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Endocrinology Vol. 138, No. 11 4757-4767
Copyright © 1997 by The Endocrine Society

ARTICLES

Molecular Cloning of Ovine and Bovine Type I Interferon Receptor Subunits from Uteri, and Endometrial Expression of Messenger Ribonucleic Acid for Ovine Receptors During the Estrous Cycle and Pregnancy1

Chun-Sheng Han, Nagappan Mathialagan, Stephen W. Klemann and R. Michael Roberts

Departments of Biochemistry (C.S.H., R.M.R), Animal Sciences (N.M., R.M.R), and Veterinary Pathobiology (R.M.R), University of Missouri, Columbia, Missouri 65211; and Department of Biology (S.W.K.), Rollins College, Winter Park, Florida 32789

Address all correspondence and requests for reprints to: R. Michael Roberts, 158 Animal Sciences Reseach Center, University of Missouri, Columbia, Missouri 65211. E-mail: michael_roberts{at}muccmail.missouri edu.

Interferon-{tau} (IFN-{tau}), a type I IFN structurally related to IFN-{alpha}, is regarded as the major antiluteolytic factor secreted by the conceptus of ruminant ungulate species before definitive trophoblast attachment and implantation. It mediates its effects by acting on the uterine endometrium, where it blunts the normal pulsatile production of PGF2{alpha}, presumably as a result of its binding to type I IFN receptors. In this study, we describe the complementary DNAs for the two known subunits, IFNAR1 and IFNAR2, of this receptor isolated from bovine and ovine endometrial complementary DNA libraries by homology cloning. Although there is extensive inferred amino acid sequence similarity between bovine and ovine IFNAR1 (92% identity) and between bovine and ovine IFNAR2 (88% identity), they have diverged extensively from the human receptor subunits (~67% and ~58% identity, respectively). Despite these differences in primary structure, the respective subunits from all three species are organized similarly in their extracellular and cytoplasmic regions, and the bovine and ovine subunits have each retained a number of polypeptide motifs implicated in signal transduction. These uterine receptors also appear not to be splice variants. The cloned ovine IFNAR1 subunit, for example, possesses the expected four extracellular SD100 domains of full-length bovine and huIFNAR1, and only the homologs of the so-called long form (huIFNAR2c) of human IFNAR2 have so far been identified. RT-PCR procedures indicate that the messenger RNA for both subunits are found, not only in endometrium, but in all other tissues examined except those of preimplantation conceptuses, which presumably cannot respond to the IFN-{tau} they produce. Quantitative RNase protection assays of ovine endometrial RNA show that the expression of neither subunit changes greatly during the estrous cycle or pregnancy. These data suggest that the type I IFN receptor, which is expressed by the endometrium and binds IFN-{tau}, is probably not a structurally unusual form.




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