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Preputial Sebocyte 5-Reductase Isoform Specificity¹

The University of Chicago, Pritzker School of Medicine, Departments of Pediatrics (D.D., R.L.R.), Medicine (R.L.R.), and Biochemistry and Molecular Biology (S.L.), Chicago, Illinois 60637-1470

Address all correspondence and requests for reprints to: Robert L. Rosenfield, M.D., The University of Chicago, Pritzker School of Medicine, Department of Pediatrics and Children’s Research Foundation, 5841 South Maryland Avenue (MC-5053), Chicago, Illinois 60637-1470.

We determined whether the 5-reductase (5-R) isozyme specificity in rat preputial sebocytes is in accordance with that of sebaceous glands (type 1 isoform) or with that of other genital tract androgen-dependent organs (type 2). Immunostaining indicated expression of type 1 5-R in preputial epithelial cells in the gland as well as in culture. Northern blots and ribonuclease protection assays both showed the presence of the type 1 isozyme in preputial sebocytes, with little if any expression of the type 2 messenger RNA. Ribonuclease protection assay demonstrated that both homogenized preputial gland and freshly dispersed preputial cells express 2-fold more 5-R type 1 messenger RNA than does skin and 7-fold more than cultured preputial cells. Since cultured preputial cells do not fully differentiate in monolayer culture, these findings suggest that 5-R is increasingly expressed as sebaceous cells differentiate. The predominance of the type 1 isoform in preputial sebocytes is important because it indicates programming by a skin- and sebocyte-specific signal in the midst of the genital tract where the type 2 isoform otherwise predominates.

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