This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Van Wyk, J. J. Articles by Bruton, E. T. Search for Related Content PubMed PubMed Citation Articles by Van Wyk, J. J. Articles by Bruton, E. T.

Molecular Basis for Species and Ligand Specificity of a Monoclonal Antibody Raised Against Human IGF-I

Department of Pediatrics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599

Address all correspondence and requests for reprints to: Judson J. Van Wyk, M.D., University of North Carolina, Department of Pediatrics, School of Medicine, 509 Clinical Science Building, CB# 7220, Chapel Hill, North Carolina 27599.

The anti-hIGF-I monoclonal antibody, -sm1.2, was found to have substantial crossreactivity with human and rat IGF-II, but recognized rat IGF-I only when this ligand was present at very high concentration. (E₅₀ for hIGF-I 3.5 ng/tube vs. 12,000 ng/tube for rat IGF-I). In the context of previous studies to define the epitope(s) of -sm1.2, these findings point to the critical importance of aspartic acid at residue 20 in the B domain in determining the species and ligand specificity of this antibody. Previous studies using this antibody in rodent tissues may require reinterpretation in the light of these findings.

This article has been cited by other articles:

				B. P Hauffa, N. Lehmann, M. Bettendorf, O. Mehls, H.-G. Dorr, N. Stahnke, H. Steinkamp, E. Said, M. B Ranke, and participating members of the German KIGS Board/Med Central laboratory reassessment of IGF-I, IGF-binding protein-3, and GH serum concentrations measured at local treatment centers in growth-impaired children: implications for the agreement between outpatient screening and the results of somatotropic axis functional testing Eur. J. Endocrinol., November 1, 2007; 157(5): 597 - 603. [Abstract] [Full Text] [PDF]

				J. G. Simmons, J. B. Pucilowska, and P. K. Lund Autocrine and paracrine actions of intestinal fibroblast-derived insulin-like growth factors Am J Physiol Gastrointest Liver Physiol, April 1, 1999; 276(4): G817 - G827. [Abstract] [Full Text] [PDF]