| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Endocrinology, Vol 137, 3649-3658, Copyright © 1996 by Endocrine Society
ARTICLES |
YS Kim, PN MacDonald, S Dedhar and KA Hruska
Department of Pharmacological and Physiological Science, St. Louis University School of Medicine, Missouri, USA.
We previously reported nongenomic activation of ROS 17/2.8 cells by vitamin D metabolites (1 alpha,25-dihydroxyvitamin D3 [1 alpha,25- (OH)2D3], 25-hydroxyvitamin D3, 22-oxa-calcitriol, etc.). The hormone 1 alpha,25-(OH)2D3, or calcitriol, mediated rapid transient changes in intracellular free calcium levels and concomitant stimulation of inositol polyphosphate and diacylglycerol production. These effects resemble the mechanism of cell activation induced by ligands with plasma membrane (PM) receptors. As preliminary studies indicated that PM isolated from ROS 17/2.8 cells lacked specific binding sites for calcitriol alone, we studied the association between calcitriol- occupied vitamin D receptors (VDR) and ROS 17/2.8 cellular membranes. Saturable binding to the PM and the endoplasmic reticulum (ER) of calcitriol-occupied VDR was demonstrated. Binding of the VDR- [3H]calcitriol complex was displaceable by nonradioactive VDR/calcitriol, but not by the unoccupied VDR or by calcitriol alone. ER binding, but not PM binding, was competitively inhibited by a peptide from the VDR sequence recognized by an ER protein, calreticulin, and by an anticalreticulin antibody. The monoclonal antibody (9A7) against the VDR inhibited PM and ER binding of the hormone-occupied VDR. These results were substantiated by studies using baculovirus-expressed human VDR for binding studies with the PM and ER and for immunoblot analysis. We conclude that specific PM and ER sites of association for calcitriol-occupied VDR exist and suggest that these associations could participate in the nongenomic rapid actions of 1 alpha,25-(OH)2D3.
This article has been cited by other articles:
 |
|
 |
|
  S. Masia, S. Alvarez, A. R. de Lera, and D. Barettino Rapid, Nongenomic Actions of Retinoic Acid on Phosphatidylinositol-3-Kinase Signaling Pathway Mediated by the Retinoic Acid Receptor Mol. Endocrinol., October 1, 2007; 21(10): 2391 - 2402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ochiai, D. Miura, H. Eguchi, S. Ohara, K. Takenouchi, Y. Azuma, T. Kamimura, A. W. Norman, and S. Ishizuka Molecular Mechanism of the Vitamin D Antagonistic Actions of (23S)-25-Dehydro-1{alpha}-Hydroxyvitamin D3-26,23-Lactone Depends on the Primary Structure of the Carboxyl-Terminal Region of the Vitamin D Receptor Mol. Endocrinol., May 1, 2005; 19(5): 1147 - 1157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Huhtakangas, C. J. Olivera, J. E. Bishop, L. P. Zanello, and A. W. Norman The Vitamin D Receptor Is Present in Caveolae-Enriched Plasma Membranes and Binds 1{alpha},25(OH)2-Vitamin D3 in Vivo and in Vitro Mol. Endocrinol., November 1, 2004; 18(11): 2660 - 2671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Mizwicki, D. Keidel, C. M. Bula, J. E. Bishop, L. P. Zanello, J.-M. Wurtz, D. Moras, and A. W. Norman Identification of an alternative ligand-binding pocket in the nuclear vitamin D receptor and its functional importance in 1{alpha},25(OH)2-vitamin D3 signaling PNAS, August 31, 2004; 101(35): 12876 - 12881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-M. Nguyen, M. Lieberherr, J. Fritsch, H. Guillozo, M. L. Alvarez, Z. Fitouri, F. Jehan, and M. Garabedian The Rapid Effects of 1,25-Dihydroxyvitamin D3 Require the Vitamin D Receptor and Influence 24-Hydroxylase Activity: STUDIES IN HUMAN SKIN FIBROBLASTS BEARING VITAMIN D RECEPTOR MUTATIONS J. Biol. Chem., February 27, 2004; 279(9): 7591 - 7597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. J. HOENDEROP, O. DARDENNE, M. VAN ABEL, A. W. C. M. VAN DER KEMP, C. H. VAN OS, R. ST.-ARNAUD, and R. J. M. BINDELS Modulation of renal Ca2+ transport protein genes by dietary Ca2+ and 1,25-dihydroxyvitamin D3 in 25-hydroxyvitamin D3-1{alpha}-hydroxylase knockout mice FASEB J, September 1, 2002; 16(11): 1398 - 1406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Nemere, D. Larsson, and K. Sundell A specific binding moiety for 1,25-dihydroxyvitamin D3 in basal lateral membranes of carp enterocytes Am J Physiol Endocrinol Metab, September 1, 2000; 279(3): E614 - E621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Crescioli, M. Maggi, G. B. Vannelli, M. Luconi, R. Salerno, T. Barni, M. Gulisano, G. Forti, and M. Serio Effect of a Vitamin D3 Analogue on Keratinocyte Growth Factor-Induced Cell Proliferation in Benign Prostate Hyperplasia J. Clin. Endocrinol. Metab., July 1, 2000; 85(7): 2576 - 2583. [Abstract] [Full Text]
|
|
|
|
|
|
I. Nemere, R. Ray, and W. McManus Immunochemical studies on the putative plasmalemmal receptor for 1,25(OH)2D3. I. Chick intestine Am J Physiol Endocrinol Metab, June 1, 2000; 278(6): E1104 - E1114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Racz and J. Barsony Hormone-dependent Translocation of Vitamin D Receptors Is Linked to Transactivation J. Biol. Chem., July 2, 1999; 274(27): 19352 - 19360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schroder, O. Hattenhauer, and G. Breves Phosphate Transport in Pig Proximal Small Intestines during Postnatal Development: Lack of Modulation by Calcitriol Endocrinology, April 1, 1998; 139(4): 1500 - 1507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Le Mellay, B. Grosse, and M. Lieberherr Phospholipase C beta and Membrane Action of Calcitriol and Estradiol J. Biol. Chem., May 2, 1997; 272(18): 11902 - 11907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Vazquez, A. R. de Boland, and R. L. Boland Involvement of Calmodulin in 1alpha ,25-Dihydroxyvitamin D3 Stimulation of Store-operated Ca2+ Influx in Skeletal Muscle Cells J. Biol. Chem., May 19, 2000; 275(21): 16134 - 16138. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
