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Sorbin in the Porcine Gastrointestinal Tract and Pancreas: An Immunocytochemical Analysis¹

INSERM U-45 (F.A.E.F., P.N., M.D.-V.), Ecole Pratique des Hautes Etudes (D.P.), and Laboratoire d’Anatomie Pathologique (F.B.), Hôpital Edouard Herriot, 69437 Lyon, and Laboratoire d’Histologie-Embryologie, Faculté de Médecine Lyon-Sud (P.L.), 69921 Oullins, France

Address all correspondence and requests for reprints to: Dr. D. Pansu, Ecole Pratique des Hautes Etudes, INSERM U-45, Hôpital Edouard Herriot, 69437 Lyon Cedex 03, France.

Sorbin is a 153-amino acid peptide that was initially discovered in the porcine duodenum. We have reported previously that this peptide regulates intestinal electrolyte transport and have described accumulation sites in the rat digestive tract. In the present study, we investigated the anatomical distribution and the site(s) of sorbin production in the porcine digestive tract using immunocytochemistry. The use of polyclonal antisera, which by cross-reaction studies were shown to be specific for different regions of the molecule, revealed a diversified distribution. Sorbin predominated in endocrine cells preferentially localized in the pyloric glands, duodenal crypts of Lieberkühn, and pancreatic islets; in the gastrointestinal tract, sorbin coexisted with Met-enkephalin or with substance P in a small fraction of serotonin-storing [enterochromaffin (ED)] cells, i.e. EC₂ cells and EC₁ cells, respectively; in the pancreas, sorbin coexisted with insulin in the ß-cells, also considered as serotonin-storing cells in the pig, and with EC cells in the exocrine pancreas. An enteric neuronal system containing sorbin was also reported. Our results demonstrate that sorbin is a component of the serotonin-storing cell type in the porcine gastrointestinal tract and pancreas, and suggest potential directions to investigate the functions of this new regulatory peptide.

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				F A. El Fadil-Nicol, F Berger, M Descroix-Vagne, and D Pansu Presence of sorbin in human digestive tract and endocrine digestive tumours Gut, February 1, 2000; 46(2): 182 - 190. [Abstract] [Full Text] [PDF]