help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iwasaki, Y.
Right arrow Articles by Majzoub, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iwasaki, Y.
Right arrow Articles by Majzoub, J. A.
Endocrinology Vol. 138, No. 12 5266-5274
Copyright © 1997 by The Endocrine Society

ARTICLES

Positive and Negative Regulation of the Rat Vasopressin Gene Promoter1

Yasumasa Iwasaki, Yutaka Oiso, Hidehiko Saito and Joseph A. Majzoub

The Division of Endocrinology, Department of Medicine, Children’s Hospital, Harvard Medical School (Y.I., J.A.M), Boston, Massachusetts 02115; and the First Department of Internal Medicine, Nagoya University School of Medicine (Y.I., Y.O., H.S.), Nagoya 466, Japan

Address all correspondence and requests for reprints to: Joseph A. Majzoub, M.D., Division of Endocrinology, Children’s Hospital, 300 Longwood Avenue, Boston, Massachusetts 02115. E-mail: majzoub{at}a1.tch.havard.edu

To study the transcriptional regulation of the vasopressin gene in vitro, 3 kb of the 5' regulatory region of the rat vasopressin gene was isolated and subcloned, along with a series of various deletion mutants, into vectors containing the luciferase reporter gene. After transfecting these genes transiently into the human choriocarcinoma cell line JEG-3 along with a glucocorticoid receptor (GR) expression vector, transcriptional activity was quantitated using the luciferase assay. Forskolin, 8-bromo-cAMP, and protein kinase A catalytic subunit expression all markedly increased transcription from the 3-kb promoter. Analyses with deletion mutants of the promoter showed that two cAMP-responsive element (CRE)-like sequences (-227 to -220 bp and -123 to -116 bp) contribute to this positive regulation. Expression of KCREB, a dominant negative mutant of the cAMP-responsive element binding protein (CREB), suggested the involvement of CREB. Transfection of the activator protein 2 (AP2) DNA consensus sequence partially blocked transcription. Dexamethasone suppressed forskolin-stimulated expression. The negative effect of glucocorticoid was GR dependent and may be mediated by a mechanism not involving GR binding to DNA because it was independent of the putative glucocorticoid-responsive element previously reported in the vasopressin promoter (-622 to -608 bp) and was preserved in the shorter promoter constructs in which no glucocorticoid-responsive element-like sequence was found. Our data suggest that several trans-acting factors including CREB, AP2, and GR are likely to be involved in vasopressin gene transcription and that the positive and negative regulation of vasopressin gene transcription is complex.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
Y. Iwasaki, M. Nishiyama, T. Taguchi, M. Kambayashi, M. Asai, M. Yoshida, T. Nigawara, and K. Hashimoto
Activation of AMP-activated protein kinase stimulates proopiomelanocortin gene transcription in AtT20 corticotroph cells
Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1899 - E1905.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Yoshida, Y. Iwasaki, M. Asai, S. Takayasu, T. Taguchi, K. Itoi, K. Hashimoto, and Y. Oiso
Identification of a Functional AP1 Element in the Rat Vasopressin Gene Promoter
Endocrinology, June 1, 2006; 147(6): 2850 - 2863.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Nigawara, Y. Iwasaki, M. Asai, M. Yoshida, M. Kambayashi, H. Sashinami, K. Hashimoto, and T. Suda
Inhibition of 11{beta}-Hydroxysteroid Dehydrogenase Eliminates Impaired Glucocorticoid Suppression and Induces Apoptosis in Corticotroph Tumor Cells
Endocrinology, February 1, 2006; 147(2): 769 - 772.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
O. C. Meijer, B. Topic, P. J. Steenbergen, G. Jocham, J. P. Huston, and M. S. Oitzl
Correlations between Hypothalamus-Pituitary-Adrenal Axis Parameters Depend on Age and Learning Capacity
Endocrinology, March 1, 2005; 146(3): 1372 - 1381.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
E Yamamori, M Asai, M Yoshida, K Takano, K Itoi, Y Oiso, and Y Iwasaki
Calcium/calmodulin kinase IV pathway is involved in the transcriptional regulation of the corticotropin-releasing hormone gene promoter in neuronal cells
J. Mol. Endocrinol., December 1, 2004; 33(3): 639 - 649.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. Asai, Y. Iwasaki, M. Yoshida, N. Mutsuga-Nakayama, H. Arima, M. Ito, K. Takano, and Y. Oiso
Nuclear Factor of Activated T Cells (NFAT) Is Involved in the Depolarization-Induced Activation of Growth Hormone-Releasing Hormone Gene Transcription in Vitro
Mol. Endocrinol., December 1, 2004; 18(12): 3011 - 3019.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. Murgatroyd, A. Wigger, E. Frank, N. Singewald, M. Bunck, F. Holsboer, R. Landgraf, and D. Spengler
Impaired Repression at a Vasopressin Promoter Polymorphism Underlies Overexpression of Vasopressin in a Rat Model of Trait Anxiety
J. Neurosci., September 1, 2004; 24(35): 7762 - 7770.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. Asaba, Y. Iwasaki, M. Yoshida, M. Asai, Y. Oiso, T. Murohara, and K. Hashimoto
Attenuation by Reactive Oxygen Species of Glucocorticoid Suppression on Proopiomelanocortin Gene Expression in Pituitary Corticotroph Cells
Endocrinology, January 1, 2004; 145(1): 39 - 42.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
E. Yamamori, Y. Iwasaki, Y. Oki, M. Yoshida, M. Asai, M. Kambayashii, Y. Oiso, and N. Nakashima
Possible Involvement of Ryanodine Receptor-Mediated Intracellular Calcium Release in the Effect of Corticotropin-Releasing Factor on Adrenocorticotropin Secretion
Endocrinology, January 1, 2004; 145(1): 36 - 38.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Yoshida, Y. Iwasaki, M. Asai, T. Nigawara, and Y. Oiso
Gene Therapy for Central Diabetes Insipidus: Effective Antidiuresis by Muscle-Targeted Gene Transfer
Endocrinology, January 1, 2004; 145(1): 261 - 268.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Kuwahara, H. Arima, R. Banno, I. Sato, N. Kondo, and Y. Oiso
Regulation of Vasopressin Gene Expression by cAMP and Glucocorticoids in Parvocellular Neurons of the Paraventricular Nucleus in Rat Hypothalamic Organotypic Cultures
J. Neurosci., November 12, 2003; 23(32): 10231 - 10237.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
T. Mune, H. Murase, N. Yamakita, T. Fukuda, M. Murayama, A. Miura, T. Suwa, J. Hanafusa, H. Daido, H. Morita, et al.
Eutopic Overexpression of Vasopressin V1a Receptor in Adrenocorticotropin-Independent Macronodular Adrenal Hyperplasia
J. Clin. Endocrinol. Metab., December 1, 2002; 87(12): 5706 - 5713.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Arima, S. B. House, H. Gainer, and G. Aguilera
Neuronal Activity Is Required for the Circadian Rhythm of Vasopressin Gene Transcription in the Suprachiasmatic Nucleus in Vitro
Endocrinology, November 1, 2002; 143(11): 4165 - 4171.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. P. Michael and C. R. McClung
Phase-Specific Circadian Clock Regulatory Elements in Arabidopsis
Plant Physiology, October 1, 2002; 130(2): 627 - 638.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
N. Mutsuga, Y. Iwasaki, M. Morishita, A. Nomura, E. Yamamori, M. Yoshida, M. Asai, N. Ozaki, F. Kambe, H. Seo, et al.
Homeobox Protein Gsh-1-Dependent Regulation of the Rat GHRH Gene Promoter
Mol. Endocrinol., December 1, 2001; 15(12): 2149 - 2156.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. P. H. Burbach, S. M. Luckman, D. Murphy, and H. Gainer
Gene Regulation in the Magnocellular Hypothalamo-Neurohypophysial System
Physiol Rev, July 1, 2001; 81(3): 1197 - 1267.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
A. Lacroix, N. N'Diaye, J. Tremblay, and P. Hamet
Ectopic and Abnormal Hormone Receptors in Adrenal Cushing's Syndrome
Endocr. Rev., February 1, 2001; 22(1): 75 - 110.
[Abstract] [Full Text]

