help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martin, I.
Right arrow Articles by Quarto, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martin, I.
Right arrow Articles by Quarto, R.
Endocrinology Vol. 138, No. 10 4456-4462
Copyright © 1997 by The Endocrine Society

ARTICLES

Fibroblast Growth Factor-2 Supports ex Vivo Expansion and Maintenance of Osteogenic Precursors from Human Bone Marrow1

Ivan Martin, Anita Muraglia2, Giuliano Campanile, Ranieri Cancedda and Rodolfo Quarto

Laboratorio di Differenziamento Cellulare (I.M., A.M., G.C., R.C., R.Q.), Istituto Nazionale per la Ricerca sul Cancro/Centro di Biotecnologie Avanzate, 16132 Genova, Italy; Dipartimento di Informatica (I.M.), Sistemistica e Telematica (DIST), Università di Genova, 16145 Genova, Italy; and Dipartimento di Oncologia Clinica e Sperimentale (R.C.), Università di Genova, 16132 Genova, Italy

Address all correspondence and requests for reprints to: Rodolfo Quarto M.D., Laboratorio di Differenziamento Cellulare, Istituto Nazionale per la Ricerca sul Cancro/Centro di Biotecnologie Avanzate, Largo Rosanna Benzi no. 10, 16132 Genova, Italy.

We have investigated the effects of different growth factors on the proliferation and osteogenic potential of primary cultures of human bone marrow stromal cells (BMSC). Fibroblast growth factor (FGF)-2 was the most effective in promoting growth of these cells in vitro. The size of colonies formed in clonal conditions was approximately 2.5 times larger in presence of FGF-2. Also the morphology of BMSC was affected: cells cultured in 10% FCS alone became flattened, whereas FGF-2 expanded cells maintained a fibroblast-like elongated phenotype. Levels of alkaline phosphatase activity in BMSC expanded with FGF-2 were significantly lower (56%) than control and, after stimulation with ascorbic acid, ßGlycerophosphate and dexamethasone, FGF-2 expanded BMSC deposited approximately 3-fold more mineralized matrix than control cells. We have assessed osteogenicity of BMSC on hydroxyapatite porous scaffolds (bioceramics) by an ectopic bone formation assay. FGF-2 expanded BMSC yielded a higher bone formation (>20-fold) than control cells. We conclude that FGF-2, promoting BMSC proliferation, maintains cells in a more immature state allowing in vitro expansion of human osteo-progenitors which, associated with bioceramics, can differentiate in vivo and form bone tissue.




This article has been cited by other articles:


Home page
 Stem CellsHome page
A. Scherberich, R. Galli, C. Jaquiery, J. Farhadi, and I. Martin
Three-Dimensional Perfusion Culture of Human Adipose Tissue-Derived Endothelial and Osteoblastic Progenitors Generates Osteogenic Constructs with Intrinsic Vascularization Capacity
Stem Cells, July 1, 2007; 25(7): 1823 - 1829.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
L.-E. Zaragosi, G. Ailhaud, and C. Dani
Autocrine Fibroblast Growth Factor 2 Signaling Is Critical for Self-Renewal of Human Multipotent Adipose-Derived Stem Cells
Stem Cells, November 1, 2006; 24(11): 2412 - 2419.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. A. Dupree, S. R. Pollack, E. M. Levine, and C. T. Laurencin
Fibroblast Growth Factor 2 Induced Proliferation in Osteoblasts and Bone Marrow Stromal Cells: A Whole Cell Model
Biophys. J., October 15, 2006; 91(8): 3097 - 3112.
[Abstract] [Full Text] [PDF]

Home page
 JBJSHome page
J. I. Huang, M. M. Durbhakula, P. Angele, B. Johnstone, and J. U. Yoo
Lunate Arthroplasty with Autologous Mesenchymal Stem Cells in a Rabbit Model
J. Bone Joint Surg. Am., April 1, 2006; 88(4): 744 - 752.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
Z. Guo, H. Li, X. Li, X. Yu, H. Wang, P. Tang, and N. Mao
In Vitro Characteristics and In Vivo Immunosuppressive Activity of Compact Bone-Derived Murine Mesenchymal Progenitor Cells
Stem Cells, April 1, 2006; 24(4): 992 - 1000.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
W. Kafienah, S. Mistry, C. Williams, and A. P. Hollander
Nucleostemin Is a Marker of Proliferating Stromal Stem Cells in Adult Human Bone Marrow
Stem Cells, April 1, 2006; 24(4): 1113 - 1120.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
P. A. Sotiropoulou, S. A. Perez, M. Salagianni, C. N. Baxevanis, and M. Papamichail
Characterization of the Optimal Culture Conditions for Clinical Scale Production of Human Mesenchymal Stem Cells
Stem Cells, February 1, 2006; 24(2): 462 - 471.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
H. L. Wamsley, U. T. Iwaniec, and T. J. Wronski
Selected Extraskeletal Effects of Systemic Treatment with Basic Fibroblast Growth Factor in Ovariectomized Rats
Toxicol Pathol, August 1, 2005; 33(5): 577 - 583.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. F. Pittenger and B. J. Martin
Mesenchymal Stem Cells and Their Potential as Cardiac Therapeutics
Circ. Res., July 9, 2004; 95(1): 9 - 20.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. Saverino, F. Ghiotto, A. Merlo, S. Bruno, L. Battini, M. Occhino, M. Maffei, C. Tenca, S. Pileri, L. Baldi, et al.
Specific Recognition of the Viral Protein UL18 by CD85j/LIR-1/ILT2 on CD8+ T Cells Mediates the Non-MHC-Restricted Lysis of Human Cytomegalovirus-Infected Cells
J. Immunol., May 1, 2004; 172(9): 5629 - 5637.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. L. Roccisana, N. Kawanabe, H. Kajiya, M. Koide, G. D. Roodman, and S. V. Reddy
Functional Role for Heat Shock Factors in the Transcriptional Regulation of Human RANK Ligand Gene Expression in Stromal/Osteoblast Cells
J. Biol. Chem., March 12, 2004; 279(11): 10500 - 10507.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
R. Cancedda, G. Bianchi, A. Derubeis, and R. Quarto
Cell Therapy for Bone Disease: A Review of Current Status
Stem Cells, September 1, 2003; 21(5): 610 - 619.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Muraglia, A. Corsi, M. Riminucci, M. Mastrogiacomo, R. Cancedda, P. Bianco, and R. Quarto
Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells
J. Cell Sci., July 15, 2003; 116(14): 2949 - 2955.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
L. Cheng, H. Hammond, Z. Ye, X. Zhan, and G. Dravid
Human Adult Marrow Cells Support Prolonged Expansion of Human Embryonic Stem Cells in Culture
Stem Cells, March 1, 2003; 21(2): 131 - 142.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
R. Quarto, M. Mastrogiacomo, R. Cancedda, S. M. Kutepov, V. Mukhachev, A. Lavroukov, E. Kon, and M. Marcacci
Repair of Large Bone Defects with the Use of Autologous Bone Marrow Stromal Cells
N. Engl. J. Med., February 1, 2001; 344(5): 385 - 386.
[Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A Muraglia, R Cancedda, and R Quarto
Clonal mesenchymal progenitors from human bone marrow differentiate in vitro according to a hierarchical model
J. Cell Sci., January 4, 2000; 113(7): 1161 - 1166.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
H. Liang, S. Pun, and T. J. Wronski
Bone Anabolic Effects of Basic Fibroblast Growth Factor in Ovariectomized Rats
Endocrinology, December 1, 1999; 140(12): 5780 - 5788.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society