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Human Insulin-Like Growth Factor I (IGF-I) Produced in the Mammary Glands of Transgenic Rabbits: Yield, Receptor Binding, Mitogenic Activity, and Effects on IGF-Binding Proteins

Lehrstuhl für Molekulare Tierzucht und Haustiergenetik (E.W.) and Medizinische Klinik II, Klinikum Großhadern (M.M.W.), Ludwig-Maximilians-Universität, D-81377 München, Germany; Abteilung Innere Medizin II, Sektion Nephrologie, Medizinische Klinik und Poliklinik, Universität Ulm (P.M.J.), D-89081 Ulm, Germany; Forschungszentrum für Milch und Lebensmittel Weihenstephan, Technische Universität München (H.S., A.D.), D-85350 Freising-Weihenstephan, Germany; Research Center for Developmental Medicine and Biology, School of Medicine, University of Auckland (B.H.B.), Auckland, New Zealand; Abteilung Biotechnologie in der Tierproduktion, Interuniversitäres Forschungsinstitut für Agrarbiotechnologie (U.B., G.B.), A-3430 Tulln, Austria; and University of Veterinary Science (L.F.), H-1400 Budapest, Hungary

Address all correspondence and requests for reprints to: Dr. Eckhard Wolf, Lehrstuhl für Molekulare Tierzucht und Haustiergenetik, Ludwig-Maximilians-Universität, Feodor-Lynen-Str. 25, D-81377 München, Germany. E-mail: ewolf{at}lmb.uni-muenchen.de

Insulin-like growth factor I (IGF-I) has acute insulin-like metabolic effects and long-term anabolic actions offering a range of important therapeutic applications. To evaluate a system for large-scale production of this peptide in the mammary glands of transgenic livestock, we generated transgenic rabbits carrying fusion genes in which a synthetic DNA coding for human IGF-I (hIGF-I) was placed under the transcriptional control of regulatory elements isolated from the bovine _S1-casein (_S1-cas) gene. Western blot analysis of milk from _S1-cas-hIGF-I transgenic rabbits demonstrated production of high amounts of mature hIGF-I peptide (7.6 kDa). Quantitative analysis by RIA revealed hIGF-I levels between 50 and 300 µg/ml milk. Recombinant hIGF-I purified from the milk of _S1-cas-hIGF-I transgenic rabbits bound to IGF-I receptors on human IM-9 lymphoblasts and stimulated DNA synthesis by growth-arrested MG-63 human osteosarcoma cells as efficiently as hIGF-I produced in Escherichia coli. Ligand blot analysis of milk serum revealed the presence of 45-kDa, 30-kDa, and 23-kDa IGF-binding proteins (IGFBPs). The 30-kDa IGFBP was shown to be IGFBP-2 by immunoprecipitation using an antiserum raised against human IGFBP-2. Secretion of IGFBP-2 was markedly stimulated by hIGF-I overproduction in _S1-cas-hIGF-I transgenic rabbits. The latter displayed slightly increased milk yield, but no significant changes in total protein content or overall milk protein composition, and reared their offspring without any problems or clinical signs of impaired welfare, even after multiple lactations. Our results indicate that high amounts of biologically active hIGF-I can be produced in the mammary glands of _S1-cas-hIGF-I transgenic rabbits. Local production of hIGF-I in mammary tissue is associated with increased secretion of IGFBP-2, which may prevent major biological effects by high levels of hIGF-I on the mammary gland.

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