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Regulation of K⁺ Channels by Cell Contact in a Cloned Folliculo-Stellate Cell (TtT/GF)

Fourth Department of Internal Medicine (T.Y., H.F., T.F., N.Y.), University of Tokyo School of Medicine, Tokyo 112 Japan; and Department of Regulation Biology (K.I.), Faculty of Science, University of Saitama, Saitama 338 Japan

Address all correspondence and requests for reprints to: Naohide Yamashita, M.D., Ph.D., Fourth Department of Internal Medicine, Tokyo University Branch Hospital, 3–28-6 Medjirodai, Bunkyo-ku, Tokyo 112 Japan.

Membrane currents in a folliculo-stellate cell line (TtT/GF) were examined using the whole-cell clamp technique. The cultured cells were morphologically categorized as isolated spread cells, isolated round cells, contact spread cells, and contact round cells. A distinct outward current was observed in isolated spread cells, contact round cells, and contact spread cells, whereas the outward current detected in isolated round cells was barely perceivable. The reversal potentials of the outward tail current obeyed the Nernst equation, indicating that the outward current was carried by K⁺ ions. The activation and deactivation processes of the K⁺ current could be fitted by single exponential curves. 4-aminopyridine, tetraethylammonium, and Ba²⁺ inhibited the K⁺ current. The concentrations for half-maximal inhibition of these agents to block the K⁺ current were 0.2 mM, 0.8 mM, and 8 mM, respectively. The biophysical and pharmacological characteristics of the K⁺ current in TtT/GF cells were similar to those of the K⁺ current in glial and Schwann cells. According to the results of the present study, it was concluded that TtT/GF cells possessed outward K⁺ channels, characteristics of which were similar to those of the K⁺ channels in glial and Schwann cells, and that the amplitude of the K⁺ current in TtT/GF cells seemed to be regulated by the condition of the cell contact.

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