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Centre de Recherche en Reproduction Animale (J.S.) and Département de Pathologie et Microbiologie (M.D.), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada J2S 7C6
Address all correspondence and requests for reprints to: Dr. Jean Sirois, CRRA, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, Saint-Hyacinthe, Quebec, Canada J2S 7C6. E-mail: siroisje{at}ere.umontreal.ca
PGs are important mediators of the ovulatory process and prostaglandin
G/H synthase-2 (PGHS-2) is a key rate-limiting enzyme in the PG
biosynthetic pathway. To determine whether PGHS-2 is regulated in
equine follicles before ovulation and, if so, to characterize its time
course of induction, preovulatory follicles were isolated during
estrus, 0, 12, 24, 30, 33, 36, and 39 h after an ovulatory dose of
hCG (n = 5 follicles/time point). Cellular extracts were obtained
from preparations of follicle wall (theca interna with attached
granulosa cells), isolated granulosa cells, and theca interna and were
analyzed by Western blot using specific anti-PGHS antibodies.
Immunohistochemistry was used to characterize the in
situ localization of PGHS-2 protein in preovulatory follicles,
and follicular fluid concentrations of PGE2 and PGF were
determined. The results showed the induction of PGHS-2, but not PGHS-1,
in equine follicles before ovulation. The PGHS-2 protein (72,000 mol
wt) was undetectable 0, 12, and 24 h post-hCG, first became
apparent at 30 h, and reached maximal levels 39 h after hCG
treatment. The induction of follicular PGHS-2 was localized exclusively
in granulosa cells, and a pronounced staining was observed in the
perinuclear region. Follicular fluid concentrations of PGE2
and PGF were low and not different between 0–33 h, but levels were
increased at 36 and 39 h post-hCG (P < 0.01).
Thus, the time course of PGHS-2 induction in equine follicles (30 h
post-hCG) is clearly distinct from those previously observed in rat (4
h post-hCG) and bovine (18 h post-hCG) preovulatory follicles.
Interestingly, in all three species, the interval from PGHS-2 induction
to follicular rupture is highly conserved (
10 h). Therefore, the
progressively delayed expression of PGHS-2 in species with longer
ovulatory processes supports its role as a molecular determinant of the
species-specific length of the ovulatory process.
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B. J. Davis, D. E. Lennard, C. A. Lee, H. F. Tiano, S. G. Morham, W. C. Wetsel, and R. Langenbach Anovulation in Cyclooxygenase-2-Deficient Mice Is Restored by Prostaglandin E2 and Interleukin-1{beta} Endocrinology, June 1, 1999; 140(6): 2685 - 2695. [Abstract] [Full Text]
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 A. F. de Arriba, F. Cavalcanti, A. Miralles, Y. Bayón, A. Alonso, M. Merlos, J. García-Rafanell, and J. Forn Inhibition of Cyclooxygenase-2 Expression by 4-Trifluoromethyl Derivatives of Salicylate, Triflusal, and Its Deacetylated Metabolite, 2-Hydroxy-4-trifluoromethylbenzoic Acid Mol. Pharmacol., April 1, 1999; 55(4): 753 - 760. [Abstract] [Full Text]
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A. Kerban, D. Boerboom, and J. Sirois Human Chorionic Gonadotropin Induces an Inverse Regulation of Steroidogenic Acute Regulatory Protein Messenger Ribonucleic Acid in Theca Interna and Granulosa Cells of Equine Preovulatory Follicles Endocrinology, February 1, 1999; 140(2): 667 - 674. [Abstract] [Full Text]
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M. Mikuni, M. Pall, C.M. Peterson, C.A. Peterson, P. Hellberg, M. Brännström, J.S. Richards, and L. Hedin The Selective Prostaglandin Endoperoxide Synthase-2 Inhibitor, NS-398, Reduces Prostaglandin Production and Ovulation In Vivo and In Vitro in the Rat Biol Reprod, November 1, 1998; 59(5): 1077 - 1083. [Abstract] [Full Text]
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D. Boerboom and J. Sirois Molecular Characterization of Equine Prostaglandin G/H Synthase-2 and Regulation of Its Messenger Ribonucleic Acid in Preovulatory Follicles Endocrinology, April 1, 1998; 139(4): 1662 - 1670. [Abstract] [Full Text] [PDF]
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J. S. Richards Editorial: Sounding the Alarm--Does Induction of Prostaglandin Endoperoxide Synthase-2 Control the Mammalian Ovulatory Clock? Endocrinology, October 1, 1997; 138(10): 4047 - 4048. [Full Text] [PDF]
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F. Filion, N. Bouchard, A. K. Goff, J. G. Lussier, and J. Sirois Molecular Cloning and Induction of Bovine Prostaglandin E Synthase by Gonadotropins in Ovarian Follicles Prior to Ovulation in Vivo J. Biol. Chem., August 31, 2001; 276(36): 34323 - 34330. [Abstract] [Full Text] [PDF]
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