| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
ARTICLES |
Division of Endocrinology, Department of Medicine, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908
Address all correspondence and requests for reprints to: Dr. D. J. Haisenleder, Division of Endocrinology, Department of Medicine, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908. E-mail: djh2q{at}virginia.edu
Previously, we have shown that intermittent calcium (Ca2+)
stimuli increase 
, LHß, and FSHß messenger RNAs (mRNAs), and
only LHß mRNA was increased by continuous Ca2+. As
gonadotropin subunit and GnRH receptor (GnRH-R) mRNAs are
differentially regulated by alterations in GnRH pulse interval, we
aimed to determine whether changes in the frequency of Ca2+
signals play a role in this effect. Cultured adult female rat pituitary
cells in perifusion were given pulses of the Ca2+ channel
activator BayK 8644 (10 µM; with 10 mM KCl in
the injectate), at intervals of 16, 60, or 180 min for 24 h
(vehicle pulses or 100 pM GnRH to controls). Pulsatile
Ca2+ influx stimulated a rise in all mRNAs examined
(P < 0.05 vs. vehicle controls);
however, optimal pulse intervals differed. and LHß mRNAs were
maximally stimulated by 16- or 60-min pulses (57% and 74% increases,
respectively), with 180-min pulses being less effective. In contrast,
FSHß and GnRH-R mRNAs were selectively stimulated by 180-min pulses
(51% and 41% increases, respectively). Pulsatile GnRH produced
similar increases in GnRH-R and subunit mRNAs (53–78%
vs. controls). These results reveal that alterations in
the frequency of Ca2+ signals can regulate gonadotrope gene
expression in a differential manner, producing effects similar to
previous findings for GnRH. Thus, intermittent increases in
intracellular Ca2+ may be an important step in the
transmission of GnRH pulse signals from the plasma membrane to the
gene.
This article has been cited by other articles:
 |
|
 |
|
  H. Kanasaki, G. Y. Bedecarrats, K.-Y. Kam, S. Xu, and U. B. Kaiser Gonadotropin-Releasing Hormone Pulse Frequency-Dependent Activation of Extracellular Signal-Regulated Kinase Pathways in Perifused L{beta}T2 Cells Endocrinology, December 1, 2005; 146(12): 5503 - 5513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L L Burger, D J Haisenleder, A C Dalkin, and J C Marshall Regulation of gonadotropin subunit gene transcription J. Mol. Endocrinol., December 1, 2004; 33(3): 559 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Haisenleder, L. L. Burger, K. W. Aylor, A. C. Dalkin, and J. C. Marshall Gonadotropin-Releasing Hormone Stimulation of Gonadotropin Subunit Transcription: Evidence for the Involvement of Calcium/Calmodulin-Dependent Kinase II (Ca/CAMK II) Activation in Rat Pituitaries Endocrinology, July 1, 2003; 144(7): 2768 - 2774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Haisenleder, H. A. Ferris, and M. A. Shupnik The Calcium Component of Gonadotropin-Releasing Hormone-Stimulated Luteinizing Hormone Subunit Gene Transcription Is Mediated by Calcium/Calmodulin-Dependent Protein Kinase Type II Endocrinology, June 1, 2003; 144(6): 2409 - 2416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yuen, E. Wurmbach, B. J. Ebersole, F. Ruf, R. L. Pfeffer, and S. C. Sealfon Coupling of GnRH Concentration and the GnRH Receptor-Activated Gene Program Mol. Endocrinol., June 1, 2002; 16(6): 1145 - 1153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.J. Haisenleder, L.J. Workman, L.L. Burger, K.W. Aylor, A.C. Dalkin, and J.C. Marshall Gonadotropin Subunit Transcriptional Responses to Calcium Signals in the Rat: Evidence for Regulation by Pulse Frequency Biol Reprod, December 1, 2001; 65(6): 1789 - 1793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Weck, A. C. Anderson, S. Jenkins, P. C. Fallest, and M. A. Shupnik Divergent and Composite Gonadotropin-Releasing Hormone-Responsive Elements in the Rat Luteinizing Hormone Subunit Genes Mol. Endocrinol., April 1, 2000; 14(4): 472 - 485. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. J. Blum, M. C. Reed, J. A. Janovick, and P. M. Conn A mathematical model quantifying GnRH-induced LH secretion from gonadotropes Am J Physiol Endocrinol Metab, February 1, 2000; 278(2): E263 - E272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Call and M. W. Wolfe Gonadotropin-Releasing Hormone Activates the Equine Luteinizing Hormone {beta} Promoter Through a Protein Kinase C/Mitogen-Activated Protein Kinase Pathway Biol Reprod, September 1, 1999; 61(3): 715 - 723. [Abstract] [Full Text]
|
|
|
|
|
|
G. Maya-Núñez and P. M. Conn Transcriptional Regulation of the Gonadotropin-Releasing Hormone Receptor Gene Is Mediated in Part by a Putative Repressor Element and by the Cyclic Adenosine 3',5'-Monophosphate Response Element Endocrinology, August 1, 1999; 140(8): 3452 - 3458. [Abstract] [Full Text]
|
|
|
|
|
|
J. L. Turgeon and D. W. Waring Androgen Modulation of Luteinizing Hormone Secretion by Female Rat Gonadotropes Endocrinology, April 1, 1999; 140(4): 1767 - 1774. [Abstract] [Full Text]
|
|
|
|
|
|
X. Lin and P. M. Conn Transcriptional Activation of Gonadotropin-Releasing Hormone (GnRH) Receptor Gene by GnRH: Involvement of Multiple Signal Transduction Pathways Endocrinology, January 1, 1999; 140(1): 358 - 364. [Abstract] [Full Text]
|
|
|
|
|
|
X. Lin and P. M. Conn Transcriptional Activation of Gonadotropin-Releasing Hormone (GnRH) Receptor Gene by GnRH and Cyclic Adenosine Monophosphate Endocrinology, September 1, 1998; 139(9): 3896 - 3902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Haisenleder, M. E. Cox, S. J. Parsons, and J. C. Marshall Gonadotropin-Releasing Hormone Pulses Are Required to Maintain Activation of Mitogen-Activated Protein Kinase: Role in Stimulation of Gonadotrope Gene Expression Endocrinology, July 1, 1998; 139(7): 3104 - 3111. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
