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Endocrinology Vol. 138, No. 11 5067-5070
Copyright © 1997 by The Endocrine Society

ARTICLES

Differential Expression of Estrogen Receptors {alpha} and ß mRNA During Differentiation of Human Osteoblast SV-HFO Cells

Janine Arts, George G. J. M. Kuiper, Josine M. M. F. Janssen, Jan-Åke Gustafsson, Clemens W. G. M. Löwik, Huibert A. P. Pols and Johannes P. T. M. van Leeuwen

Department of Internal Medicine III, Erasmus University Medical School, 3000 DR Rotterdam, The Netherlands; part of Postgraduate School Molecular Medicine: Pathophysiology of Growth and Differentiation; Center for Biotechnology (G.G.J.M.K., J.-A.G.), Department of Biosciences, Karolinska Institute, Huddinge, Sweden; Department of Endocrinology (C.W.G.M.L.), Academic Hospital Leiden, The Netherlands

Address all correspondence and requests for reprints to: Dr. J. Arts, Erasmus University Rotterdam, Department of Internal Medicine III, Rm Bd 236, P.O. Box 1738, Rotterdam 3000 DR, The Netherlands.

Estrogens have been shown to be essential for maintaining a sufficiently high bone mineral density and ER{alpha} expression has been demonstrated in bone cells. Recently, a novel estrogen receptor, estrogen receptor ß (ERß) has been identified. Here we demonstrate that also ERß is expressed in human osteoblasts, and that ER{alpha} and ERß are differentially expressed during human osteoblast differentiation. ERß mRNA expression increased gradually during osteoblast culture, resulting in an average increase of 9.9 ± 5.3 fold (mean ± S.D., n = 3) at day 21 (mineralization phase) as compared to day 6 (proliferation phase). In contrast, ER{alpha} mRNA expression levels increased only slightly until day 10 (2.3 ± 1.7 fold) and then remained constant. The observed differential regulation of ER{alpha} and ß is suggestive for an additional functional role of ERß to ER{alpha} in bone metabolism.




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