Endocrinology, Vol 137, 42-46, Copyright © 1996 by Endocrine Society

ARTICLES

C-type natriuretic peptide as a possible local modulator of aldosterone secretion in bovine adrenal zona glomerulosa

M Kawai, M Naruse, T Yoshimoto, K Naruse, K Shionoya, M Tanaka, Y Morishita, Y Matsuda, R Demura and H Demura
Department of Medicine, Tokyo Women's Medical College, Japan.

Although atrial and brain natriuretic peptides are well known to be involved in the regulation of cardiovascular and endocrine functions as circulating hormones, the roles of the C-type natriuretic peptide (CNP) remain unknown. We examined the effects of CNP on the secretion of aldosterone and cyclic nucleotides from bovine adrenal zona glomerulosa cells in culture. CNP produced a dose-dependent increase in the basal secretion of cGMP, with an EC50 of 3.8 x 10(-10)M. CNP significantly inhibited the ACTH-induced increase in aldosterone and cAMP in a dose- related manner, with an IC50 of 3.6 x 10(-10)M. Although ACTH itself did not increase cGMP secretion, the addition of CNP elicited a significant increase in cGMP secretion. The effects of CNP on the basal secretion of cGMP and the ACTH-induced secretion of aldosterone were significantly reversed by a nonpeptide natriuretic peptide receptor antagonist, HS-142-1. CNP immunoreactivity was localized in the zona glomerulosa by immunohistochemical staining. In addition, expression of CNP messenger RNA and natriuretic peptide B receptor messenger RNA was demonstrated by RT-PCR in the zona glomerulosa tissue and cells in culture. These findings suggest that CNP is a local factor regulating ACTH-induced aldosterone secretion through a guanylyl cyclase-cGMP pathway.

This article has been cited by other articles:

				S. A. Andric, M. M. Janjic, N. J. Stojkov, and T. S. Kostic Protein kinase G-mediated stimulation of basal Leydig cell steroidogenesis Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1399 - E1408. [Abstract] [Full Text] [PDF]

				F. El-Gehani, M. Tena-Sempere, H. Ruskoaho, and I. Huhtaniemi Natriuretic Peptides Stimulate Steroidogenesis in the Fetal Rat Testis Biol Reprod, August 1, 2001; 65(2): 595 - 600. [Abstract] [Full Text] [PDF]

				A. Lacroix, N. N'Diaye, J. Tremblay, and P. Hamet Ectopic and Abnormal Hormone Receptors in Adrenal Cushing's Syndrome Endocr. Rev., February 1, 2001; 22(1): 75 - 110. [Abstract] [Full Text]

				S. J. Lee, S. Z. Kim, X. Cui, S. H. Kim, K. S. Lee, Y. J. Chung, and K. W. Cho C-type natriuretic peptide inhibits ANP secretion and atrial dynamics in perfused atria: NPR-B-cGMP signaling Am J Physiol Heart Circ Physiol, January 1, 2000; 278(1): H208 - H221. [Abstract] [Full Text] [PDF]

				M. Gunning, R. J. Solomon, F. H. Epstein, and P. Silva Role of guanylyl cyclase receptors for CNP in salt secretion by shark rectal gland Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1997; 273(4): R1400 - R1406. [Abstract] [Full Text] [PDF]