help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moriscot, A. S.
Right arrow Articles by Dillmann, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moriscot, A. S.
Right arrow Articles by Dillmann, W. H.
Endocrinology Vol. 138, No. 1 26-32
Copyright © 1997 by The Endocrine Society

ARTICLES

Transcription of the Rat Sarcoplasmic Reticulum Ca2+ Adenosine Triphosphatase Gene Is Increased by 3,5,3'-Triiodothyronine Receptor Isoform-Specific Interactions with the Myocyte-Specific Enhancer Factor-2a1

Anselmo S. Moriscot2, M. Richard Sayen, Ronald Hartong, Patricia Wu and Wolfgang H. Dillmann

Department of Medicine, Division of Endocrinology and Metabolism, University of California-San Diego, La Jolla, California 92093-0618; and the Department of Internal Medicine, University of Heidelberg (R.H.), Heidelberg, Germany

Thyroid hormone (T3) increases the transcription of the sarcoplasmic reticulum Ca2+ adenosine triphosphatase (ATPase) gene (SERCA 2) through three thyroid hormone response elements. The existence of repetitive cis elements with different configurations is likely to serve specific functions such as interactions with nuclear transcription factors. In addition, the presence of different T3 receptor isoforms (T3Rs) may contribute to another level of complexity in providing specificity for T3 action. In this study, we investigated T3R{alpha}1- vs. T3Rß1-specific interactions with the myocyte enhancer-specific factor-2 (MEF-2) on the expression of the SERCA 2 gene in transient transfection assays in embryonal heart-derived H9c2 cells. MEF-2a in combination with either T3R{alpha}1 or T3Rß1 isoforms resulted in a 2.5-fold increase in SERCA 2 transgene expression in the absence of T3. Addition of T3 did not induce any further increase in SERCA 2 expression when T3R{alpha}1 and MEF-2a expression vectors were cotransfected. In contrast, in the presence of T3Rß1 and MEF-2, the addition of T3 increased chlorampenicol acetyltransferase activity by an additional 2.2-fold to a total 5.5-fold increase. The interaction between MEF-2a and T3R is transcription factor specific because another factor that binds to MEF-2 consensus sites (heart factor 1b) was not able to interact with T3R. In addition, MEF-2a failed to interact with other nuclear factors (cAMP response element-binding protein and Egr-1) that stimulate SERCA 2 gene transcription. In addition, we found that a single homologous thyroid hormone response element is not able to mediate the interactions between MEF-2a and T3Rs to increase SERCA 2 gene transcription. Our findings point to T3R isoform-specific interactions with a cell type-specific transcription factor (MEF-2) in the regulation of SERCA 2 gene expression.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Jiang, A. Xu, and N. Narayanan
Thyroid hormone downregulates the expression and function of sarcoplasmic reticulum-associated CaM kinase II in the rabbit heart
Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1384 - H1394.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
I Stoykov, B Zandieh-Doulabi, A F M Moorman, V Christoffels, W M Wiersinga, and O Bakker
Expression pattern and ontogenesis of thyroid hormone receptor isoforms in the mouse heart.
J. Endocrinol., May 1, 2006; 189(2): 231 - 245.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Xu, C. Liu, J. C. Clark, and J. A. Whitsett
Functional Genomic Responses to Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) and CFTR{Delta}508 in the Lung
J. Biol. Chem., April 21, 2006; 281(16): 11279 - 11291.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. Gloss, G. Giannocco, E. A. Swanson, A. S. Moriscot, G. Chiellini, T. Scanlan, J. D. Baxter, and W. H. Dillmann
Different Configurations of Specific Thyroid Hormone Response Elements Mediate Opposite Effects of Thyroid Hormone and GC-1 on Gene Expression
Endocrinology, November 1, 2005; 146(11): 4926 - 4933.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
K. Kinugawa, M. Y. Jeong, M. R. Bristow, and C. S. Long
Thyroid Hormone Induces Cardiac Myocyte Hypertrophy in a Thyroid Hormone Receptor {alpha}1-Specific Manner that Requires TAK1 and p38 Mitogen-Activated Protein Kinase
Mol. Endocrinol., June 1, 2005; 19(6): 1618 - 1628.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
R. Vlasblom, A. Muller, R. J.P Musters, M. J Zuidwijk, C. van Hardeveld, W. J Paulus, and W. S Simonides
Contractile arrest reveals calcium-dependent stimulation of SERCA2a mRNA expression in cultured ventricular cardiomyocytes
Cardiovasc Res, August 15, 2004; 63(3): 537 - 544.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
E. S. Bachman, T. G. Hampton, H. Dhillon, I. Amende, J. Wang, J. P. Morgan, and A. N. Hollenberg
The Metabolic and Cardiovascular Effects of Hyperthyroidism Are Largely Independent of {beta}-Adrenergic Stimulation
Endocrinology, June 1, 2004; 145(6): 2767 - 2774.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Clark, P. M. McDonough, E. Swanson, S. U. Trost, M. Suzuki, M. Fukuda, and W. H. Dillmann
Diabetes and the Accompanying Hyperglycemia Impairs Cardiomyocyte Calcium Cycling through Increased Nuclear O-GlcNAcylation
J. Biol. Chem., November 7, 2003; 278(45): 44230 - 44237.
[Abstract] [Full Text] [PDF]

Home page
 Eur. J. Cardiothorac. Surg.Home page
A. G. Cerillo, S. Bevilacqua, S. Storti, M. Mariani, E. Kallushi, A. Ripoli, A. Clerico, and M. Glauber
Free triiodothyronine: a novel predictor of postoperative atrial fibrillation
Eur. J. Cardiothorac. Surg., October 1, 2003; 24(4): 487 - 492.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Hadri, A. Ozog, F. Soncin, and A.-M. Lompre
Basal Transcription of the Mouse Sarco(endo)plasmic Reticulum Ca2+-ATPase Type 3 Gene in Endothelial Cells Is Controlled by Ets-1 and Sp1
J. Biol. Chem., September 20, 2002; 277(39): 36471 - 36478.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
P. Razeghi, M. E. Young, T. C. Cockrill, O. H. Frazier, and H. Taegtmeyer
Downregulation of Myocardial Myocyte Enhancer Factor 2C and Myocyte Enhancer Factor 2C-Regulated Gene Expression in Diabetic Patients With Nonischemic Heart Failure
Circulation, July 23, 2002; 106(4): 407 - 411.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Jiang, A. Xu, S. Tokmakejian, and N. Narayanan
Thyroid hormone-induced overexpression of functional ryanodine receptors in the rabbit heart
Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1429 - H1438.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. G. Peters, H. Mitchell-Felton, and S. C. Kandarian
Unloading induces transcriptional activation of the sarco(endo)plasmic reticulum Ca2+-ATPase 1 gene in muscle
Am J Physiol Cell Physiol, May 1, 1999; 276(5): C1218 - C1225.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
K. Kinugawa, K. Yonekura, R. C.J. Ribeiro, Y. Eto, T. Aoyagi, J. D. Baxter, S. A. Camacho, M. R. Bristow, C. S. Long, and P. C. Simpson
Regulation of Thyroid Hormone Receptor Isoforms in Physiological and Pathological Cardiac Hypertrophy
Circ. Res., September 28, 2001; 89(7): 591 - 598.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society