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Endocrinology Vol. 138, No. 10 4481-4484
Copyright © 1997 by The Endocrine Society

ARTICLES

Human Neutrophils Express GH-N Gene Transcripts and the Pituitary Transcription Factor Pit-1b

R. Kooijman, D. Berus, A. Malur, M. Delhase and E. L. Hooghe-Peters

Department of Pharmacology, Medical School, Free University of Brussels (V.U.B.), Brussels, Belgium

Address all correspondence and requests for reprints to: Dr. Ron Kooijman, Free University of Brussels Medical School, Department of Pharmacology, Laarbieklaan 103, Brussels, B-1090 Belgium.

Since GH stimulates the development and function of granulocytes, we investigated the expression of GH in granulocyte subsets. By immunocytochemistry, 25 ± 7% of the human neutrophils were shown to express immunoreactive GH, whereas eosinophils were negative. Reversed transcription (RT)-PCR analysis demonstrated GH mRNA in neutrophils. Restriction analysis revealed that neutrophils express the GH-N gene but not the GH-V gene. Furthermore, we demonstrated by western blot analysis that neutrophils express an alternatively spliced variant of the pituitary transcription factor Pit-1, designated Pit-1b.




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Copyright © 1997 by The Endocrine Society