This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Navarro, L. Articles by Aceves, C. Search for Related Content PubMed PubMed Citation Articles by Navarro, L. Articles by Aceves, C.

Mammary Gland Type I Iodothyronine Deiodinase Is Encoded by a Short Messenger Ribonucleic Acid¹

Departamento de Neuroendocrinología, Centro de Neurobiología, Campus Juriquilla Queretaro,y Departmento de Microbiología y Parasitología, Facultad de Medicina, Mexico, D. F.,Universidad Nacional Autonoma de Mexico, Mexico

Address all correspondence and requests for reprints to: Dr. Luz Navarro, Centro de Neurobiologia, Universidad Nacional Autonoma de Mexico, Apartado Postal 1–1141, Queretaro 76001, Qro. Mexico. E-mail: lnavarro{at}servidor.unam.mx

Lactating rat mammary gland expresses a deiodinating activity that, on the basis of kinetic characteristics, corresponds to the so-called 5'-deiodinase type I (D1). In the present study we amplified and sequenced several D1 complementary DNA (cDNA) fragments from rat lactating mammary gland. The mammary cDNA was found to be identical to the previously reported rat liver cDNA in the coding region, but 465 nucleotides shorter on its 3'-untranslated region, suggesting that the D1 is the same in both tissues. D1 messenger RNA (mRNA) was also detected by reverse transcriptase-PCR in mammary glands from puberal and late pregnant rats, but not in virgin animals. Densitometric analysis showed a close and direct correlation between mRNA content and enzyme specific activity in mammary gland. Our results also show that rat liver contains both D1 mRNA forms and that the large form may respond to the thyroid status. These data suggest a differential and organ-specific expression of these mRNA forms, which could play a role in the functional regulation of D1 activity.

This article has been cited by other articles:

				S. Song and T. Oka Regulation of type II deiodinase expression by EGF and glucocorticoid in HC11 mouse mammary epithelium Am J Physiol Endocrinol Metab, June 1, 2003; 284(6): E1119 - E1124. [Abstract] [Full Text] [PDF]

				C. Aceves, O. Pineda, I. RamÍrez-C, M. de la Luz Navarro, and C. Valverde-R Mammary Type I Deiodinase Is Dependent on the Suckling Stimulus: Differential Role of Norepinephrine and Prolactin Endocrinology, July 1, 1999; 140(7): 2948 - 2953. [Abstract] [Full Text]