| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Endocrinology, Vol 137, 5269-5277, Copyright © 1996 by Endocrine Society
ARTICLES |
JP Witty, JT Bridgham and AL Johnson
Department of Biological Sciences, University of Notre Dame, Indiana 46556, USA.
Recent studies have demonstrated that ovarian follicle atresia occurs extensively before follicle selection into the avian preovulatory hierarchy, and that this process is mediated via granulosa cell apoptosis. Subsequent to follicle selection, granulosa cells are inherently resistant to apoptosis, and such resistance is correlated with increased expression of death suppressor genes such as bcl-xlong. In the present studies we used this avian ovary model system to 1) identify cellular characteristics and mechanisms related to apoptotic cell death of granulosa cells in vitro, and 2) further characterize functional differences between apoptosis-susceptible (4- to 8-mm follicle) and apoptosis-resistant (preovulatory follicle) granulosa cells. Treatment of granulosa cells from the largest preovulatory follicle with N-octanoylsphingosine (C8-ceramide) results in pronounced oligonucleosome formation, a hallmark of apoptosis. That this is indicative of programmed cell death is supported by an increased incidence of pyknotic nuclei and apoptotic bodies in C8-ceramide- treated samples compared to that in control cultured cells. Tumor necrosis factor-alpha, a stimulator of ceramide production, actively promotes oligonucleosome formation in apoptosis-susceptible, but not in apoptosis-resistant, granulosa cells. Induction of apoptosis is also observed after exposure of apoptosis-resistant granulosa cells to sphingomyelinase treatment and UV irradiation, which are known to stimulate endogenous ceramide production, and to the anticancer drug, daunorubicin, which initiates de novo ceramide biosynthesis via activation of ceramide synthase. Although treatment of granulosa cells with fumonisin B1, a specific ceramide synthase inhibitor, blocks daunorubicin-stimulated oligonucleosome formation, UV-induced cell death is unaffected. Taken together, these results demonstrate that pharmacological factors known to mimic the actions of ceramide or stimulate ceramide production can induce oligonucleosome formation and programmed cell death in granulosa cells. More importantly, however, the ability of a physiologically relevant initiator of ceramide biosynthesis, tumor necrosis factor-alpha, to promote cell death is evident only in apoptosis-susceptible granulosa cells collected from atresia-prone prehierarchal follicles. These data provide support for ceramide as an important intracellular signaling mechanism, mediating granulosa cell apoptosis and follicle atresia.
This article has been cited by other articles:
 |
|
 |
|
  L. J. Siskind, L. Feinstein, T. Yu, J. S. Davis, D. Jones, J. Choi, J. E. Zuckerman, W. Tan, R. B. Hill, J. M. Hardwick, et al. Anti-apoptotic Bcl-2 Family Proteins Disassemble Ceramide Channels J. Biol. Chem., March 14, 2008; 283(11): 6622 - 6630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A L Johnson, C. Ratajczak, M. J Haugen, H.-K. Liu, and D. C Woods Tumor necrosis factor-related apoptosis inducing ligand expression and activity in hen granulosa cells Reproduction, March 1, 2007; 133(3): 609 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Orisaka, S. Orisaka, J.-Y. Jiang, J. Craig, Y. Wang, F. Kotsuji, and B. K. Tsang Growth Differentiation Factor 9 Is Antiapoptotic during Follicular Development from Preantral to Early Antral Stage Mol. Endocrinol., October 1, 2006; 20(10): 2456 - 2468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.T. Bridgham and A.L. Johnson Alternatively Spliced Variants of Gallus gallus TNFRSF23 Are Expressed in the Ovary and Differentially Regulated by Cell Signaling Pathways Biol Reprod, April 1, 2004; 70(4): 972 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.M. Perks, P.V. Newcomb, M. Grohmann, R.J. Wright, H.D. Mason, and J.M.P. Holly Prolactin acts as a potent survival factor against C2-ceramide-induced apoptosis in human granulosa cells Hum. Reprod., December 1, 2003; 18(12): 2672 - 2677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Siskind, A. Davoody, N. Lewin, S. Marshall, and M. Colombini Enlargement and Contracture of C2-Ceramide Channels Biophys. J., September 1, 2003; 85(3): 1560 - 1575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Pru, I. R. Hendry, J. S. Davis, and B. R. Rueda Soluble Fas Ligand Activates the Sphingomyelin Pathway and Induces Apoptosis in Luteal Steroidogenic Cells Independently of Stress-Activated p38MAPK Endocrinology, November 1, 2002; 143(11): 4350 - 4357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Johnson, J. S. Langer, and