorticotropin Release from Anterior Pituitary Cells in Response to Oxytocin Stimulation Endocrinology, October 1, 2008; 149(10): 4883 - 4891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Delahaye, C. Breton, P.-Y. Risold, M. Enache, I. Dutriez-Casteloot, C. Laborie, J. Lesage, and D. Vieau Maternal Perinatal Undernutrition Drastically Reduces Postnatal Leptin Surge and Affects the Development of Arcuate Nucleus Proopiomelanocortin Neurons in Neonatal Male Rat Pups Endocrinology, February 1, 2008; 149(2): 470 - 475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. T. McKee, M. O. Poletini, R. Bertram, and M. E. Freeman Oxytocin Action at the Lactotroph Is Required for Prolactin Surges in Cervically Stimulated Ovariectomized Rats Endocrinology, October 1, 2007; 148(10): 4649 - 4657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mairesse, J. Lesage, C. Breton, B. Breant, T. Hahn, M. Darnaudery, S. L. Dickson, J. Seckl, B. Blondeau, D. Vieau, et al. Maternal stress alters endocrine function of the feto-placental unit in rats Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1526 - E1533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Molendi-Coste, L. Grumolato, C. Laborie, J. Lesage, E. Maubert, H. Ghzili, H. Vaudry, Y. Anouar, C. Breton, and D. Vieau Maternal Perinatal Undernutrition Alters Neuronal and Neuroendocrine Differentiation in the Rat Adrenal Medulla at Weaning Endocrinology, June 1, 2006; 147(6): 3050 - 3059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Levoye, B Mouillac, G Riviere, D Vieau, M Salzet, and C Breton Cloning, expression and pharmacological characterization of a vasopressin-related receptor in an annelid, the leech Theromyzon tessulatum J. Endocrinol., January 1, 2005; 184(1): 277 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-N. Spiess, N. Mueller, and R. Ivell Trehalose Is a Potent PCR Enhancer: Lowering of DNA Melting Temperature and Thermal Stabilization of Taq Polymerase by the Disaccharide Trehalose, Clin. Chem., July 1, 2004; 50(7): 1256 - 1259. [Full Text] [PDF]
|
|
|
|
|
|
M. Egli, R. Bertram, M. T. Sellix, and M. E. Freeman Rhythmic Secretion of Prolactin in Rats: Action of Oxytocin Coordinated by Vasoactive Intestinal Polypeptide of Suprachiasmatic Nucleus Origin Endocrinology, July 1, 2004; 145(7): 3386 - 3394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Champagne, P. Chretien, C. W. Stevenson, T. Y. Zhang, A. Gratton, and M. J. Meaney Variations in Nucleus Accumbens Dopamine Associated with Individual Differences in Maternal Behavior in the Rat J. Neurosci., April 28, 2004; 24(17): 4113 - 4123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Champagne, I. C. G. Weaver, J. Diorio, S. Sharma, and M. J. Meaney Natural Variations in Maternal Care Are Associated with Estrogen Receptor {alpha} Expression and Estrogen Sensitivity in the Medial Preoptic Area Endocrinology, November 1, 2003; 144(11): 4720 - 4724. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Pequeux, C. Breton, J.-C. Hendrick, M.-T. Hagelstein, H. Martens, R. Winkler, V. Geenen, and J.-J. Legros Oxytocin Synthesis and Oxytocin Receptor Expression by Cell Lines of Human Small Cell Carcinoma of the Lung Stimulate Tumor Growth through Autocrine/Paracrine Signaling Cancer Res., August 15, 2002; 62(16): 4623 - 4629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Tasiemski, H. Hammad, F. Vandenbulcke, C. Breton, T. J. Bilfinger, J. Pestel, and M. Salzet Presence of chromogranin-derived antimicrobial peptides in plasma during coronary artery bypass surgery and evidence of an immune origin of these peptides Blood, June 28, 2002; 100(2): 553 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gimpl and F. Fahrenholz The Oxytocin Receptor System: Structure, Function, and Regulation Physiol Rev, April 1, 2001; 81(2): 629 - 683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Schwartz Intercellular Communication in the Anterior Pituitary Endocr. Rev., October 1, 2000; 21(5): 488 - 513. [Abstract] [Full Text]
|
|
|
|
|
|
M. E. Freeman, B. Kanyicska, A. Lerant, and G. Nagy Prolactin: Structure, Function, and Regulation of Secretion Physiol Rev, October 1, 2000; 80(4): 1523 - 1631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Stefano, P. Cadet, C. Breton, Y. Goumon, V. Prevot, J. P. Dessaint, J.-C. Beauvillain, A. S. Roumier, I. Welters, and M. Salzet Estradiol-stimulated nitric oxide release in human granulocytes is dependent on intracellular calcium transients: evidence of a cell surface estrogen receptor Blood, June 15, 2000; 95(12): 3951 - 3958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B. Stefano, V. Prevot, J.-C. Beauvillain, P. Cadet, C. Fimiani, I. Welters, G. L. Fricchione, C. Breton, P. Lassalle, M. Salzet, et al. Cell-Surface Estrogen Receptors Mediate Calcium-Dependent Nitric Oxide Release in Human Endothelia Circulation, April 4, 2000; 101(13): 1594 - 1597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B. Stefano, V. Prevot, J.-C. Beauvillain, C. Fimiani, I. Welters, P. Cadet, C. Breton, J. Pestel, M. Salzet, and T. V. Bilfinger Estradiol Coupling to Human Monocyte Nitric Oxide Release Is Dependent on Intracellular Calcium Transients: Evidence for an Estrogen Surface Receptor J. Immunol., October 1, 1999; 163(7): 3758 - 3763. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Grazzini, C. Breton, S. Derick, M. Andres, D. Raufaste, F. Rickwaert, G. Boccara, P. Colson, N. C. Guérineau, C. Serradeil-Le Gal, et al. Vasopressin Receptors in Human Adrenal Medulla and Pheochromocytoma J. Clin. Endocrinol. Metab., June 1, 1999; 84(6): 2195 - 2203. [Abstract] [Full Text]
|
|
|
|
|
|
C. Rabadan-Diehl and G. Aguilera Glucocorticoids Increase Vasopressin V1b Receptor Coupling to Phospholipase C Endocrinology, July 1, 1998; 139(7): 3220 - 3226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gutkowska, M. Jankowski, C. Lambert, S. Mukaddam-Daher, H. H. Zingg, and S. M. McCann Oxytocin releases atrial natriuretic peptide by combining with oxytocin receptors in the heart PNAS, October 14, 1997; 94(21): 11704 - 11709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Breton and H. H. Zingg Expression and Region-Specific Regulation of the Oxytocin Receptor Gene in Rat Brain Endocrinology, May 1, 1997; 138(5): 1857 - 1862. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Evans, W. Forrest-Owen, and C. A. McArdle Oxytocin Receptor-Mediated Activation of Phosphoinositidase C and Elevation of Cytosolic Calcium in the Gonadotrope-Derived {alpha}T3-1 Cell Line Endocrinology, May 1, 1997; 138(5): 2049 - 2055. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
