This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kol, S. Articles by Adashi, E. Y. Search for Related Content PubMed PubMed Citation Articles by Kol, S. Articles by Adashi, E. Y.

Interleukin-1ß Stimulates Ovarian Phospholipase A₂ (PLA₂) Expression and Activity: Up-Regulation of Both Secretory and Cytosolic PLA₂¹

Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, University of Maryland School of Medicine, Baltimore, Maryland 21201

Address all correspondence and requests for reprints to: Dr. Eli Y. Adashi, Departments of Obstetrics/Gynecology and Physiology, University of Maryland School of Medicine, 655 West Baltimore Street, BRB 11–010, Baltimore, Maryland, 21201. E-mail: eadashi{at}umabnet.ab.umd.edu

Interleukin (IL)-1ß has been shown to stimulate ovarian prostaglandin biosynthesis. We hypothesized that this effect entails the induction of phospholipase A₂ (PLA₂). Treatment of cultured whole ovarian dispersates of immature rat origin with IL-1ß produced significant increases in [³H]arachidonic acid (AA) release and [³H]prostanoid accumulation as well as increases in cellular PLA₂ activity and in secretory PLA₂ and cytosolic PLA₂ transcripts. Cotreatment with IL-1 receptor antagonist reversed IL-mediated (and basal) release of [³H]labeled AA and prostaglandin products, as well as cellular PLA₂ activity. Treatment with IL-1ß also promoted a significant decrease in the cellular content of [³H]phospholipids (apparently phosphatidylethanolamine but not phosphatidylcholine). These observations establish the ovary as a site of IL-1-dependent sPLA₂ and cPLA₂ gene expression, document the presence of a possible phosphatidylethanolamine-dependent PLA₂ activity in cultured whole ovarian dispersates, reveal the up-regulatory, receptor-mediated action of IL-1ß in this regard and suggest the existence of endogenous PLA₂-stimulating/IL-1-like bioactivity.

This article has been cited by other articles:

				D. M. Duffy, C. L. Seachord, and B. L. Dozier An Ovulatory Gonadotropin Stimulus Increases Cytosolic Phospholipase A2 Expression and Activity in Granulosa Cells of Primate Periovulatory Follicles J. Clin. Endocrinol. Metab., October 1, 2005; 90(10): 5858 - 5865. [Abstract] [Full Text] [PDF]

				J. Saito, M. Ando, D. Sussman, H. Negishi, G. King, and E. Y. Adashi Interleukin 1 Upregulates Ovarian Prostaglandin Endoperoxide Synthase-2 Expression: Evidence for Prostaglandin-Dependent/Ceramide-Independent Transcriptional Stimulation and for Message Stabilization Biol Reprod, December 1, 2001; 65(6): 1759 - 1765. [Abstract] [Full Text] [PDF]

				K. W. Chung, M. Ando, and E. Y. Adashi Periovulatory and Interleukin (IL)-1--Dependent Reglation of IL-6 in the Immature Rat Ovary: A Specific IL-1 Receptor-Mediated Eicosanoid-Dependent Effect Reproductive Sciences, September 1, 2000; 7(5): 301 - 308. [Abstract] [PDF]

				J. V. BONVENTRE The 85-kD Cytosolic Phospholipase A2 Knockout Mouse: A NewTool for Physiology and Cell Biology J. Am. Soc. Nephrol., February 1, 1999; 10(2): 404 - 412. [Full Text]

				M. D. Rosenthal, E. D. Albrecht, and G. J. Pepe Developmental Maturation of Baboon Placental Trophoblast: Expression of Messenger Ribonucleic Acid and Protein Levels of Cytosolic and Secretory Phospholipases A2 J. Clin. Endocrinol. Metab., August 1, 1998; 83(8): 2861 - 2867. [Abstract] [Full Text]

				M. Ando, S. Kol, E. Kokia, K. Ruutiainen-Altman, J. Sirois, R. M. Rohan, D. W. Payne, and E. Y. Adashi Rat Ovarian Prostaglandin Endoperoxide Synthase-1 and -2: Periovulatory Expression of Granulosa Cell-Based Interleukin-1-Dependent Enzymes Endocrinology, May 1, 1998; 139(5): 2501 - 2508. [Abstract] [Full Text] [PDF]