Home page
 Mol. Pharmacol.Home page
X. Xin, N. Yang, and J. E. Faber
Platelet-Derived Growth Factor-BB Inhibits Rat alpha 1D-Adrenergic Receptor Gene Expression in Vascular Smooth Muscle Cells by Inducing AP-2-Like Protein Binding to alpha 1D Proximal Promoter Region
Mol. Pharmacol., December 1, 1999; 56(6): 1152 - 1161.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
K. Itoi, D. L. Helmreich, M. O. Lopez-Figueroa, and S. J. Watson
Differential Regulation of Corticotropin-Releasing Hormone and Vasopressin Gene Transcription in the Hypothalamus by Norepinephrine
J. Neurosci., July 1, 1999; 19(13): 5464 - 5472.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Obrietan, S. Impey, D. Smith, J. Athos, and D. R. Storm
Circadian Regulation of cAMP Response Element-mediated Gene Expression in the Suprachiasmatic Nuclei
J. Biol. Chem., June 18, 1999; 274(25): 17748 - 17756.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Wölfl, C. Martinez, and J. A. Majzoub
Inducible Binding of Cyclic Adenosine 3',5'-Monophosphate (cAMP)-Responsive Element Binding Protein (CREB) to a cAMP-Responsive Promoter in Vivo
Mol. Endocrinol., May 1, 1999; 13(5): 659 - 669.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
R. L. Emanuel, Y. Iwasaki, Z. K. Arbiser, E. M. Velez, C. H. Emerson, and J. A. Majzoub
Vasopressin Messenger Ribonucleic Acid Regulation via the Protein Kinase A Pathway
Endocrinology, June 1, 1998; 139(6): 2831 - 2837.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society