J. T. Bridgham Survivin as a Cell Cycle-Related and Antiapoptotic Protein in Granulosa Cells Endocrinology, September 1, 2002; 143(9): 3405 - 3413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Siskind, R. N. Kolesnick, and M. Colombini Ceramide Channels Increase the Permeability of the Mitochondrial Outer Membrane to Small Proteins J. Biol. Chem., July 19, 2002; 277(30): 26796 - 26803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Morrison and J. L. Marcinkiewicz Tumor Necrosis Factor {alpha} Enhances Oocyte/Follicle Apoptosis in the Neonatal Rat Ovary Biol Reprod, February 1, 2002; 66(2): 450 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.T. Bridgham and A.L. Johnson Expression and Regulation of Fas Antigen and Tumor Necrosis Factor Receptor Type I in Hen Granulosa Cells Biol Reprod, September 1, 2001; 65(3): 733 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Soboloff, H. Sasaki, and B.K. Tsang Follicular Stage-Dependent Tumor Necrosis Factor {alpha}-Induced Hen Granulosa Cell Integrin Production and Survival in the Presence of Transforming Growth Factor {alpha} In Vitro Biol Reprod, August 1, 2001; 65(2): 477 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. MORITA and J. L. TILLY Sphingolipid Regulation of Female Gonadal Cell Apoptosis Ann. N.Y. Acad. Sci., April 1, 2000; 905(1): 209 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.L. Johnson and J.T. Bridgham Caspase-3 and -6 Expression and Enzyme Activity in Hen Granulosa Cells Biol Reprod, March 1, 2000; 62(3): 589 - 598. [Abstract] [Full Text]
|
|
|
|
|
|
S. L. Vickers, R. G. Cowan, R. M. Harman, D. A. Porter, and S. M. Quirk Expression and Activity of the Fas Antigen in Bovine Ovarian Follicle Cells Biol Reprod, January 1, 2000; 62(1): 54 - 61. [Abstract] [Full Text]
|
|
|
|
|
|
S. K. Balchak and J. L. Marcinkiewicz Evidence for the Presence of Tumor Necrosis Factor Alpha Receptors During Ovarian Development in the Rat Biol Reprod, December 1, 1999; 61(6): 1506 - 1512. [Abstract] [Full Text]
|
|
|
|
|
|
A. L. Johnson, J. T. Bridgham, and T. Jensen Bcl-XLONG Protein Expression and Phosphorylation in Granulosa Cells Endocrinology, October 1, 1999; 140(10): 4521 - 4529. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Garzotto, A. Haimovitz-Friedman, W.-C. Liao, M. White-Jones, R. Huryk, W. D. W. Heston, C. Cardon-Cardo, R. Kolesnick, and Z. Fuks Reversal of Radiation Resistance in LNCaP Cells by Targeting Apoptosis through Ceramide Synthase Cancer Res., October 1, 1999; 59(20): 5194 - 5201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-C. Liao, A. Haimovitz-Friedman, R. S. Persaud, M. McLoughlin, D. Ehleiter, N. Zhang, M. Gatei, M. Lavin, R. Kolesnick, and Z. Fuks Ataxia Telangiectasia-mutated Gene Product Inhibits DNA Damage-induced Apoptosis via Ceramide Synthase J. Biol. Chem., June 18, 1999; 274(25): 17908 - 17917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Soboloff, A. Sorisky, M. Desilets, and B.K. Tsang Acyl Chain Length-Specific Ceramide-Induced Changes in Intracellular Ca2+ Concentration and Progesterone Production Are Not Regulated by Tumor Necrosis Factor {alpha} in Hen Granulosa Cells Biol Reprod, February 1, 1999; 60(2): 262 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Quirk, D. A. Porter, S. C. Huber, and R. G. Cowan Potentiation of Fas-Mediated Apoptosis of Murine Granulosa Cells by Interferon-{gamma}, Tumor Necrosis Factor-{alpha}, and Cycloheximide Endocrinology, December 1, 1998; 139(12): 4860 - 4869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Aten, T. R. Kolodecik, M. J. Rossi, C. Debusscher, and H. R. Behrman Prostaglandin F2{alpha} Treatment In Vivo, but Not In Vitro, Stimulates Protein Kinase C-Activated Superoxide Production by Nonsteroidogenic Cells of the Rat Corpus Luteum Biol Reprod, November 1, 1998; 59(5): 1069 - 1076. [Abstract] [Full Text]
|
|
|
|
|
|
R. Bose, P. Chen, A. Loconti, C. Grullich, J. M. Abrams, and R. N. Kolesnick Ceramide Generation by the Reaper Protein Is Not Blocked by the Caspase Inhibitor, p35 J. Biol. Chem., October 30, 1998; 273(44): 28852 - 28859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kaipia, S. Y. Hsu, and A. J. W. Hsueh Expression and Function of a Proapoptotic Bcl-2 Family Member Bcl-XL/Bcl-2-Associated Death Promoter (BAD) in Rat Ovary Endocrinology, December 1, 1997; 138(12): 5497 - 5504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. P. Gill, C. M. Perks, P. V. Newcomb, and J. M. P. Holly Insulin-like Growth Factor-binding Protein (IGFBP-3) Predisposes Breast Cancer Cells to Programmed Cell Death in a Non-IGF-dependent Manner J. Biol. Chem., October 10, 1997; 272(41): 25602 - 25607. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
